BMRB: 6412

FRAGMENT 33-61 OF BOVINE alpha-HEMOGLBIN: THE EFFECT OF C-TERMINAL AMIDATION AND IDENTIFICATION OF THE MINIMAL PORTION WITH ANTIFUNGAL ACTIVITY

Authors

Machado, A., Sforca, M.L., Miranda, A., Daffre, S., Spisni, A., Pertinhez, T.A., Miranda, M.M.

Assembly

fragment 48-61of bovine alpha-hemoglobin

Entity

1. fragment 48-61of bovine alpha-hemoglobin (polymer, Thiol state: not present), 14 monomers, 1376.520 Da Detail

LSHGSAQVKG HGAK

Formula weight

1376.52 Da

Source organism

Bos taurus

Exptl. method

NMR

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 93.3 %, Completeness (bb): 96.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	93.3 % (70 of 75)	93.3 % (70 of 75)
Backbone	96.8 % (30 of 31)	96.8 % (30 of 31)
Sidechain	90.9 % (40 of 44)	90.9 % (40 of 44)
Aromatic	100.0 % (4 of 4)	100.0 % (4 of 4)
Methyl	83.3 % (5 of 6)	83.3 % (5 of 6)

1. 48-61 fragment

LSHGSAQVKG HGAK

Show sample condition

Sample

Temperature 310 (±1) K, pH 4.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	48-61 fragment			1.0 mM
2	sodium dodecyl sulfate (SDS)			200 mM

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Coupling constant

13 J values in 1 lists
Temperature 310 (±1) K, pH 4.0 (±0.2) Detail

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Temperature 310 (±1) K, pH 4.0 (±0.2)

List #1 J_values_set_1

Release date

2005-10-19

Citation 1

The micelle-bound structure of an antimicrobial peptide derived from the alpha-chain of bovine hemoglobin isolated from the tick Boophilus microplus
Sforca, M.L., Machado, A., Figueredo, R.C., Oyama, S., Silva, F.D., Miranda, A., Daffre, S., Miranda, M.TERESA., Spisni, A., Pertinhez, T.A.
Biochemistry (2005), 44, 6440-6451, PubMed 15850378 , DOI 10.1021/bi0475323 , Abstract

Show more 1 citaion

Citation 2

NMRPipe: a multidimensional spectral processing system based on UNIX pipes
Delaglio, F., Grzesiek, S., Vuister, G.W., Zhu, G., Pfeifer, J., Bax, A.
J. Biomol. NMR (1995), 6, 277-293, PubMed 8520220 , Abstract

Hide non-primary 1 citaion

Related entities 1. fragment 48-61of bovine alpha-hemoglobin, : 1 : 20 : 1 : 158 : 22 entities Detail

Interaction partners 1. fragment 48-61of bovine alpha-hemoglobin, : 32 interactors Detail

Experiments performed 4 experiments Detail

Chemical shift validation 3 contents Detail