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BMRB: 6173

2LP6

PfR48 final project

Authors

Assembly

PfR48

Entity

1. PfR48 (polymer, Thiol state: not present), 95 monomers, 10800.50 Da Detail

MRIKGVVLSY RRSKENQHNN VMIIKPLDVN SREEASKLIG RLVLWKSPSG KILKGKIVRV HGTKGAVRAR FEKGLPGQAL GDYVEIVLEH HHHHH

Formula weight

10800.5 Da

Source organism

Pyrococcus furiosus

Exptl. method

Refine. method

simulated annealing

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete1

Sequence coverage: 94.7 %, Completeness: 86.6 %, Completeness (bb): 92.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	86.6 % (992 of 1145)	86.4 % (521 of 603)	85.6 % (379 of 443)	92.9 % (92 of 99)
Backbone	92.4 % (521 of 564)	92.3 % (181 of 196)	92.0 % (254 of 276)	93.5 % (86 of 92)
Sidechain	82.5 % (550 of 667)	83.5 % (340 of 407)	80.6 % (204 of 253)	85.7 % (6 of 7)
Aromatic	0.0 % (0 of 70)	0.0 % (0 of 35)	0.0 % (0 of 34)	0.0 % (0 of 1)
Methyl	100.0 % (114 of 114)	100.0 % (57 of 57)	100.0 % (57 of 57)

1. PfR48

MRIKGVVLSY RRSKENQHNN VMIIKPLDVN SREEASKLIG RLVLWKSPSG KILKGKIVRV HGTKGAVRAR FEKGLPGQAL GDYVEIVLEH HHHHH

Show sample condition

Sample

Temperature 293 (±0.1) K, pH 6.5 (±0.5)

#	Name	Isotope labeling	Type	Concentration
1	PfR48	[U-15N]		1.07 mM
2	NaCl			100 mM
3	CaCl2			5 mM

Chem. Shift Complete2

Sequence coverage: 12.6 %, Completeness: 48.4 %, Completeness (bb): 50.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	48.4 % (1109 of 2290)	48.3 % (582 of 1206)	48.1 % (426 of 886)	51.0 % (101 of 198)
Backbone	50.3 % (567 of 1128)	50.8 % (199 of 392)	49.6 % (274 of 552)	51.1 % (94 of 184)
Sidechain	47.0 % (627 of 1334)	47.1 % (383 of 814)	46.8 % (237 of 506)	50.0 % (7 of 14)
Aromatic	27.1 % (38 of 140)	27.1 % (19 of 70)	26.5 % (18 of 68)	50.0 % (1 of 2)
Methyl	53.1 % (121 of 228)	53.5 % (61 of 114)	52.6 % (60 of 114)

1. PfR48

MRIKGVVLSY RRSKENQHNN VMIIKPLDVN SREEASKLIG RLVLWKSPSG KILKGKIVRV HGTKGAVRAR FEKGLPGQAL GDYVEIVLEH HHHHH

Show sample condition

Sample

Temperature 293 (±0.1) K, pH 6.5 (±0.5)

#	Name	Isotope labeling	Type	Concentration
1	PfR48	[U-15N]		1.07 mM
2	NaCl			100 mM
3	CaCl2			5 mM

Protein Blocks Logo

Calculated from 20 models in PDB: 2LP6, Strand ID: A Detail

Coupling constant

42 J values in 1 lists
Field strength (¹H) 750 MHz, Temperature 293 (±0.1) K, pH 6.5 (±0.5) Detail

Release date

2006-02-25

Citation

Solution Structure of the 50S Ribosomal Protein L35Ae from Pyrococcus furiosus: Northeast Strucutral Genomics Consortium target: Pfr48
Snyder, D., Aramini, J.M., Huang, Y.J., Xiao, R., Cort, J.R., Shastry, R., Ma, L.C., Liu, J., Rost, B., Acton, T.B., Kennedy, M.A., Montelione, G.T.

Related entities 1. PfR48, : 1 : 1 : 3 : 204 entities Detail

Experiments performed 32 experiments Detail

1 HNCA

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Temperature 293 (±0.1) K, pH 6.5 (±0.5)

#	Name	Isotope labeling	Type	Concentration
1	PfR48	[U-15N]		1.07 mM
2	NaCl			100 mM
3	CaCl2			5 mM

2 HNCACB

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Temperature 293 (±0.1) K, pH 6.5 (±0.5)

#	Name	Isotope labeling	Type	Concentration
1	PfR48	[U-15N]		1.07 mM
2	NaCl			100 mM
3	CaCl2			5 mM

3 HNCO

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Temperature 293 (±0.1) K, pH 6.5 (±0.5)

