BMRB: 6312

Solution Structure of Fasciclin-Like Protein From Rhodobacter sphaeroides

Authors

Moody, R.G., Phillips-Jones, M.K., Williamson, M.P.

Assembly

flp monomer

Entity

1. flp monomer (polymer, Thiol state: not present), 137 monomers, 13841.30 Da Detail

ETGDIVETAT GAGSFTTLLT AAEAAGLVDT LKGDGPFTVF APTDAAFAAL PEGTVEDLLK PENKEKLTEI LTYHVVPGEV MSSDLTEGMT AETVEGGALT VTLEGGPKVN GVSISQPDVD ASNGVIHVID GVLMPGA

Formula weight

13841.3 Da

Source organism

Cereibacter sphaeroides

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 98.2 %, Completeness (bb): 99.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	98.2 % (1394 of 1419)	98.3 % (700 of 712)	97.9 % (562 of 574)	99.2 % (132 of 133)
Backbone	99.1 % (799 of 806)	99.3 % (281 of 283)	98.7 % (389 of 394)	100.0 % (129 of 129)
Sidechain	97.5 % (715 of 733)	97.7 % (419 of 429)	97.7 % (293 of 300)	75.0 % (3 of 4)
Aromatic	85.7 % (48 of 56)	85.7 % (24 of 28)	85.7 % (24 of 28)
Methyl	98.0 % (192 of 196)	98.0 % (96 of 98)	98.0 % (96 of 98)

1. flp

ETGDIVETAT GAGSFTTLLT AAEAAGLVDT LKGDGPFTVF APTDAAFAAL PEGTVEDLLK PENKEKLTEI LTYHVVPGEV MSSDLTEGMT AETVEGGALT VTLEGGPKVN GVSISQPDVD ASNGVIHVID GVLMPGA

Show sample condition

Sample

Temperature 298 (±1) K, pH 7 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	flp	[U-13C; U-15N]		1.0 mM
2	sodium phosphate			50 mM

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LACS Plot; CA

Referencing offset: 0.11 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	TSP	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CB

Referencing offset: 0.11 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	TSP	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; HA

Referencing offset: -0.01 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	TSP	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CO

Referencing offset: 0.43 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	TSP	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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Protein Blocks Logo

Calculated from 1 models in PDB: 1W7D, Strand ID: A Detail

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Release date

2006-10-18

Citation

NMR assignment of the Rhodobacter sphaeroides fasciclin-1 domain protein (Fdp)
Moody, R.G., Phillips-Jones, M.K., Williamson, M.P.
Biomol. NMR Assign. (2007), 1, 11-12, PubMed 19636813 , DOI 10.1007/s12104-007-9011-6 , Abstract

Related entities 1. flp monomer, : 1 : 1 : 195 entities Detail

Experiments performed 2 experiments Detail