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BMRB: 6280

2L3J

Resonance assignment of the double-stranded RNA-binding domains of adenosine deaminase acting on RNA

Authors

Stefl, R., Skrisovska, L., Xu, M., Emeson, R., Allain, F.

Assembly

double-stranded RNA-binding domains of adenosine deaminase acting on RNA

Entity

1. double-stranded RNA-binding domains of adenosine deaminase acting on RNA (polymer, Thiol state: not present), 294 monomers, 31766.33 Da Detail

MQYKLILNGK TLKGETTTEA VDAATAEKVF KQYANDNGVD GEWTYDDATK TFTVTEGSPG PVLPKNALMQ LNEIKPGLQY MLLSQTGPVH APLFVMSVEV NGQVFEGSGP TKKKAKLHAA EKALRSFVQF PNASEAHLAM GRTLSVNTDF TSDQADFPDT LFNGFETPDK SEPPFYVGSN GDDSFSSSGD VSLSASPVPA SLTQPPLPIP PPFPPPSGKN PVMILNELRP GLKYDFLSES GESHAKSFVM SVVVDGQFFE GSGRNKKLAK ARAAQSALAT VFNLHLLEHH HHHH

Formula weight

31766.33 Da

Source organism

Rattus norvegicus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 85.0 %, Completeness: 62.6 %, Completeness (bb): 68.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	62.6 % (2092 of 3344)	65.5 % (1134 of 1732)	54.5 % (718 of 1317)	81.4 % (240 of 295)
Backbone	68.8 % (1179 of 1714)	75.4 % (441 of 585)	59.8 % (514 of 860)	83.3 % (224 of 269)
Sidechain	58.7 % (1116 of 1902)	60.4 % (693 of 1147)	55.8 % (407 of 729)	61.5 % (16 of 26)
Aromatic	28.5 % (81 of 284)	57.0 % (81 of 142)	0.0 % (0 of 141)	0.0 % (0 of 1)
Methyl	73.3 % (217 of 296)	74.3 % (110 of 148)	72.3 % (107 of 148)

1. double-stranded RNA-binding domains of adenosine deaminase acting on RNA

Show sample condition

Sample

Temperature 293 (±0.5) K, pH 8.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	double-stranded RNA-binding domains of adenosine deaminase acting on RNA	[U-85% 2H; U-13C; U-15N]		0.8 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	null	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.42 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	null	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.42 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	null	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.14 ppm, Outliers: 2 Detail

Release date

2005-02-07

Citation

Resonance assignments of the double-stranded RNA-binding domains of adenosine deaminase acting on RNA 2 (ADAR2)
Stefl, R., Skrisovska, L., Xu, M., Emeson, R.B., Allain, F.H.-T.
J. Biomol. NMR (2005), 31, 71-72, PubMed 15692744 , DOI 10.1007/s10858-004-6058-x , Abstract

Related entities 1. double-stranded RNA-binding domains of adenosine deaminase acting on RNA, : 1 entities Detail

Experiments performed 12 experiments Detail

1 [1H,15N]-TROSY