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BMRB: 6291

1X9B

Assignment of hypothetical membrane protein ta0354_69_121

Authors

Wu, B., Yee, A., Huang, Y.H., Ramelot, T., Lemak, A., Kennedy, M., Arrowsmith, C.H.

Assembly

hypothetical membrane protein ta0354 69 121

Entity

1. hypothetical membrane protein ta0354 69 121 (polymer, Thiol state: not present), 53 monomers, 6193.100 Da Detail

RNLSDRAKFE SMINSPSKSV FVRNLNELEA LAVRLGKSYR IQLDQAKEKW KVK

Formula weight

6193.1 Da

Source organism

Thermoplasma acidophilum

Exptl. method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 88.7 %, Completeness: 80.6 %, Completeness (bb): 83.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	80.6 % (533 of 661)	80.1 % (282 of 352)	81.6 % (204 of 250)	79.7 % (47 of 59)
Backbone	83.9 % (265 of 316)	84.0 % (89 of 106)	84.2 % (133 of 158)	82.7 % (43 of 52)
Sidechain	78.6 % (312 of 397)	78.5 % (193 of 246)	79.9 % (115 of 144)	57.1 % (4 of 7)
Aromatic	80.0 % (32 of 40)	95.0 % (19 of 20)	63.2 % (12 of 19)	100.0 % (1 of 1)
Methyl	92.9 % (52 of 56)	92.9 % (26 of 28)	92.9 % (26 of 28)

1. hypothetical membrane protein ta0354

RNLSDRAKFE SMINSPSKSV FVRNLNELEA LAVRLGKSYR IQLDQAKEKW KVK

Show sample condition

Sample

Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	hypothetical membrane protein ta0354	[U-13C; U-15N]		1 mM
2	NaCl			450 mM

Release date

2005-05-25

Citation

Solution structure of hypothetical membrane protein ta0354 from Thermoplasma acidophilum
Wu, B., Yee, A., Huang, Y.H., Ramelot, T., Lemak, A., Kennedy, M., Arrowsmith, C.H.

Related entities 1. hypothetical membrane protein ta0354 69 121, : 1 : 3 entities Detail

Experiments performed 15 experiments Detail

1 15N HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	hypothetical membrane protein ta0354	[U-13C; U-15N]		1 mM
2	NaCl			450 mM

2 HNCO

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	hypothetical membrane protein ta0354	[U-13C; U-15N]		1 mM
2	NaCl			450 mM

3 CBCAcoNH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	hypothetical membrane protein ta0354	[U-13C; U-15N]		1 mM
2	NaCl			450 mM

4 HNCACB

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	hypothetical membrane protein ta0354	[U-13C; U-15N]		1 mM
2	NaCl			450 mM

5 HNCA

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	hypothetical membrane protein ta0354	[U-13C; U-15N]		1 mM
2	NaCl			450 mM

6 HNcoCA

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	hypothetical membrane protein ta0354	[U-13C; U-15N]		1 mM
2	NaCl			450 mM

7 CCTOCSY_NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	hypothetical membrane protein ta0354	[U-13C; U-15N]		1 mM
2	NaCl			450 mM

8 HCCTOCSY_NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	hypothetical membrane protein ta0354	[U-13C; U-15N]		1 mM
2	NaCl			450 mM

9 HNHA

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	hypothetical membrane protein ta0354	[U-13C; U-15N]		1 mM
2	NaCl			450 mM

10 13C HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	hypothetical membrane protein ta0354	[U-13C; U-15N]		1 mM
2	NaCl			450 mM

11 HCCHCOSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	hypothetical membrane protein ta0354	[U-13C; U-15N]		1 mM
2	NaCl			450 mM

12 HCCHTOCSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	hypothetical membrane protein ta0354	[U-13C; U-15N]		1 mM
2	NaCl			450 mM

13 15N NOESY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	hypothetical membrane protein ta0354	[U-13C; U-15N]		1 mM
2	NaCl			450 mM

14 13C NOESY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	hypothetical membrane protein ta0354	[U-13C; U-15N]		1 mM
2	NaCl			450 mM

15 13,13C 4d CCNOESY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	hypothetical membrane protein ta0354	[U-13C; U-15N]		1 mM
2	NaCl			450 mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr6291_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr6291_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	chemical_shift_1	Warning	591		88.7 (chain: 1, length: 53)
1	Chemical shift references	chemical_shift_reference	OK	3		No information

Assigned chemical shifts

Saveframe: chemical_shift_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 53, Sequence coverage: 88.7%
- Total number of assignments
  - ¹³C chemical shifts: 205
  - ¹H chemical shifts: 338
  - ¹⁵N chemical shifts: 48
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Keywords hypothetical membrane protein ta0354_69_121