BMRB: 6433

Sequence specific 1HN, 13C and 15N resonance assignments of a novel calcium binding protein from Entamoeba histolytica

Authors

Bansal-Mutalik, R., Mustafi, S.MITRA., Bhattacharya, A., Chary, V.KANDALA.

Assembly

Entamoeba histolytica calcium binding protein 2

Entity

1. Entamoeba histolytica calcium binding protein (polymer, Thiol state: not present), 134 monomers, 14943.81 Da Detail

MAEALFKQLD ANGDGSVSYE EVKAFVSSKR PIKNEQLLQL IFKAIDIDGN GEIDLAEFTK FAAAVKEQDL SDEKVGLKIL YKLMDADGDG KLTKEEVTTF FKKFGYEKVV DQIMKADANG DGYITLEEFL AFNL

2. CA (non-polymer), 40.078 Da

Total weight

14983.888 Da

Max. entity weight

14943.81 Da

Source organism

Escherichia coli

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.3 %, Completeness: 64.6 %, Completeness (bb): 91.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	64.6 % (1024 of 1586)	61.8 % (508 of 822)	63.1 % (392 of 621)	86.7 % (124 of 143)
Backbone	91.8 % (736 of 802)	86.3 % (239 of 277)	95.2 % (373 of 392)	93.2 % (124 of 133)
Sidechain	44.6 % (405 of 908)	49.4 % (269 of 545)	38.5 % (136 of 353)	0.0 % (0 of 10)
Aromatic	18.2 % (24 of 132)	25.8 % (17 of 66)	10.6 % (7 of 66)
Methyl	44.2 % (69 of 156)	67.9 % (53 of 78)	20.5 % (16 of 78)

1. Entamoeba histolytica calcium binding protein

MAEALFKQLD ANGDGSVSYE EVKAFVSSKR PIKNEQLLQL IFKAIDIDGN GEIDLAEFTK FAAAVKEQDL SDEKVGLKIL YKLMDADGDG KLTKEEVTTF FKKFGYEKVV DQIMKADANG DGYITLEEFL AFNL

Show sample condition

Sample

Temperature 308 (±0.2) K, pH 4.2 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Entamoeba histolytica calcium binding protein	[U-13C; U-15N]		1.5 mM

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LACS Plot; CA

Referencing offset: 1.26 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CB

Referencing offset: 1.26 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; HA

Referencing offset: 0.04 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CO

Referencing offset: -0.43 ppm, Outliers: 4 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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Release date

2005-07-25

Citation

Sequence specific 1HN, 13C and 15N resonance assignments of a novel calcium-binding protein from Entamoeba histolytica
Bansal-Mutalik, R., Mustafi, S.MITRA., Bhattacharya, A., Chary, K.V.R.
J. Biomol. NMR (2005), 31, 379-380, PubMed 15929013 , DOI 10.1007/s10858-005-2471-z , Abstract

Related entities 1. Entamoeba histolytica calcium binding protein, : 1 : 299 entities Detail

Experiments performed 6 experiments Detail

Chemical shift validation 4 contents Detail