#	Name	Isotope labeling	Type	Concentration
1	PfR48	[U-15N]		1.07 mM
2	NaCl			100 mM
3	CaCl2			5 mM

4 NH_HSQC

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Temperature 293 (±0.1) K, pH 6.5 (±0.5)

#	Name	Isotope labeling	Type	Concentration
1	PfR48	[U-15N]		1.07 mM
2	NaCl			100 mM
3	CaCl2			5 mM

5 NH_HSQC

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Temperature 293 (±0.1) K, pH 6.5 (±0.5)

#	Name	Isotope labeling	Type	Concentration
1	PfR48	[U-15N]		1.07 mM
2	NaCl			100 mM
3	CaCl2			5 mM

6 HNcoCA

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Temperature 293 (±0.1) K, pH 6.5 (±0.5)

#	Name	Isotope labeling	Type	Concentration
1	PfR48	[U-15N]		1.07 mM
2	NaCl			100 mM
3	CaCl2			5 mM

7 HNcoCACB

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Temperature 293 (±0.1) K, pH 6.5 (±0.5)

#	Name	Isotope labeling	Type	Concentration
1	PfR48	[U-15N]		1.07 mM
2	NaCl			100 mM
3	CaCl2			5 mM

8 HAcacoNH

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Temperature 293 (±0.1) K, pH 6.5 (±0.5)

#	Name	Isotope labeling	Type	Concentration
1	PfR48	[U-15N]		1.07 mM
2	NaCl			100 mM
3	CaCl2			5 mM

9 HAcaNH

Instrument

Varian Baker - 600 MHz

Sample

State isotropic, Temperature 293 (±0.1) K, pH 6.5 (±0.5)

#	Name	Isotope labeling	Type	Concentration
1	PfR48	[U-15N]		1.07 mM
2	NaCl			100 mM
3	CaCl2			5 mM

10 HNCACB (tau c)

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Temperature 293 (±0.1) K, pH 6.5 (±0.5)

#	Name	Isotope labeling	Type	Concentration
1	PfR48	[U-15N]		1.07 mM
2	NaCl			100 mM
3	CaCl2			5 mM

11 HNHA

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Temperature 293 (±0.1) K, pH 6.5 (±0.5)

#	Name	Isotope labeling	Type	Concentration
1	PfR48	[U-15N]		1.07 mM
2	NaCl			100 mM
3	CaCl2			5 mM

12 CCONH

Instrument

Varian Baker - 600 MHz

Sample

State isotropic, Temperature 293 (±0.1) K, pH 6.5 (±0.5)

#	Name	Isotope labeling	Type	Concentration
1	PfR48	[U-15N]		1.07 mM
2	NaCl			100 mM
3	CaCl2			5 mM

13 HCCH-COSY

Instrument

Varian Baker - 600 MHz

Sample

State isotropic, Temperature 293 (±0.1) K, pH 6.5 (±0.5)

#	Name	Isotope labeling	Type	Concentration
1	PfR48	[U-15N]		1.07 mM
2	NaCl			100 mM
3	CaCl2			5 mM

14 HCCH-TOCSY

Instrument

Varian Baker - 600 MHz

Sample

State isotropic, Temperature 293 (±0.1) K, pH 6.5 (±0.5)

#	Name	Isotope labeling	Type	Concentration
1	PfR48	[U-15N]		1.07 mM
2	NaCl			100 mM
3	CaCl2			5 mM

15 hetNOE (t0)

Instrument

Varian Baker - 600 MHz

Sample

State isotropic, Temperature 293 (±0.1) K, pH 6.5 (±0.5)

#	Name	Isotope labeling	Type	Concentration
1	PfR48	[U-15N]		1.07 mM
2	NaCl			100 mM
3	CaCl2			5 mM

16 hetNOE (t3)

Instrument

Varian Baker - 600 MHz

Sample

State isotropic, Temperature 293 (±0.1) K, pH 6.5 (±0.5)

#	Name	Isotope labeling	Type	Concentration
1	PfR48	[U-15N]		1.07 mM
2	NaCl			100 mM
3	CaCl2			5 mM

17 CH_HSQC (aliph)

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Temperature 293 (±0.1) K, pH 6.5 (±0.5)

#	Name	Isotope labeling	Type	Concentration
1	PfR48	[U-15N]		1.07 mM
2	NaCl			100 mM
3	CaCl2			5 mM

18 CH_HSQC (aliph)

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Temperature 293 (±0.1) K, pH 6.5 (±0.5)

