BMRBj - BMREntryMaster

Found 1 Document (2.047 seconds)

BMRB: 6225

2GS0

1H, 15N, and 13C Resonance Assignment of the Amino-terminal Domain of Tfb1 subunit of yeast TFIIH

Authors

Nguyen, B.D., Di Lello, P., Legault, P., Ominchinski, J.G.

Assembly

Tfb1

Entity

1. Tfb1 (polymer, Thiol state: not present), 119 monomers, 13228.85 Da Detail

PSHSGAAIFE KVSGIIAINE DVSPAELTWR STDGDKVHTV VLSTIDKLQA TPASSEKMML RLIGKVDESK KRKDNEGNEV VPKPQRHMFS FNNRTVMDNI KMTLQQIISR YKDADGNSS

Formula weight

13228.85 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

NMR

Refine. method

Simulated annealing, with a combination of torsion angle and Cartesian dynamics.

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.3 %, Completeness: 93.9 %, Completeness (bb): 95.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	93.9 % (1287 of 1371)	94.8 % (681 of 718)	92.0 % (485 of 527)	96.0 % (121 of 126)
Backbone	95.0 % (669 of 704)	95.8 % (229 of 239)	94.3 % (331 of 351)	95.6 % (109 of 114)
Sidechain	93.1 % (726 of 780)	94.4 % (452 of 479)	90.7 % (262 of 289)	100.0 % (12 of 12)
Aromatic	72.6 % (45 of 62)	87.1 % (27 of 31)	56.7 % (17 of 30)	100.0 % (1 of 1)
Methyl	93.5 % (116 of 124)	93.5 % (58 of 62)	93.5 % (58 of 62)

1. Yeast Tfb1

PSHSGAAIFE KVSGIIAINE DVSPAELTWR STDGDKVHTV VLSTIDKLQA TPASSEKMML RLIGKVDESK KRKDNEGNEV VPKPQRHMFS FNNRTVMDNI KMTLQQIISR YKDADGNSS

Show sample condition

Sample

Temperature 300 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	Yeast Tfb1			1.2 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.15 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.15 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.04 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.25 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2GS0, Strand ID: A Detail

Release date

2005-03-17

Citation

1H, 15N, and 13C resonance assignment of the amino-terminal domain of the Tfb1 subunit of yeast TFIIH
Nguyen, B.D., Di Lello, P., Legault, P., Ominchinski, J.G.
J. Biomol. NMR (2005), 31, 173-174, PubMed 15772758 , DOI 10.1007/s10858-004-7266-0 , Abstract

Related entities 1. Tfb1, : 1 : 2 : 32 : 5 entities Detail

Interaction partners 1. Tfb1, : 49 interactors Detail

More details for 49 interactors identified by 12 citations

Experiments performed 14 experiments Detail

1 1H-15N HSQC

Instrument

#1 Model Varian INOVA (800 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian INOVA (500 MHz)

Sample #1

Temperature 300 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	Yeast Tfb1			1.2 mM

Sample #2

Temperature 300 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
2	Yeast Tfb1	[U-98% 15N]		1.2 mM

Sample #3

Temperature 300 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
3	Yeast Tfb1	[U-98% 13C; U-98% 15N]		1.2 mM

2 1H-13C CT-HSQC

Instrument

#1 Model Varian INOVA (800 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian INOVA (500 MHz)

Sample #1

Temperature 300 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	Yeast Tfb1			1.2 mM

Sample #2

Temperature 300 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
2	Yeast Tfb1	[U-98% 15N]		1.2 mM

Sample #3

Temperature 300 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
3	Yeast Tfb1	[U-98% 13C; U-98% 15N]		1.2 mM

3 1H-15N NOESY-HSQC

Instrument

#1 Model Varian INOVA (800 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian INOVA (500 MHz)

Sample #1

Temperature 300 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	Yeast Tfb1			1.2 mM

Sample #2

Temperature 300 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
2	Yeast Tfb1	[U-98% 15N]		1.2 mM

Sample #3

Temperature 300 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
3	Yeast Tfb1	[U-98% 13C; U-98% 15N]		1.2 mM

4 HNCACB

Instrument

#1 Model Varian INOVA (800 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian INOVA (500 MHz)

Sample #1

Temperature 300 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	Yeast Tfb1			1.2 mM

Sample #2

Temperature 300 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
2	Yeast Tfb1	[U-98% 15N]		1.2 mM

Sample #3

Temperature 300 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
3	Yeast Tfb1	[U-98% 13C; U-98% 15N]		1.2 mM

5 (HB)CBCA(CO)NNH

Instrument

#1 Model Varian INOVA (800 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian INOVA (500 MHz)

