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Found 1 Document (1.788 seconds)

BMRB: 6254

1TXE

1H, 13C, and 15N Chemical Shift Assignments for I14A mutant of histidine-containing phosphocarrier protein from Staphylococcus carnosus

Authors

Moglich, A., Koch, B., Hengstenberg, W., Brunner, E., Kalbitzer, H.

Assembly

HPr monomer

Entity

1. HPr monomer (polymer, Thiol state: not present), 88 monomers, 9468.503 Da Detail

MEQQSYTIID ETGAHARPAT MLVQTASKFD SDIQLEYNGK KVNLKSIMGV MSLGVGKDAE ITIYADGSDE ADAIQAITDV LSKEGLTE

Formula weight

9468.503 Da

Source organism

Staphylococcus carnosus

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 88.3 %, Completeness (bb): 98.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	88.3 % (854 of 967)	96.4 % (478 of 496)	75.1 % (283 of 377)	98.9 % (93 of 94)
Backbone	98.5 % (518 of 526)	98.4 % (179 of 182)	98.4 % (253 of 257)	98.9 % (86 of 87)
Sidechain	79.5 % (415 of 522)	95.2 % (299 of 314)	54.2 % (109 of 201)	100.0 % (7 of 7)
Aromatic	47.4 % (18 of 38)	94.7 % (18 of 19)	0.0 % (0 of 19)
Methyl	74.1 % (80 of 108)	100.0 % (54 of 54)	48.1 % (26 of 54)

1. HPrI14A from S. carnosus

MEQQSYTIID ETGAHARPAT MLVQTASKFD SDIQLEYNGK KVNLKSIMGV MSLGVGKDAE ITIYADGSDE ADAIQAITDV LSKEGLTE

Show sample condition

Sample

Temperature 298 K, pH 7.14

#	Name	Isotope labeling	Concentration
1	HPrI14A from S. carnosus	[U-15N]	1.7 mM
2	potassium phosphate		20 mM
3	potassium chloride		100 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹H	DSS	protons	internal	direct
¹³C	DSS	carbon	internal	indirect
¹⁵N	DSS	nitrogen	internal	indirect

Referencing offset: -0.63 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹H	DSS	protons	internal	direct
¹³C	DSS	carbon	internal	indirect
¹⁵N	DSS	nitrogen	internal	indirect

Referencing offset: -0.63 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹H	DSS	protons	internal	direct
¹³C	DSS	carbon	internal	indirect
¹⁵N	DSS	nitrogen	internal	indirect

Referencing offset: -0.06 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹H	DSS	protons	internal	direct
¹³C	DSS	carbon	internal	indirect
¹⁵N	DSS	nitrogen	internal	indirect

Referencing offset: -0.16 ppm, Outliers: 4 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 1TXE, Strand ID: A Detail

Release date

2005-01-13

Citation 1

Solution structure of the active-centre mutant I14A of the histidine-containing phosphocarrier protein from Staphylococcus carnosus
Moglich, A., Koch, B., Gronwald, W., Hengstenberg, W., Brunner, E., Kalbitzer, H.
Eur. J. Biochem. (2004), 271, 4815-4824, PubMed 15606769 , DOI 10.1111/j.1432-1033.2004.04447.x , Abstract

Show more 1 citaion

Citation 2

15N and 1H NMR study of histidine containing protein (HPr) from Staphylococcus carnosus at high pressure
Protein Sci. (2000), 9, 693-703, PubMed 10794411 , DOI 10.1110/ps.9.4.693 , Abstract

Hide non-primary 1 citaion

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: P23534 released on 1991-11-01
Title PTHP_STACA Entity Phosphocarrier protein HPr

Related entities 1. HPr monomer, : 1 : 2 : 131 entities Detail

Experiments performed 6 experiments Detail

1 2D-TOCSY

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (800 MHz)

Sample #1

Temperature 298 K, pH 7.14

#	Name	Isotope labeling	Concentration
1	HPrI14A from S. carnosus	[U-15N]	1.7 mM
2	potassium phosphate		20 mM
3	potassium chloride		100 mM

Sample #2

Temperature 298 K, pH 7.14

#	Name	Isotope labeling	Concentration
4	HPrI14A from S. carnosus	[U-13C; U-15N]	1.5 mM
5	potassium phosphate		20 mM
6	potassium chloride		100 mM

2 HCCH-TOCSY

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (800 MHz)

Sample #1

Temperature 298 K, pH 7.14

#	Name	Isotope labeling	Concentration
1	HPrI14A from S. carnosus	[U-15N]	1.7 mM
2	potassium phosphate		20 mM
3	potassium chloride		100 mM

Sample #2

Temperature 298 K, pH 7.14

#	Name	Isotope labeling	Concentration
4	HPrI14A from S. carnosus	[U-13C; U-15N]	1.5 mM
5	potassium phosphate		20 mM
6	potassium chloride		100 mM

3 HSQC

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (800 MHz)

Sample #1

Temperature 298 K, pH 7.14

#	Name	Isotope labeling	Concentration
1	HPrI14A from S. carnosus	[U-15N]	1.7 mM
2	potassium phosphate		20 mM
3	potassium chloride		100 mM

Sample #2

Temperature 298 K, pH 7.14

#	Name	Isotope labeling	Concentration
4	HPrI14A from S. carnosus	[U-13C; U-15N]	1.5 mM
5	potassium phosphate		20 mM
6	potassium chloride		100 mM

4 HNCA

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (800 MHz)

Sample #1

Temperature 298 K, pH 7.14

#	Name	Isotope labeling	Concentration
1	HPrI14A from S. carnosus	[U-15N]	1.7 mM
2	potassium phosphate		20 mM
3	potassium chloride		100 mM

Sample #2

Temperature 298 K, pH 7.14

#	Name	Isotope labeling	Concentration
4	HPrI14A from S. carnosus	[U-13C; U-15N]	1.5 mM
5	potassium phosphate		20 mM
6	potassium chloride		100 mM

5 HNCO

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (800 MHz)

Sample #1

Temperature 298 K, pH 7.14

#	Name	Isotope labeling	Concentration
1	HPrI14A from S. carnosus	[U-15N]	1.7 mM
2	potassium phosphate		20 mM
3	potassium chloride		100 mM

Sample #2

Temperature 298 K, pH 7.14

#	Name	Isotope labeling	Concentration
4	HPrI14A from S. carnosus	[U-13C; U-15N]	1.5 mM
5	potassium phosphate		20 mM
6	potassium chloride		100 mM

6 CBCA(CO)NH

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (800 MHz)

Sample #1

Temperature 298 K, pH 7.14

#	Name	Isotope labeling	Concentration
1	HPrI14A from S. carnosus	[U-15N]	1.7 mM
2	potassium phosphate		20 mM
3	potassium chloride		100 mM

Sample #2

Temperature 298 K, pH 7.14

#	Name	Isotope labeling	Concentration
4	HPrI14A from S. carnosus	[U-13C; U-15N]	1.5 mM
5	potassium phosphate		20 mM
6	potassium chloride		100 mM

nullKeywords NMR structure, mutant protein

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Found 1 Document (1.788 seconds)

BMRB: 6254

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. HPr monomer, : 1 : 2 : 131 entities Detail

Experiments performed 6 experiments Detail

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