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BMRB: 6400

2F76

1H, 13C, and 15N Chemical Shift Assignments for the Wild-Type Matrix Protein of Mason-Pfizer Monkey Virus (M-PMV)

Authors

Vlach, J., Lipov, J., Ruml, T., Hrabal, R.

Assembly

wt ma

Entity

1. wt ma (polymer, Thiol state: all free), 100 monomers, 11968.63 Da Detail

MGQELSQHER YVEQLKQALK TRGVKVKYAD LLKFFDFVKD TCPWFPQEGT IDIKRWRRVG DCFQDYYNTF GPEKVPVTAF SYWNLIKELI DKKEVNPQVM

Formula weight

11968.63 Da

Source organism

Mason-Pfizer monkey virus

Exptl. method

NMR

Refine. method

simulated annealing; molecular dynamics; torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 74.9 %, Completeness (bb): 96.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	74.9 % (952 of 1271)	70.1 % (469 of 669)	78.9 % (390 of 494)	86.1 % (93 of 108)
Backbone	96.3 % (568 of 590)	97.5 % (195 of 200)	95.6 % (282 of 295)	95.8 % (91 of 95)
Sidechain	61.6 % (478 of 776)	58.4 % (274 of 469)	68.7 % (202 of 294)	15.4 % (2 of 13)
Aromatic	14.0 % (21 of 150)	28.0 % (21 of 75)	0.0 % (0 of 72)	0.0 % (0 of 3)
Methyl	88.5 % (85 of 96)	87.5 % (42 of 48)	89.6 % (43 of 48)

1. M-PMV WT MA

MGQELSQHER YVEQLKQALK TRGVKVKYAD LLKFFDFVKD TCPWFPQEGT IDIKRWRRVG DCFQDYYNTF GPEKVPVTAF SYWNLIKELI DKKEVNPQVM

Show sample condition

Sample

Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	M-PMV WT MA	[U-13C; U-15N]	1 mM
2	NaH(PO4)2/NaH2PO4		100 mM
3	NaCl		200 mM
4	DTT		10 mM
5	D2O		10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.04 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.04 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.12 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.16 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2F76, Strand ID: X Detail

Release date

2005-07-25

Citation

Assignment of 1H, 13C, and 15N resonances of WT matrix protein and its R55F mutant from Mason-Pfizer monkey virus
Vlach, J., Lipov, J., Veverka, V., Rumlova, M., Ruml, T., Hrabal, R.
J. Biomol. NMR (2005), 31, 381-382, PubMed 15929014 , DOI 10.1007/s10858-005-2473-x , Abstract

Related entities 1. wt ma, : 1 : 2 : 4 : 32 entities Detail

Interaction partners 1. wt ma, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 9 experiments Detail

1 1H-15N HSQC