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BMRB: 6158

1SN6

NMR solution structure of human Saposin C in the presence of detergent micelles

Authors

Hawkins, C.A., de Alba, E., Tjandra, N.

Assembly

saposin C

Entity

1. saposin C (polymer, Thiol state: all disulfide bound), 84 monomers, 9415.822 Da Detail

SDVYCEVCEF LVKEVTKLID NNKTEKEILD AFDKMCSKLP KSLSEECQEV VDTYGSSILS ILLEEVSPEL VCSMLHLCSG LVPR

Formula weight

9415.822 Da

Entity Connection

disulfide 3 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS5:SG	1:CYS78:SG
2	disulfide	sing	1:CYS8:SG	1:CYS72:SG
3	disulfide	sing	1:CYS36:SG	1:CYS47:SG

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

distance geometry

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 92.8 %, Completeness (bb): 96.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	92.8 % (900 of 970)	88.4 % (444 of 502)	98.4 % (378 of 384)	92.9 % (78 of 84)
Backbone	96.6 % (481 of 498)	96.4 % (161 of 167)	98.0 % (245 of 250)	92.6 % (75 of 81)
Sidechain	90.4 % (501 of 554)	84.5 % (283 of 335)	99.5 % (215 of 216)	100.0 % (3 of 3)
Aromatic	100.0 % (40 of 40)	100.0 % (20 of 20)	100.0 % (20 of 20)
Methyl	100.0 % (108 of 108)	100.0 % (54 of 54)	100.0 % (54 of 54)

1. saposin C

SDVYCEVCEF LVKEVTKLID NNKTEKEILD AFDKMCSKLP KSLSEECQEV VDTYGSSILS ILLEEVSPEL VCSMLHLCSG LVPR

Show sample condition

Sample

Temperature 298 (±1) K, pH 6.8 (±0.1), Details 25 mM sodium dodecyl sulfate was the micelle material

#	Name	Isotope labeling	Type	Concentration
1	saposin C	[U-100% 13C; U-100% 15N]		1.0 mM
2	sodium dodecyl sulfate			25 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.09 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.09 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.16 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1SN6, Strand ID: A Detail

Release date

2004-03-21

Citation 1

Solution structure of human saposin C in a detergent environment
Hawkins, C.A., de Alba, E., Tjandra, N.
J. Mol. Biol. (2005), 346, 1381-1392, PubMed 15713488 , DOI 10.1016/j.jmb.2004.12.045 , Abstract

Show more 1 citaion

Citation 2

Solution structure of human saposin C: pH-dependent interaction with phospholipid vesicles
de Alba, E., Weiler, S., Tjandra, N.
Biochemistry (2003), 42, 14729-14740, PubMed 14674747 , DOI 10.1021/bi0301338 , Abstract

Hide non-primary 1 citaion

Related entities 1. saposin C, : 1 : 1 : 5 : 184 entities Detail

Interaction partners 1. saposin C, : 19 interactors Detail

More details for 19 interactors identified by 12 citations

Experiments performed 9 experiments Detail

1 CBCACONH