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Solution structure of the 15th domain of LEKTI
Authors
Vitzithum, K., Roesch, P., Marx, U.C.
Assembly
LEKTI Domain 15
Entity
1. LEKTI Domain 15 (polymer, Thiol state: all disulfide bound), 78 monomers, 8575.550 Da Detail

GPDSEMCKDY RVLPRIGYLC PKDLKPVCGD DGQTYNNPCM LCHENLIRQT NTHIRSTGKC EESSTPGTTA ASMPPSDE


Formula weight
8575.55 Da
Entity Connection
disulfide 3 Detail

IDTypeValue orderAtom ID 1Atom ID 2
1disulfidesing1:CYS7:SG1:CYS42:SG
2disulfidesing1:CYS20:SG1:CYS39:SG
3disulfidesing1:CYS28:SG1:CYS60:SG

Source organism
Homo sapiens
Exptl. method
NMR
Refine. method
SIMULATED ANNEALING
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 97.4 %, Completeness: 85.0 %, Completeness (bb): 97.3 % Detail

Polymer type: polypeptide(L)

Total1H15N
All85.0 % (446 of 525)82.6 % (371 of 449)98.7 % (75 of 76)
Backbone97.3 % (218 of 224)96.8 % (149 of 154)98.6 % (69 of 70)
Sidechain75.7 % (228 of 301)75.3 % (222 of 295)100.0 % (6 of 6)
Aromatic75.0 % (12 of 16)75.0 % (12 of 16)
Methyl100.0 % (29 of 29)100.0 % (29 of 29)

1. LEKTI Domain 15

GPDSEMCKDY RVLPRIGYLC PKDLKPVCGD DGQTYNNPCM LCHENLIRQT NTHIRSTGKC EESSTPGTTA ASMPPSDE

Sample

Pressure 1 atm, Temperature 298 (±1) K, pH 6.4 (±0.1)


#NameIsotope labelingTypeConcentration
1LEKTI Domain 15[U-15N]1.6 mM

Protein Blocks Logo
Calculated from 31 models in PDB: 1UVG, Strand ID: A Detail


Release date
2005-05-16
Citation
The solution structure of a chimeric LEKTI domain reveals a chameleon sequence
Tidow, H., Lauber, T., Vitzithum, K., Sommerhoff, C.P., Roesch, P., Marx, U.C.
Biochemistry (2004), 43, 11238-11247, PubMed 15366933 , DOI 10.1021/bi0492399 ,
Related entities 1. LEKTI Domain 15, : 1 : 2 : 20 entities Detail
Experiments performed 8 experiments Detail
nullKeywords Serine Proteinase Inhibitor, Trypsin Inhibitor, Kazal, LEKTI