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Found 1 Document (1.393 seconds)

BMRB: 6133

1SR2

1H, 13C, and 15N Chemical Shift Assignments for YojN-HPt

Authors

Rogov, V.V., Loehr, F., Bernhard, F., Doetsch, V.

Assembly

YojN-HPt

Entity

1. YojN-HPt (polymer, Thiol state: all free), 116 monomers, 12836.41 Da Detail

MQEAVLQLIE VQLAQEEVTE SPLGGDENAQ LHASGYYALF VDTVPDDVKR LYTEAATSDF AALAQTAHRL KGVFAMLNLV PGKQLCETLE HLIREKDVPG IEKYISDIDS YVKSLL

Formula weight

12836.41 Da

Source organism

Escherichia coli

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 94.2 %, Completeness (bb): 97.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	94.2 % (1253 of 1330)	91.2 % (623 of 683)	97.3 % (512 of 526)	97.5 % (118 of 121)
Backbone	97.1 % (668 of 688)	96.6 % (226 of 234)	97.4 % (333 of 342)	97.3 % (109 of 112)
Sidechain	92.0 % (692 of 752)	88.4 % (397 of 449)	97.3 % (286 of 294)	100.0 % (9 of 9)
Aromatic	82.9 % (68 of 82)	65.9 % (27 of 41)	100.0 % (41 of 41)
Methyl	98.1 % (157 of 160)	98.8 % (79 of 80)	97.5 % (78 of 80)

1. YojN-HPt

MQEAVLQLIE VQLAQEEVTE SPLGGDENAQ LHASGYYALF VDTVPDDVKR LYTEAATSDF AALAQTAHRL KGVFAMLNLV PGKQLCETLE HLIREKDVPG IEKYISDIDS YVKSLL

Show sample condition

Sample #1

Temperature 293 (±0.5) K, pH 7.0 (±0.05)

#	Name	Concentration
1	YojN-HPt	1 mM
2	Bis-Tris	15 mM
3	NaCl	15 mM
4	H2O	99.99 %

Sample #2

Temperature 293 (±0.5) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Concentration
5	YojN-HPt	[U-95% 13C; U-95% 15N]	1 mM
6	Bis-Tris		15 mM
7	NaCl		15 mM
8	H2O		5 %
9	D2O		95 %

Release date

2004-09-29

Citation

Assignment of (1)H, (13)C and (15)N resonances of the Escherichia coli YojN histidine-phosphotransferase (HPt) domain
Rogov, V.V., Loehr, F., Bernhard, F., Doetsch, V.
J. Biomol. NMR (2004), 30, 103-104, PubMed 15452440 , DOI 10.1023/B:JNMR.0000042964.09428.4e , Abstract

Related entities 1. YojN-HPt, : 1 : 2 entities Detail

Interaction partners 1. YojN-HPt, : 5 interactors Detail

More details for 5 interactors identified by 2 citations

Experiments performed 8 experiments Detail

1 3D [15N, 1H]-TROSY-HNCO

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker Avance (700 MHz)

Sample #1

Temperature 293 (±0.5) K, pH 7.0 (±0.05)

#	Name	Concentration
1	YojN-HPt	1 mM
2	Bis-Tris	15 mM
3	NaCl	15 mM
4	H2O	99.99 %

Sample #2

Temperature 293 (±0.5) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Concentration
5	YojN-HPt	[U-95% 13C; U-95% 15N]	1 mM
6	Bis-Tris		15 mM
7	NaCl		15 mM
8	H2O		5 %
9	D2O		95 %

2 3D (HCA)CO(CA)NH

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker Avance (700 MHz)

Sample #1

Temperature 293 (±0.5) K, pH 7.0 (±0.05)

#	Name	Concentration
1	YojN-HPt	1 mM
2	Bis-Tris	15 mM
3	NaCl	15 mM
4	H2O	99.99 %

Sample #2

Temperature 293 (±0.5) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Concentration
5	YojN-HPt	[U-95% 13C; U-95% 15N]	1 mM
6	Bis-Tris		15 mM
7	NaCl		15 mM
8	H2O		5 %
9	D2O		95 %

3 3D [15N, 1H]-TROSY-HNCACB

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker Avance (700 MHz)

Sample #1

Temperature 293 (±0.5) K, pH 7.0 (±0.05)

