BMRB: 6415

Three-disulfide variant of hen lysozyme: C30A/C115A

Authors

Yokota, A., Hirai, K., Miyauchi, H., Iimura, S., Noda, Y., Inoue, K., Akasaka, K., Tachibana, H., Segawa, S.

Assembly

hen egg white lysozyme

Entity

1. hen egg white lysozyme (polymer, Thiol state: all disulfide bound), 130 monomers, 14380.07 Da Detail

MKVFGRCELA AAMKRHGLDN YRGYSLGNWV AAAKFESNFN TQATNRNTDG STDYGILQIN SRWWCNDGRT PGSRNLCNIP CSALLSSDIT ASVNCAKKIV SDGNGMNAWV AWRNRAKGTD VQAWIRGCRL

Formula weight

14380.07 Da

Entity Connection

disulfide 3 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS7:SG	1:CYS128:SG
2	disulfide	sing	1:CYS65:SG	1:CYS81:SG
3	disulfide	sing	1:CYS77:SG	1:CYS95:SG

Source organism

Gallus gallus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.9 %, Completeness: 84.4 %, Completeness (bb): 94.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	84.4 % (639 of 757)	84.4 % (639 of 757)
Backbone	94.4 % (255 of 270)	94.4 % (255 of 270)
Sidechain	78.9 % (384 of 487)	78.9 % (384 of 487)
Aromatic	90.8 % (59 of 65)	90.8 % (59 of 65)
Methyl	95.1 % (58 of 61)	95.1 % (58 of 61)

1. hen egg white lysozyme variant

MKVFGRCELA AAMKRHGLDN YRGYSLGNWV AAAKFESNFN TQATNRNTDG STDYGILQIN SRWWCNDGRT PGSRNLCNIP CSALLSSDIT ASVNCAKKIV SDGNGMNAWV AWRNRAKGTD VQAWIRGCRL

Show sample condition

Sample

Temperature 298 (±0.1) K, pH 3.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	hen egg white lysozyme variant	[U-15N]		0.2 ~ 1.0 mM

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Release date

2005-04-03

Citation 1

NMR characterization of three-disulfide variants of lysozyme, C64A/C80A, C76A/C94A, and C30A/C115A--a marginally stable state in folded proteins
Yokota, A., Hirai, K., Miyauchi, H., Iimura, S., Noda, Y., Inoue, K., Akasaka, K., Tachibana, H., Segawa, S.
Biochemistry (2004), 43, 6663-6669, PubMed 15157100 , DOI 10.1021/bi049967w , Abstract

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Citation 2

Characterisation of the dominant oxidative folding intermediate of hen lysozyme
J. Mol. Biol. (1999), 290, 781-796, PubMed 10395829 , DOI 10.1006/jmbi.1999.2915 , Abstract

Citation 3

NMR structural study of two-disulfide variant of hen lysozyme: 2SS[6-127, 30-115]--a disulfide intermediate with a partly unfolded structure
Biochemistry (2002), 41, 2130-2139, PubMed 11841203 , Abstract

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Related entities 1. hen egg white lysozyme, : 1 : 105 : 141 entities Detail

Interaction partners 1. hen egg white lysozyme, : 7 interactors Detail

Experiments performed 7 experiments Detail

Chemical shift validation 3 contents Detail