BMRBj - BMREntryMaster

Found 1 Document (1.506 seconds)

BMRB: 6546

7OVC

1H, 13C, and 15N Chemical Shift Assignments for Human Protein CGI-126(HSPC155): the Northeast Structural Genomics Consortium Target HR41

Authors

Liu, G., Szyperski, T.

Assembly

CGI-126 monomer

Entity

1. CGI-126 monomer (polymer, Thiol state: all free), 175 monomers, 20523.25 Da Detail

LEHHHHHHMA DEATRRVVSE IPVLKTNAGP RDRELWVQRL KEEYQSLIRY VENNKNADND WFRLESNKEG TRWFGKCWYI HDLLKYEFDI EFDIPITYPT TAPEIAVPEL DGKTAKMYRG GKICLTDHFK PLWARNVPKF GLAHLMALGL GPWLAVEIPD LIQKGVIQHK EKCNQ

Formula weight

20523.25 Da

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.4 %, Completeness: 86.7 %, Completeness (bb): 81.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	86.7 % (1858 of 2142)	91.6 % (1027 of 1121)	78.5 % (657 of 837)	94.6 % (174 of 184)
Backbone	81.2 % (836 of 1030)	92.6 % (324 of 350)	69.3 % (357 of 515)	93.9 % (155 of 165)
Sidechain	92.2 % (1178 of 1277)	91.2 % (703 of 771)	93.6 % (456 of 487)	100.0 % (19 of 19)
Aromatic	87.3 % (192 of 220)	87.3 % (96 of 110)	86.5 % (90 of 104)	100.0 % (6 of 6)
Methyl	98.4 % (183 of 186)	97.8 % (91 of 93)	98.9 % (92 of 93)

1. human protein CGI-126: NESGC target HR41

LEHHHHHHMA DEATRRVVSE IPVLKTNAGP RDRELWVQRL KEEYQSLIRY VENNKNADND WFRLESNKEG TRWFGKCWYI HDLLKYEFDI EFDIPITYPT TAPEIAVPEL DGKTAKMYRG GKICLTDHFK PLWARNVPKF GLAHLMALGL GPWLAVEIPD LIQKGVIQHK EKCNQ

Show sample condition

Sample

Temperature 298 K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	human protein CGI-126	[U-13C; U-15N]		1.0 mM

Release date

2005-08-31

Citation

GFT NMR based resonance assignment for the 21 kDa human protein UFC1
Liu, G., Aramini, J., Atreya, H.S., Eletski, A., Xiao, R., Acton, T.B., Ma, L., Montelione, G.T., Szyperski, T.
J. Biomol. NMR (2005), 32, 261-261, PubMed 16132835 , DOI 10.1007/s10858-005-7941-9 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: Q9Y3C8 released on 2000-05-30
Title UFC1_HUMAN Entity Ubiquitin-fold modifier-conjugating enzyme 1

Related entities 1. CGI-126 monomer, : 1 : 10 : 43 entities Detail

Interaction partners 1. CGI-126 monomer, : 6 interactors Detail

More details for 6 interactors identified by 5 citations

Experiments performed 5 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr6546_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr6546_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	chem_shift_list_1	Warning	2007		94.3 (chain: 1, length: 175)
1	Chemical shift references	chemical_shift_referencing	OK	3		No information

Assigned chemical shifts

Saveframe: chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 175, Sequence coverage: 94.3%
- Total number of assignments
  - ¹H chemical shifts: 1221
  - ¹³C chemical shifts: 615
  - ¹⁵N chemical shifts: 171
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	350	326	93.1
¹³C chemical shifts	350	164	46.9
¹⁵N chemical shifts	165	152	92.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	1121	1038	92.6
¹³C chemical shifts	837	615	73.5
¹⁵N chemical shifts	194	171	88.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	771	712	92.3
¹³C chemical shifts	487	451	92.6
¹⁵N chemical shifts	29	19	65.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	110	96	87.3
¹³C chemical shifts	104	90	86.5
¹⁵N chemical shifts	6	6	100.0

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Found 1 Document (1.506 seconds)

BMRB: 6546

Sample

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. CGI-126 monomer, : 1 : 10 : 43 entities Detail

Interaction partners 1. CGI-126 monomer, : 6 interactors Detail

Experiments performed 5 experiments Detail

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Chemical shift validation 3 contents Detail