Sample #1

Temperature 300 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	Yeast Tfb1			1.2 mM

Sample #2

Temperature 300 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
2	Yeast Tfb1	[U-98% 15N]		1.2 mM

Sample #3

Temperature 300 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
3	Yeast Tfb1	[U-98% 13C; U-98% 15N]		1.2 mM

6 HNCO

Instrument

#1 Model Varian INOVA (800 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian INOVA (500 MHz)

Sample #1

Temperature 300 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	Yeast Tfb1			1.2 mM

Sample #2

Temperature 300 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
2	Yeast Tfb1	[U-98% 15N]		1.2 mM

Sample #3

Temperature 300 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
3	Yeast Tfb1	[U-98% 13C; U-98% 15N]		1.2 mM

7 H(CCO)NNH-TOCSY

Instrument

#1 Model Varian INOVA (800 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian INOVA (500 MHz)

Sample #1

Temperature 300 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	Yeast Tfb1			1.2 mM

Sample #2

Temperature 300 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
2	Yeast Tfb1	[U-98% 15N]		1.2 mM

Sample #3

Temperature 300 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
3	Yeast Tfb1	[U-98% 13C; U-98% 15N]		1.2 mM

8 C(CO)NNH-TOCSY

Instrument

#1 Model Varian INOVA (800 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian INOVA (500 MHz)

Sample #1

Temperature 300 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	Yeast Tfb1			1.2 mM

Sample #2

Temperature 300 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
2	Yeast Tfb1	[U-98% 15N]		1.2 mM

Sample #3

Temperature 300 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
3	Yeast Tfb1	[U-98% 13C; U-98% 15N]		1.2 mM

9 1H-13C HMQC-NOESY

Instrument

#1 Model Varian INOVA (800 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian INOVA (500 MHz)

Sample #1

Temperature 300 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	Yeast Tfb1			1.2 mM

Sample #2

Temperature 300 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
2	Yeast Tfb1	[U-98% 15N]		1.2 mM

Sample #3

Temperature 300 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
3	Yeast Tfb1	[U-98% 13C; U-98% 15N]		1.2 mM

10 1H-15N NOESY_HSQC

Instrument

#1 Model Varian INOVA (800 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian INOVA (500 MHz)

Sample #1

Temperature 300 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	Yeast Tfb1			1.2 mM

Sample #2

Temperature 300 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
2	Yeast Tfb1	[U-98% 15N]		1.2 mM

Sample #3

Temperature 300 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
3	Yeast Tfb1	[U-98% 13C; U-98% 15N]		1.2 mM

11 NOESY

Instrument

#1 Model Varian INOVA (800 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian INOVA (500 MHz)

Sample #1

Temperature 300 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	Yeast Tfb1			1.2 mM

Sample #2

Temperature 300 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
2	Yeast Tfb1	[U-98% 15N]		1.2 mM

Sample #3

Temperature 300 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
3	Yeast Tfb1	[U-98% 13C; U-98% 15N]		1.2 mM

12 DQF-COSY

Instrument

#1 Model Varian INOVA (800 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian INOVA (500 MHz)

Sample #1

Temperature 300 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	Yeast Tfb1			1.2 mM

Sample #2

Temperature 300 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
2	Yeast Tfb1	[U-98% 15N]		1.2 mM

Sample #3

Temperature 300 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
3	Yeast Tfb1	[U-98% 13C; U-98% 15N]		1.2 mM

13 (HB)CB(CGCD)HD

Instrument

#1 Model Varian INOVA (800 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian INOVA (500 MHz)

Sample #1

Temperature 300 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	Yeast Tfb1			1.2 mM

Sample #2

Temperature 300 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
2	Yeast Tfb1	[U-98% 15N]		1.2 mM

Sample #3

Temperature 300 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
3	Yeast Tfb1	[U-98% 13C; U-98% 15N]		1.2 mM

14 (HB)CB(CGCDCE)HE

Instrument

#1 Model Varian INOVA (800 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian INOVA (500 MHz)

Sample #1

Temperature 300 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	Yeast Tfb1			1.2 mM

Sample #2

Temperature 300 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
2	Yeast Tfb1	[U-98% 15N]		1.2 mM

Sample #3

Temperature 300 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
3	Yeast Tfb1	[U-98% 13C; U-98% 15N]		1.2 mM

nullKeywords TFIIH, Tfb1, p62, NMR assignment

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (2.047 seconds)

BMRB: 6225

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Tfb1, : 1 : 2 : 32 : 5 entities Detail

Interaction partners 1. Tfb1, : 49 interactors Detail

Experiments performed 14 experiments Detail

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2