BMRB: 6524

ApoE N-terminal domain

Authors

Xu, C., Sivashanmugam, A., Hoyt, D., Wang, J.

Assembly

apoE N-terminal domain

Entity

1. apoE N-terminal domain (polymer, Thiol state: all free), 183 monomers, 21191.71 Da Detail

KVEQAVETEP EPELRQQTEW QSGQRWELAL GRFWDYLRWV QTLSEQVQEE LLSSQVTQEL RALMDETMKE LKAYKSELEE QLTPVAEETR ARLSKELQAA QARLGADMED VCGRLVQYRG EVQAMLGQST EELRVRLASH LRKLRKRLLR DADDLQKRLA VYQAGAREGA ERGLSAIRER LGP

Formula weight

21191.71 Da

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.5 %, Completeness: 37.6 %, Completeness (bb): 68.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	37.6 % (817 of 2170)	22.0 % (252 of 1147)	47.3 % (389 of 822)	87.6 % (176 of 201)
Backbone	68.1 % (742 of 1090)	52.2 % (194 of 372)	69.0 % (372 of 539)	98.3 % (176 of 179)
Sidechain	19.4 % (243 of 1253)	7.5 % (58 of 775)	40.6 % (185 of 456)	0.0 % (0 of 22)
Aromatic	4.3 % (4 of 94)	8.5 % (4 of 47)	0.0 % (0 of 43)	0.0 % (0 of 4)
Methyl	16.3 % (34 of 208)	9.6 % (10 of 104)	23.1 % (24 of 104)

1. human apolipoprotein E N-terminal domain

KVEQAVETEP EPELRQQTEW QSGQRWELAL GRFWDYLRWV QTLSEQVQEE LLSSQVTQEL RALMDETMKE LKAYKSELEE QLTPVAEETR ARLSKELQAA QARLGADMED VCGRLVQYRG EVQAMLGQST EELRVRLASH LRKLRKRLLR DADDLQKRLA VYQAGAREGA ERGLSAIRER LGP

Show sample condition

Sample #1

Temperature 303 (±0.1) K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	apoE N-terminal domain	[U-13C; U-15N; U-70% 2H]	protein	1 mM
2	Sodium Phosphate		buffer	100 mM
3	NaN3		antimicrobial agent	1 mM
4	DSS		internal reference	0.05 mM
5	EDTA		chelating agent	10 mM
6	d-DTT		reducing agent	90 mM

Sample #2

Temperature 303 (±0.1) K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
7	apoE N-terminal domain	[U-13C; U-15N; U-35% 2H]	protein	1 mM
8	Sodium Phosphate		buffer	100 mM
9	NaN3		antimicrobial agent	1 mM
10	DSS		internal reference	0.06 mM
11	EDTA		chelating agent	10 mM
12	d-DTT		reducing agent	80 mM

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LACS Plot; CA

Referencing offset: 0.34 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.34 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Release date

2005-07-25

Citation

A complete backbone assignment of the apolipoprotein E LDL receptor binding domain
Xu, C., Sivashanmugam, A., Hoyt, D., Wang, J.
J. Biomol. NMR (2005), 32, 177-177, PubMed 16034672 , DOI 10.1007/s10858-005-6729-2 , Abstract

Related entities 1. apoE N-terminal domain, : 1 : 4 : 2 : 13 : 77 entities Detail

Interaction partners 1. apoE N-terminal domain, : 39 interactors Detail

Experiments performed 5 experiments Detail

Chemical shift validation 3 contents Detail