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Found 1 Document (1.826 seconds)

BMRB: 6624

2BYE

Backbone 1H, 13C, and 15N Resonance Assignments for the 13kD Ras Association Domain 1 (RA1) from Phospholipase C (epsilon)

Authors

Harris, R., Bunney, T.D., Katan, M., Driscoll, P.C.

Assembly

RA1

Entity

1. RA1 (polymer, Thiol state: all free), 110 monomers, 12788.47 Da Detail

GEERKCLQTH RVTVHGVPGP EPFTVFTING GTKAKQLLQQ ILTNEQDIKP VTTDYFLMEE KYFISKEKNE CRKQPFQRAI GPEEEIMQIL SSWFPEEGYM GRIVLKTQQE

Formula weight

12788.47 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

RESTRAINED SIMULATED ANNEALING, WATER REFINEMENT

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.5 %, Completeness: 75.6 %, Completeness (bb): 82.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	75.6 % (1023 of 1353)	79.1 % (566 of 716)	70.0 % (364 of 520)	79.5 % (93 of 117)
Backbone	82.5 % (533 of 646)	86.4 % (191 of 221)	78.9 % (254 of 322)	85.4 % (88 of 103)
Sidechain	70.2 % (568 of 809)	75.8 % (375 of 495)	62.7 % (188 of 300)	35.7 % (5 of 14)
Aromatic	14.4 % (15 of 104)	26.9 % (14 of 52)	0.0 % (0 of 51)	100.0 % (1 of 1)
Methyl	96.2 % (102 of 106)	96.2 % (51 of 53)	96.2 % (51 of 53)

1. ra1

GEERKCLQTH RVTVHGVPGP EPFTVFTING GTKAKQLLQQ ILTNEQDIKP VTTDYFLMEE KYFISKEKNE CRKQPFQRAI GPEEEIMQIL SSWFPEEGYM GRIVLKTQQE

Show sample condition

Sample #1

Temperature 298 K, pH 7.0 (±0.2), Details 15N labelled RA1

#	Name	Isotope labeling	Type	Concentration
1	ra1	[U-15N]	protein	1 mM
2	sodium chloride		salt	250 mM
3	DTT		buffer	5 mM
4	TRIS		buffer	25 mM
5	EDTA		buffer	0.1 mM

Sample #2

Temperature 298 K, pH 7.0 (±0.2), Details 13C/15N labelled RA1

#	Name	Isotope labeling	Type	Concentration
6	ra1	[U-13C; U-15N]	protein	1 mM
7	sodium chloride		salt	250 mM
8	DTT		buffer	5 mM
9	TRIS		buffer	25 mM
10	EDTA		buffer	0.1 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.1 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.1 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.23 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2BYE, Strand ID: A Detail

Release date

2005-11-06

Citation

Backbone 1H, 13C, and 15N resonance assignments for the two 13 kD Ras associating domains (RA1 and RA2) from phospholipase C epsilon
Harris, R., Bunney, T.D., Katan, M., Driscoll, P.C.
J. Biomol. NMR (2005), 33, 138-138, PubMed 16258837 , DOI 10.1007/s10858-005-3094-0 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 6635 released on 2005-11-06
Title Backbone 1H, 13C, and 15N Resonance Assignments for the 13kD Ras Association Domain 2 (RA2) from Phospholipase C (epsilon)

Related entities 1. RA1, : 1 : 2 : 17 entities Detail

Interaction partners 1. RA1, : 5 interactors Detail

More details for 5 interactors identified by 3 citations

Experiments performed 11 experiments Detail

1 1H15N HSQC

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian UnityPlus (500 MHz)

Sample #1

Temperature 298 K, pH 7.0 (±0.2), Details 15N labelled RA1

#	Name	Isotope labeling	Type	Concentration
1	ra1	[U-15N]	protein	1 mM
2	sodium chloride		salt	250 mM
3	DTT		buffer	5 mM
4	TRIS		buffer	25 mM
5	EDTA		buffer	0.1 mM

Sample #2

Temperature 298 K, pH 7.0 (±0.2), Details 13C/15N labelled RA1

#	Name	Isotope labeling	Type	Concentration
6	ra1	[U-13C; U-15N]	protein	1 mM
7	sodium chloride		salt	250 mM
8	DTT		buffer	5 mM
9	TRIS		buffer	25 mM
10	EDTA		buffer	0.1 mM

2 13C1H HSQC

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian UnityPlus (500 MHz)

Sample #1

Temperature 298 K, pH 7.0 (±0.2), Details 15N labelled RA1

#	Name	Isotope labeling	Type	Concentration
1	ra1	[U-15N]	protein	1 mM
2	sodium chloride		salt	250 mM
3	DTT		buffer	5 mM
4	TRIS		buffer	25 mM
5	EDTA		buffer	0.1 mM

Sample #2

Temperature 298 K, pH 7.0 (±0.2), Details 13C/15N labelled RA1

#	Name	Isotope labeling	Type	Concentration
6	ra1	[U-13C; U-15N]	protein	1 mM
7	sodium chloride		salt	250 mM
8	DTT		buffer	5 mM
9	TRIS		buffer	25 mM
10	EDTA		buffer	0.1 mM

3 HNCA

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian UnityPlus (500 MHz)

Sample #1

Temperature 298 K, pH 7.0 (±0.2), Details 15N labelled RA1

#	Name	Isotope labeling	Type	Concentration
1	ra1	[U-15N]	protein	1 mM
2	sodium chloride		salt	250 mM
3	DTT		buffer	5 mM
4	TRIS		buffer	25 mM
5	EDTA		buffer	0.1 mM

