BMRBj - BMREntryMaster

Found 1 Document (1.548 seconds)

BMRB: 6404

1SZ9

HN, N, CA, CB Chemical Shift Assignments for the CID domain of Pcf11

Authors

Hollingworth, D., Frenkiel, T.A., Kelly, G., Noble, C.G., Taylor, I.A., Ramos, A.

Assembly

Pcf11 CID

Entity

1. Pcf11 CID (polymer, Thiol state: all free), 142 monomers, 16472.86 Da Detail

MDHDTEVIVK DFNSILEELT FNSRPIITTL TKLAEENISC AQYFVDAIES RIEKCMPKQK LYAFYALDSI CKNVGSPYTI YFSRNLFNLY KRTYLLVDNT TRTKLINMFK LWLNPNDTGL PLFEGSALEK IEQFLIKASA LH

Formula weight

16472.86 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.7 %, Completeness: 33.5 %, Completeness (bb): 63.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	33.5 % (583 of 1741)	20.1 % (181 of 902)	39.4 % (271 of 688)	86.8 % (131 of 151)
Backbone	63.7 % (536 of 842)	51.1 % (144 of 282)	61.9 % (262 of 423)	94.9 % (130 of 137)
Sidechain	16.7 % (173 of 1038)	6.0 % (37 of 620)	33.4 % (135 of 404)	7.1 % (1 of 14)
Aromatic	3.6 % (6 of 166)	3.6 % (3 of 83)	2.4 % (2 of 82)	100.0 % (1 of 1)
Methyl	10.1 % (18 of 178)	7.9 % (7 of 89)	12.4 % (11 of 89)

1. Pcf11 CID

MDHDTEVIVK DFNSILEELT FNSRPIITTL TKLAEENISC AQYFVDAIES RIEKCMPKQK LYAFYALDSI CKNVGSPYTI YFSRNLFNLY KRTYLLVDNT TRTKLINMFK LWLNPNDTGL PLFEGSALEK IEQFLIKASA LH

Show sample condition

Sample

Temperature 300 (±1) K, pH 7.4 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	Pcf11 CID	[U-13C; U-15N]		0.8 mM

Release date

2005-07-25

Citation

Backbone assignment of PCF11 CTD binding domain
Hollingworth, D., Kelly, G., Frenkiel, T.A., Noble, C.G., Taylor, I.A., Ramos, A.
J. Biomol. NMR (2005), 31, 363-363, PubMed 15929005 , DOI 10.1007/s10858-005-1392-1 , Abstract

Related entities 1. Pcf11 CID, : 1 : 2 : 2 : 23 entities Detail

Interaction partners 1. Pcf11 CID, : 70 interactors Detail

More details for 70 interactors identified by 4 citations

Experiments performed 5 experiments Detail

#	Name	Isotope labeling	Type	Concentration
1	Pcf11 CID	[U-13C; U-15N; U-2H]		0.8 mM

#	Name	Isotope labeling	Type	Concentration
3	Pcf11 CID	[U-15N]		0.8 mM

#	Name	Isotope labeling	Type	Concentration
1	Pcf11 CID	[U-13C; U-15N; U-2H]		0.8 mM

#	Name	Isotope labeling	Type	Concentration
3	Pcf11 CID	[U-15N]		0.8 mM

#	Name	Isotope labeling	Type	Concentration
1	Pcf11 CID	[U-13C; U-15N; U-2H]		0.8 mM

#	Name	Isotope labeling	Type	Concentration
3	Pcf11 CID	[U-15N]		0.8 mM

#	Name	Isotope labeling	Type	Concentration
1	Pcf11 CID	[U-13C; U-15N; U-2H]		0.8 mM

#	Name	Isotope labeling	Type	Concentration
3	Pcf11 CID	[U-15N]		0.8 mM

#	Name	Isotope labeling	Type	Concentration
1	Pcf11 CID	[U-13C; U-15N; U-2H]		0.8 mM

#	Name	Isotope labeling	Type	Concentration
3	Pcf11 CID	[U-15N]		0.8 mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr6404_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr6404_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	chem_shift_list_1	Warning	518		93.0 (chain: 1, length: 142)
1	Chemical shift references	chemical_shift_referencing	OK	3		No information

Assigned chemical shifts

Saveframe: chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 142, Sequence coverage: 93.0%
- Total number of assignments
  - ¹H chemical shifts: 130
  - ¹⁵N chemical shifts: 130
  - ¹³C chemical shifts: 258
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	902	130	14.4
¹⁵N chemical shifts	156	130	83.3
¹³C chemical shifts	688	258	37.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	282	130	46.1
¹⁵N chemical shifts	137	130	94.9
¹³C chemical shifts	284	132	46.5

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Found 1 Document (1.548 seconds)

BMRB: 6404

Sample

Related entities 1. Pcf11 CID, : 1 : 2 : 2 : 23 entities Detail

Interaction partners 1. Pcf11 CID, : 70 interactors Detail

Experiments performed 5 experiments Detail

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Chemical shift validation 3 contents Detail