BMRB: 6381

NMR solution structures of mammalian prion proteins

Authors

Calzolai, L., Pioda, G., Lysek, D.A.

Assembly

ovPrP,H168 monomer

Entity

1. ovPrP,H168 monomer (polymer, Thiol state: all disulfide bound), 113 monomers, 13226.54 Da Detail

GSVVGGLGGY MLGSAMSRPL IHFGNDYEDR YYRENMYRYP NQVYYRPVDH YSNQNNFVHD CVNITVKQHT VTTTTKGENF TETDIKIMER VVEQMCITQY QRESQAYYQR GAS

Formula weight

13226.54 Da

Entity Connection

disulfide 1 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS61:SG	1:CYS96:SG

Source organism

Ovis aries

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 88.3 %, Completeness (bb): 83.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	88.3 % (1170 of 1325)	98.0 % (675 of 689)	73.1 % (373 of 510)	96.8 % (122 of 126)
Backbone	83.2 % (559 of 672)	97.4 % (226 of 232)	68.8 % (227 of 330)	96.4 % (106 of 110)
Sidechain	94.2 % (713 of 757)	98.2 % (449 of 457)	87.3 % (248 of 284)	100.0 % (16 of 16)
Aromatic	72.5 % (103 of 142)	93.0 % (66 of 71)	52.1 % (37 of 71)
Methyl	99.0 % (95 of 96)	97.9 % (47 of 48)	100.0 % (48 of 48)

1. sheep prion protein

GSVVGGLGGY MLGSAMSRPL IHFGNDYEDR YYRENMYRYP NQVYYRPVDH YSNQNNFVHD CVNITVKQHT VTTTTKGENF TETDIKIMER VVEQMCITQY QRESQAYYQR GAS

Show sample condition

Sample

Temperature 293 (±0.5) K, pH 4.5 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	sheep prion protein	[U-13C; U-15N]		1.0 mM

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LACS Plot; CA

Referencing offset: 0.22 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.22 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.06 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1XYU, Strand ID: A Detail

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Release date

2004-11-09

Citation

Prion protein NMR structures of cats, dogs, pigs, and sheep
Lysek, D.A., Schorn, C., Nivon, L.G., Esteve-Moya, V., Christen, B., Calzolai, L., von Schroetter, C., Fiorito, F., Herrmann, T., Guntert, P., Wuthrich, K.
Proc. Natl. Acad. Sci. U. S. A. (2005), 102, 640-645, PubMed 15647367 , DOI 10.1073/pnas.0408937102 , Abstract

Related entities 1. ovPrP,H168 monomer, : 1 : 3 : 159 entities Detail

Interaction partners 1. ovPrP,H168 monomer, : 1 interactors Detail

Experiments performed 3 experiments Detail