BMRB: 6407

1H, 15N resonance assignment of the C-terminal domain of Human Papillomavirus Oncoprotein E6

Authors

Nomine, Y., Trave, G., Kieffer, B.

Assembly

HPV16 E6 C-terminal domain

Entity

1. C terminal domain of E6 (polymer, Thiol state: all other bound), 75 monomers, 8888.091 Da Detail

GAMSYSLYGT TLEQQYNKPL SDLLIRCINC QKPLSPEEKQ RHLDKKQRFH NIRGRWTGRC MSCSRSSRTR RETQL

2. ZN (non-polymer), 65.409 Da

Total weight

8953.5 Da

Max. entity weight

8888.091 Da

Entity Connection

na 4 Detail

---

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS27:SG	2:ZN1:ZN
2	na	sing	1:CYS30:SG	2:ZN1:ZN
3	na	sing	1:CYS60:SG	2:ZN1:ZN
4	na	sing	1:CYS63:SG	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

simulated annealing, protocol refine.inp

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.3 %, Completeness: 94.2 %, Completeness (bb): 95.5 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	15N
All	94.2 % (537 of 570)	94.1 % (459 of 488)	95.1 % (78 of 82)
Backbone	95.5 % (213 of 223)	96.0 % (145 of 151)	94.4 % (68 of 72)
Sidechain	93.4 % (324 of 347)	93.2 % (314 of 337)	100.0 % (10 of 10)
Aromatic	92.9 % (26 of 28)	92.6 % (25 of 27)	100.0 % (1 of 1)
Methyl	92.9 % (26 of 28)	92.9 % (26 of 28)

1. C terminal domain of E6

GAMSYSLYGT TLEQQYNKPL SDLLIRCINC QKPLSPEEKQ RHLDKKQRFH NIRGRWTGRC MSCSRSSRTR RETQL

Show sample condition

Sample

Temperature 288 (±0.1) K, pH 6.8 (±0.1), Details The E6-C 4C/4S construct corresponds to residues 77-151 of HPV16 E6 with four non-conserved cystein residues (80,97,111 and 140) mutated into serines. At the N-terminus of the construct there are three additional residues (GAM) due to TEV cleavage.

#	Name	Isotope labeling	Type	Concentration
1	C terminal domain of E6	[U-15N]		0.3 ~ 1.2 mM
2	Tris-HCl deuterated			20 mM
3	NaCl			50 mM
4	dithiothreitol			1 mM
5	deuterium oxide			10 %

Hide sample codition

Protein Blocks Logo

Calculated from 10 models in PDB: 2FK4, Strand ID: A Detail

---

Release date

2005-03-17

Citation

1H and 15N resonance assignment, secondary structure and dynamic behaviour of the C-terminal domain of human papillomavirus oncoprotein E6
Nomine, Y., Charbonnier, S., Miguet, L., Potier, N., Van Dorsselaer, A., Atkinson, R.ANDREW., Trave, G., Kieffer, B.
J. Biomol. NMR (2005), 31, 129-141, PubMed 15772752 , DOI 10.1007/s10858-004-7802-y , Abstract

Related entities 1. C terminal domain of E6, : 1 : 1 : 6 : 43 entities Detail

Interaction partners 1. C terminal domain of E6, : 33 interactors Detail

Experiments performed 3 experiments Detail