#	Name	Concentration
1	YojN-HPt	1 mM
2	Bis-Tris	15 mM
3	NaCl	15 mM
4	H2O	99.99 %

Sample #2

Temperature 293 (±0.5) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Concentration
5	YojN-HPt	[U-95% 13C; U-95% 15N]	1 mM
6	Bis-Tris		15 mM
7	NaCl		15 mM
8	H2O		5 %
9	D2O		95 %

4 3D HBHA(CBCA)(CO)NH

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker Avance (700 MHz)

Sample #1

Temperature 293 (±0.5) K, pH 7.0 (±0.05)

#	Name	Concentration
1	YojN-HPt	1 mM
2	Bis-Tris	15 mM
3	NaCl	15 mM
4	H2O	99.99 %

Sample #2

Temperature 293 (±0.5) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Concentration
5	YojN-HPt	[U-95% 13C; U-95% 15N]	1 mM
6	Bis-Tris		15 mM
7	NaCl		15 mM
8	H2O		5 %
9	D2O		95 %

5 3D H(CC)(CO)NH-TOCSY

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker Avance (700 MHz)

Sample #1

Temperature 293 (±0.5) K, pH 7.0 (±0.05)

#	Name	Concentration
1	YojN-HPt	1 mM
2	Bis-Tris	15 mM
3	NaCl	15 mM
4	H2O	99.99 %

Sample #2

Temperature 293 (±0.5) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Concentration
5	YojN-HPt	[U-95% 13C; U-95% 15N]	1 mM
6	Bis-Tris		15 mM
7	NaCl		15 mM
8	H2O		5 %
9	D2O		95 %

6 3D (H)C(C)(CO)NH-TOCSY

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker Avance (700 MHz)

Sample #1

Temperature 293 (±0.5) K, pH 7.0 (±0.05)

#	Name	Concentration
1	YojN-HPt	1 mM
2	Bis-Tris	15 mM
3	NaCl	15 mM
4	H2O	99.99 %

Sample #2

Temperature 293 (±0.5) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Concentration
5	YojN-HPt	[U-95% 13C; U-95% 15N]	1 mM
6	Bis-Tris		15 mM
7	NaCl		15 mM
8	H2O		5 %
9	D2O		95 %

7 3D 15N-edited TOCSY-HSQC

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker Avance (700 MHz)

Sample #1

Temperature 293 (±0.5) K, pH 7.0 (±0.05)

#	Name	Concentration
1	YojN-HPt	1 mM
2	Bis-Tris	15 mM
3	NaCl	15 mM
4	H2O	99.99 %

Sample #2

Temperature 293 (±0.5) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Concentration
5	YojN-HPt	[U-95% 13C; U-95% 15N]	1 mM
6	Bis-Tris		15 mM
7	NaCl		15 mM
8	H2O		5 %
9	D2O		95 %

8 2D TOCSY in 2H2O

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker Avance (700 MHz)

Sample #1

Temperature 293 (±0.5) K, pH 7.0 (±0.05)

#	Name	Concentration
1	YojN-HPt	1 mM
2	Bis-Tris	15 mM
3	NaCl	15 mM
4	H2O	99.99 %

Sample #2

Temperature 293 (±0.5) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Concentration
5	YojN-HPt	[U-95% 13C; U-95% 15N]	1 mM
6	Bis-Tris		15 mM
7	NaCl		15 mM
8	H2O		5 %
9	D2O		95 %

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr6133_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr6133_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	shift_1	Warning	1429		100.0 (chain: 1, length: 116)
1	Chemical shift references	chemical_shift_reference	OK	3		No information

Assigned chemical shifts

Saveframe: shift_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 116, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 784
  - ¹⁵N chemical shifts: 121
  - ¹³C chemical shifts: 524
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	683	624	91.4
¹⁵N chemical shifts	124	121	97.6
¹³C chemical shifts	526	524	99.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	234	232	99.1
¹⁵N chemical shifts	112	112	100.0
¹³C chemical shifts	232	232	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	449	392	87.3
¹⁵N chemical shifts	12	9	75.0
¹³C chemical shifts	294	292	99.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	82	80	97.6
¹³C chemical shifts	82	80	97.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	41	25	61.0
¹³C chemical shifts	41	41	100.0

Keywords sensor kinase, NMR

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Found 1 Document (1.393 seconds)

BMRB: 6133

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Related entities 1. YojN-HPt, : 1 : 2 entities Detail

Interaction partners 1. YojN-HPt, : 5 interactors Detail

Experiments performed 8 experiments Detail

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Chemical shift validation 3 contents Detail