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BMRB: 6479

1Y6U

The Structure of the Excisionase (Xis) Protein from Conjugative Transposon Tn916 Provides Insights into the Regulation of Heterobivalent Tyrosine Recombinases

Authors

Abbani, M.A., Iwahara, M., Clubb, R.T.

Assembly

Excisionase from transposon Tn916

Entity

1. Excisionase from transposon Tn916 (polymer, Thiol state: not present), 70 monomers, 8375.556 Da Detail

AGHMKQTDIP IWERYTLTIE EASKYFRIGE NKLRRLAEEN KNANWLIMNG NRIQIKRKQF EKIIDTLDAI

Formula weight

8375.556 Da

Source organism

Enterococcus faecalis

Exptl. method

NMR

Refine. method

Hybrid distance geometry and simulated annealing calculations

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 95.7 %, Completeness: 86.0 %, Completeness (bb): 92.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	86.0 % (759 of 883)	81.0 % (379 of 468)	91.3 % (306 of 335)	92.5 % (74 of 80)
Backbone	92.8 % (388 of 418)	91.5 % (130 of 142)	94.2 % (195 of 207)	91.3 % (63 of 69)
Sidechain	81.8 % (435 of 532)	76.4 % (249 of 326)	89.7 % (175 of 195)	100.0 % (11 of 11)
Aromatic	68.8 % (44 of 64)	68.8 % (22 of 32)	66.7 % (20 of 30)	100.0 % (2 of 2)
Methyl	87.2 % (68 of 78)	87.2 % (34 of 39)	87.2 % (34 of 39)

1. Excisionase from transposon Tn916

AGHMKQTDIP IWERYTLTIE EASKYFRIGE NKLRRLAEEN KNANWLIMNG NRIQIKRKQF EKIIDTLDAI

Show sample condition

Sample

Pressure 1 atm, Temperature 303 K, pH 4.5

#	Name	Isotope labeling	Concentration
1	Excisionase from transposon Tn916	[U-13C; U-15N]	1 mM
2	NaOAc		30 mM
3	NaCl		60 mM
4	H2O		93 %
5	D2O		7 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	null	direct	1.0
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.25 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	null	direct	1.0
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.25 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	null	direct	1.0
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 5 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	null	direct	1.0
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.04 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1Y6U, Strand ID: A Detail

Coupling constant

32 J values in 1 lists
Pressure 1 atm, Temperature 303 K, pH 4.5 Detail

Release date

2005-04-03

Citation 1

The structure of the excisionase (Xis) protein from conjugative transposon Tn916 provides insights into the regulation of heterobivalent tyrosine recombinases
Abbani, M.A., Iwahara, M., Clubb, R.T.
J. Mol. Biol. (2005), 347, 11-25, PubMed 15733914 , DOI 10.1016/j.jmb.2005.01.019 , Abstract

Show more 1 citaion

Citation 2

1H, 13C and 15N chemical shift referencing in biomolecular NMR
Wishart, D.S., Bigam, C.G., Yao, J., Abildgaard, F., Dyson, H.J., Oldfield, E., Markley, J.L., Sykes, B.D.
J. Biomol. NMR (1995), 6, 135-140, PubMed 8589602 , DOI: , Abstract

Hide non-primary 1 citaion

Related entities 1. Excisionase from transposon Tn916, : 1 : 1 : 6 entities Detail

Experiments performed 16 experiments Detail

1 2D 1H-13C CT-HSQC