#	Name	Isotope labeling	Type	Concentration
1	PfR48	[U-15N]		1.07 mM
2	NaCl			100 mM
3	CaCl2			5 mM

19 hbCBcgcdceHE

Instrument

Varian Baker - 600 MHz

Sample

State isotropic, Temperature 293 (±0.1) K, pH 6.5 (±0.5)

#	Name	Isotope labeling	Type	Concentration
1	PfR48	[U-15N]		1.07 mM
2	NaCl			100 mM
3	CaCl2			5 mM

20 HBCBcgcdHD

Instrument

Varian Baker - 600 MHz

Sample

State isotropic, Temperature 293 (±0.1) K, pH 6.5 (±0.5)

#	Name	Isotope labeling	Type	Concentration
1	PfR48	[U-15N]		1.07 mM
2	NaCl			100 mM
3	CaCl2			5 mM

21 MUSIC_gly_CA

Instrument

Varian Baker - 600 MHz

Sample

State isotropic, Temperature 293 (±0.1) K, pH 6.5 (±0.5)

#	Name	Isotope labeling	Type	Concentration
1	PfR48	[U-15N]		1.07 mM
2	NaCl			100 mM
3	CaCl2			5 mM

22 MUSIC_gly_CO

Instrument

Varian Baker - 600 MHz

Sample

State isotropic, Temperature 293 (±0.1) K, pH 6.5 (±0.5)

#	Name	Isotope labeling	Type	Concentration
1	PfR48	[U-15N]		1.07 mM
2	NaCl			100 mM
3	CaCl2			5 mM

23 CH_HSQC (arom)

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Temperature 293 (±0.1) K, pH 6.5 (±0.5)

#	Name	Isotope labeling	Type	Concentration
1	PfR48	[U-15N]		1.07 mM
2	NaCl			100 mM
3	CaCl2			5 mM

24 15N-edited_NOESY

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Temperature 293 (±0.1) K, pH 6.5 (±0.5)

#	Name	Isotope labeling	Type	Concentration
1	PfR48	[U-15N]		1.07 mM
2	NaCl			100 mM
3	CaCl2			5 mM

25 13C_edited_NOESY (aliph)

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Temperature 293 (±0.1) K, pH 6.5 (±0.5)

#	Name	Isotope labeling	Type	Concentration
1	PfR48	[U-15N]		1.07 mM
2	NaCl			100 mM
3	CaCl2			5 mM

26 13C_edited_NOESY (D2O aliph)

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Temperature 293 (±0.1) K, pH 6.5 (±0.5)

#	Name	Isotope labeling	Type	Concentration
1	PfR48	[U-15N]		1.07 mM
2	NaCl			100 mM
3	CaCl2			5 mM

27 13C_edited_NOESY (arom)

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Temperature 293 (±0.1) K, pH 6.5 (±0.5)

#	Name	Isotope labeling	Type	Concentration
1	PfR48	[U-15N]		1.07 mM
2	NaCl			100 mM
3	CaCl2			5 mM

28 HCCcoNH-TOCSY

Instrument

Varian Baker - 600 MHz

Sample

State isotropic, Temperature 293 (±0.1) K, pH 6.5 (±0.5)

#	Name	Isotope labeling	Type	Concentration
1	PfR48	[U-15N]		1.07 mM
2	NaCl			100 mM
3	CaCl2			5 mM

29 4D-C13-NOESY

Instrument

Varian Baker - 600 MHz

Sample

State isotropic, Temperature 293 (±0.1) K, pH 6.5 (±0.5)

#	Name	Isotope labeling	Type	Concentration
1	PfR48	[U-15N]		1.07 mM
2	NaCl			100 mM
3	CaCl2			5 mM

30 NH_HSQC (larger SW1)

Instrument

Varian UNITY Inova - 500 MHz

Sample

State isotropic, Temperature 293 (±0.1) K, pH 6.5 (±0.5)

#	Name	Isotope labeling	Type	Concentration
4	PfR48	[U-15N]		0.29 mM
5	NaCl			100 mM
6	CaCl2			5 mM

31 CH_HSQC

Instrument

Varian UNITY Inova - 600 MHz

Sample

State isotropic, Temperature 293 (±0.1) K, pH 6.5 (±0.5)

#	Name	Isotope labeling	Type	Concentration
4	PfR48	[U-15N]		0.29 mM
5	NaCl			100 mM
6	CaCl2			5 mM

32 NH_HSQC (15 min)

Instrument

Varian UNITY Inova - 600 MHz

Sample

State isotropic, Temperature 293 (±0.1) K, pH 6.5 (±0.5)

#	Name	Isotope labeling	Type	Concentration
7	PfR48	[U-15N]		0.5 mM
8	NaCl			100 mM
9	CaCl2			5 mM

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