BMRB: 6251

Backbone Chemical Shift Assignments for V66W121 fragment of Staphylococcal Nuclease

Authors

Feng, Y., Liu, D., Wang, J.

Assembly

V66W121

Entity

1. V66W121 (polymer, Thiol state: not present), 121 monomers, 13675.70 Da Detail

ATSTKKLHKE PATLIKAIDG DTVKLMYKGQ PMTFRLLLVD TPETKHPKKG VEKYGPEASA FTKKMWENAK KIEVEFDKGQ RTDKYGRGLA YIYADGKMVN EALVRQGLAK VAYVYKPNNT H

Formula weight

13675.7 Da

Source organism

Staphylococcus aureus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 67.8 %, Completeness: 29.8 %, Completeness (bb): 52.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	29.8 % (435 of 1458)	23.9 % (183 of 766)	29.9 % (170 of 569)	66.7 % (82 of 123)
Backbone	52.5 % (375 of 714)	55.5 % (136 of 245)	44.6 % (158 of 354)	70.4 % (81 of 115)
Sidechain	15.5 % (133 of 856)	9.2 % (48 of 521)	25.7 % (84 of 327)	12.5 % (1 of 8)
Aromatic	0.0 % (0 of 110)	0.0 % (0 of 55)	0.0 % (0 of 54)	0.0 % (0 of 1)
Methyl	17.5 % (22 of 126)	14.3 % (9 of 63)	20.6 % (13 of 63)

1. V66W121

ATSTKKLHKE PATLIKAIDG DTVKLMYKGQ PMTFRLLLVD TPETKHPKKG VEKYGPEASA FTKKMWENAK KIEVEFDKGQ RTDKYGRGLA YIYADGKMVN EALVRQGLAK VAYVYKPNNT H

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Sample

Temperature 298 (±0.2) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	V66W121	[U-95% 13C; U-95% 15N]		1 mM
2	deuterated acetate	[U-99% 2H]		50 mM
3	DSS			50 uM
4	NaN3			0.02 %

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Chem. Shift Complete2

Sequence coverage: 32.2 %, Completeness: 18.6 %, Completeness (bb): 33.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	18.6 % (542 of 2916)	15.3 % (234 of 1532)	16.7 % (190 of 1138)	48.0 % (118 of 246)
Backbone	33.1 % (472 of 1428)	36.3 % (178 of 490)	25.1 % (178 of 708)	50.4 % (116 of 230)
Sidechain	8.7 % (149 of 1712)	5.5 % (57 of 1042)	13.8 % (90 of 654)	12.5 % (2 of 16)
Aromatic	0.0 % (0 of 220)	0.0 % (0 of 110)	0.0 % (0 of 108)	0.0 % (0 of 2)
Methyl	8.7 % (22 of 252)	7.1 % (9 of 126)	10.3 % (13 of 126)

1. V66W121

ATSTKKLHKE PATLIKAIDG DTVKLMYKGQ PMTFRLLLVD TPETKHPKKG VEKYGPEASA FTKKMWENAK KIEVEFDKGQ RTDKYGRGLA YIYADGKMVN EALVRQGLAK VAYVYKPNNT H

Show sample condition

Sample

Temperature 298 (±0.2) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	V66W121	[U-95% 13C; U-95% 15N]		1 mM
2	deuterated acetate	[U-99% 2H]		50 mM
3	DSS			50 uM
4	NaN3			0.02 %

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Release date

2004-08-02

Citation

Native-like partially folded conformations and folding process revealed in the N-terminal large fragments of staphylococcal nuclease: a study by NMR spectroscopy
Feng, Y., Liu, D., Wang, J.
J. Mol. Biol. (2003), 330, 821-837, PubMed 12850150 , DOI: , Abstract

Related entities 1. V66W121, : 1 : 66 : 52 entities Detail

Experiments performed 6 experiments Detail

Chemical shift validation 4 contents Detail