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1H, 13C, and 15N Chemical Shift Assignments for the DNA binding domain of STPA from E. coli

Authors

Ono, S., Ladbury, J.E., Williams, M.A.

Assembly

DNA binding domain of STPA

Entity

1. DNA binding domain of STPA (polymer, Thiol state: not present), 44 monomers, 4875.448 Da Detail

QPRPAKYKFT DVNGETKTWT GQGRTPKPIA QALAEGKSLD DFLI

Formula weight

4875.448 Da

Source organism

Escherichia coli

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 95.8 %, Completeness (bb): 94.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	95.8 % (498 of 520)	96.4 % (264 of 274)	94.5 % (190 of 201)	97.8 % (44 of 45)
Backbone	94.9 % (243 of 256)	96.6 % (85 of 88)	93.0 % (119 of 128)	97.5 % (39 of 40)
Sidechain	96.7 % (294 of 304)	96.2 % (179 of 186)	97.3 % (110 of 113)	100.0 % (5 of 5)
Aromatic	100.0 % (40 of 40)	100.0 % (20 of 20)	100.0 % (19 of 19)	100.0 % (1 of 1)
Methyl	92.9 % (39 of 42)	90.5 % (19 of 21)	95.2 % (20 of 21)

1. DNA binding domain of STPA

QPRPAKYKFT DVNGETKTWT GQGRTPKPIA QALAEGKSLD DFLI

Show sample condition

Sample

Temperature 298 (±1) K

#	Name	Isotope labeling	Concentration
1	DNA binding domain of STPA	[U-98% 13C; U-98% 15N]	1.5 (±0.1) mM
2	sodium chloride		100 (±5.0) mM
3	sodium phosphate		10 (±0.5) mM
4	EDTA		0.1 (±0.05) mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.25 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.25 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.11 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -1.04 ppm, Outliers: 2 Detail

Release date

2006-02-22

Citation 1

Characterization of the bacterial nucleoid protein STPA: Structure, self-association and interaction with H-NS
Ono, S., Leonard, P.G., Williams, M.A., Ladbury, J.E.

Show more 3 citaions

Citation 2

Nucleotide sequence of a newly-identified Escherichia coli gene, stpA, encoding an H-NS-like protein
Zhang, A., Belfort, M.
Nucleic Acids Res. (1992), 20, 6735-6735, PubMed 1480493 , DOI: , Abstract

Citation 3

NMRPipe: a multidimensional spectral processing system based on UNIX pipes
Delaglio, F.
J. Biomol. NMR (1995), 6, 277-293, PubMed 8520220 , Abstract

Citation 4

Solution structure and dynamics of ras p21.GDP determined by heteronuclear three- and four-dimensional NMR spectroscopy
Kraulis, P.J.
Biochemistry (1994), 33, 3515-3531, PubMed 8142349 , Abstract

Hide non-primary 3 citaions

Related entities 1. DNA binding domain of STPA, : 1 : 2 : 19 entities Detail

Experiments performed 11 experiments Detail

1 1H15N_HSQC