BMRB: 6376

Complete resonance assignment of the C-terminal domain from TgMIC1

Authors

Saouros, S., Chen, H., Cota, E., Edwards-Jones, B., Simpson, P., Soldati-Favre, D., Matthews, S.

Assembly

TgMIC1-CT

Entity

1. TgMIC1-CT (polymer, Thiol state: all disulfide bound), 137 monomers, 14020.48 Da Detail

KTEIHGDSTK ATLEEGQQLT LTFISTKLDV AVGSCHSLVA NFLDGFLKFQ TGSNSAFDVV EVEEPAGPAV LTIGLGHKGR LAVVLDYTRL NAALGSAAYV VEDSGCSSSE EVSFQGVGSG ATLVVTTLGE SPTAVSA

Formula weight

14020.48 Da

Entity Connection

disulfide 1 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS35:SG	1:CYS106:SG

Source organism

Toxoplasma gondii

Exptl. method

NMR

Refine. method

ARIA

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 92.4 %, Completeness (bb): 98.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	92.4 % (1333 of 1443)	89.6 % (649 of 724)	95.8 % (554 of 578)	92.2 % (130 of 141)
Backbone	98.0 % (800 of 816)	96.2 % (275 of 286)	99.7 % (395 of 396)	97.0 % (130 of 134)
Sidechain	87.4 % (655 of 749)	85.4 % (374 of 438)	92.4 % (281 of 304)	0.0 % (0 of 7)
Aromatic	70.5 % (62 of 88)	70.5 % (31 of 44)	70.5 % (31 of 44)
Methyl	96.3 % (183 of 190)	95.8 % (91 of 95)	96.8 % (92 of 95)

1. TgMIC1-CT

KTEIHGDSTK ATLEEGQQLT LTFISTKLDV AVGSCHSLVA NFLDGFLKFQ TGSNSAFDVV EVEEPAGPAV LTIGLGHKGR LAVVLDYTRL NAALGSAAYV VEDSGCSSSE EVSFQGVGSG ATLVVTTLGE SPTAVSA

Show sample condition

Sample

Temperature 308 (±2) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	TgMIC1-CT	[U-95% 13C; U-95% 15N]		0.5 mM
2	Sodium Chloride			150 mM
3	sodium phosphate			20 mM

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LACS Plot; CA

Referencing offset: -0.72 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.72 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.04 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.71 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 1 models in PDB: 2BVB, Strand ID: A Detail

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Release date

2004-11-03

Citation

Complete resonance assignments of the C-terminal domain from MIC1: a micronemal protein from Toxoplasma gondii
Saouros, S., Chen, H., Simpson, P., Cota, E., Edwards-Jones, B., Soldati-Favre, D., Matthews, S.
J. Biomol. NMR (2005), 31, 177-178, PubMed 15772760 , DOI 10.1007/s10858-004-8237-1 , Abstract

Related entities 1. TgMIC1-CT, : 1 : 1 : 1 entities Detail

Interaction partners 1. TgMIC1-CT, : 1 interactors Detail

Experiments performed 8 experiments Detail