1H, 13C, and 15N NMR Sequence-specific Resonance Assignments for Rat Ca2+-S100A1
MGSELETAME TLINVFHAHS GKEGDKYKLS KKELKDLLQT ELSSFKDVQK DADAVDKIMK ELDENGDGEV DFQEFVVLVA ALTVACNNFF WENS
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 79.5 % (870 of 1094) | 85.1 % (481 of 565) | 70.0 % (298 of 426) | 88.3 % (91 of 103) |
Backbone | 83.3 % (470 of 564) | 95.3 % (184 of 193) | 70.4 % (195 of 277) | 96.8 % (91 of 94) |
Sidechain | 79.0 % (489 of 619) | 79.8 % (297 of 372) | 80.7 % (192 of 238) | 0.0 % (0 of 9) |
Aromatic | 11.4 % (10 of 88) | 13.6 % (6 of 44) | 9.3 % (4 of 43) | 0.0 % (0 of 1) |
Methyl | 95.1 % (97 of 102) | 94.1 % (48 of 51) | 96.1 % (49 of 51) |
1. S100A1
MGSELETAME TLINVFHAHS GKEGDKYKLS KKELKDLLQT ELSSFKDVQK DADAVDKIMK ELDENGDGEV DFQEFVVLVA ALTVACNNFF WENSTemperature 310 K, pH 7.2 (±0), Details 0.5-3 mM S100A1, either 15N labeled or 13C, 15N labeled, prepared in H2O with 10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | S100A1 | [U-13C; U-15N] | 0.5 ~ 3.0 mM | |
2 | Calcium | 0.0 ~ 0.0 mM | ||
3 | d11-Tris | 20.0 ~ 40.0 mM | ||
4 | CaCl2 | 20 mM | ||
5 | NaN3 | 0.35 mM | ||
6 | NaCl | 15.0 ~ 18.0 mM | ||
7 | DTT | 20.0 ~ 35.0 mM | ||
8 | D20 | 10 % | ||
9 | H20 | 90 % |
Temperature 310 K, pH 7.2 (±0), Details 0.5-3 mM S100A1, either 15N labeled or 13C, 15N labeled, prepared in H2O with 10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
10 | S100A1 | [U-15N] | 0.5 ~ 3.0 mM | |
11 | Calcium | 0.0 ~ 0.0 mM | ||
12 | d11-Tris | 20.0 ~ 40.0 mM | ||
13 | CaCl2 | 20 mM | ||
14 | NaN3 | 0.35 mM | ||
15 | NaCl | 15.0 ~ 18.0 mM | ||
16 | DTT | 20.0 ~ 35.0 mM | ||
17 | D20 | 10 % | ||
18 | H20 | 90 % |