Sample #2

Temperature 298 K, pH 7.0 (±0.2), Details 13C/15N labelled RA1

#	Name	Isotope labeling	Type	Concentration
6	ra1	[U-13C; U-15N]	protein	1 mM
7	sodium chloride		salt	250 mM
8	DTT		buffer	5 mM
9	TRIS		buffer	25 mM
10	EDTA		buffer	0.1 mM

4 HNcoCA

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian UnityPlus (500 MHz)

Sample #1

Temperature 298 K, pH 7.0 (±0.2), Details 15N labelled RA1

#	Name	Isotope labeling	Type	Concentration
1	ra1	[U-15N]	protein	1 mM
2	sodium chloride		salt	250 mM
3	DTT		buffer	5 mM
4	TRIS		buffer	25 mM
5	EDTA		buffer	0.1 mM

Sample #2

Temperature 298 K, pH 7.0 (±0.2), Details 13C/15N labelled RA1

#	Name	Isotope labeling	Type	Concentration
6	ra1	[U-13C; U-15N]	protein	1 mM
7	sodium chloride		salt	250 mM
8	DTT		buffer	5 mM
9	TRIS		buffer	25 mM
10	EDTA		buffer	0.1 mM

5 HNCACB

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian UnityPlus (500 MHz)

Sample #1

Temperature 298 K, pH 7.0 (±0.2), Details 15N labelled RA1

#	Name	Isotope labeling	Type	Concentration
1	ra1	[U-15N]	protein	1 mM
2	sodium chloride		salt	250 mM
3	DTT		buffer	5 mM
4	TRIS		buffer	25 mM
5	EDTA		buffer	0.1 mM

Sample #2

Temperature 298 K, pH 7.0 (±0.2), Details 13C/15N labelled RA1

#	Name	Isotope labeling	Type	Concentration
6	ra1	[U-13C; U-15N]	protein	1 mM
7	sodium chloride		salt	250 mM
8	DTT		buffer	5 mM
9	TRIS		buffer	25 mM
10	EDTA		buffer	0.1 mM

6 CBCAcoNH

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian UnityPlus (500 MHz)

Sample #1

Temperature 298 K, pH 7.0 (±0.2), Details 15N labelled RA1

#	Name	Isotope labeling	Type	Concentration
1	ra1	[U-15N]	protein	1 mM
2	sodium chloride		salt	250 mM
3	DTT		buffer	5 mM
4	TRIS		buffer	25 mM
5	EDTA		buffer	0.1 mM

Sample #2

Temperature 298 K, pH 7.0 (±0.2), Details 13C/15N labelled RA1

#	Name	Isotope labeling	Type	Concentration
6	ra1	[U-13C; U-15N]	protein	1 mM
7	sodium chloride		salt	250 mM
8	DTT		buffer	5 mM
9	TRIS		buffer	25 mM
10	EDTA		buffer	0.1 mM

7 HNCO

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian UnityPlus (500 MHz)

Sample #1

Temperature 298 K, pH 7.0 (±0.2), Details 15N labelled RA1

#	Name	Isotope labeling	Type	Concentration
1	ra1	[U-15N]	protein	1 mM
2	sodium chloride		salt	250 mM
3	DTT		buffer	5 mM
4	TRIS		buffer	25 mM
5	EDTA		buffer	0.1 mM

Sample #2

Temperature 298 K, pH 7.0 (±0.2), Details 13C/15N labelled RA1

#	Name	Isotope labeling	Type	Concentration
6	ra1	[U-13C; U-15N]	protein	1 mM
7	sodium chloride		salt	250 mM
8	DTT		buffer	5 mM
9	TRIS		buffer	25 mM
10	EDTA		buffer	0.1 mM

8 HAcacoNH

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian UnityPlus (500 MHz)

Sample #1

Temperature 298 K, pH 7.0 (±0.2), Details 15N labelled RA1

#	Name	Isotope labeling	Type	Concentration
1	ra1	[U-15N]	protein	1 mM
2	sodium chloride		salt	250 mM
3	DTT		buffer	5 mM
4	TRIS		buffer	25 mM
5	EDTA		buffer	0.1 mM

Sample #2

Temperature 298 K, pH 7.0 (±0.2), Details 13C/15N labelled RA1

#	Name	Isotope labeling	Type	Concentration
6	ra1	[U-13C; U-15N]	protein	1 mM
7	sodium chloride		salt	250 mM
8	DTT		buffer	5 mM
9	TRIS		buffer	25 mM
10	EDTA		buffer	0.1 mM

9 HCCH-TOCSY

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian UnityPlus (500 MHz)

Sample #1

Temperature 298 K, pH 7.0 (±0.2), Details 15N labelled RA1

#	Name	Isotope labeling	Type	Concentration
1	ra1	[U-15N]	protein	1 mM
2	sodium chloride		salt	250 mM
3	DTT		buffer	5 mM
4	TRIS		buffer	25 mM
5	EDTA		buffer	0.1 mM

Sample #2

Temperature 298 K, pH 7.0 (±0.2), Details 13C/15N labelled RA1

#	Name	Isotope labeling	Type	Concentration
6	ra1	[U-13C; U-15N]	protein	1 mM
7	sodium chloride		salt	250 mM
8	DTT		buffer	5 mM
9	TRIS		buffer	25 mM
10	EDTA		buffer	0.1 mM

10 13C NOESY

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian UnityPlus (500 MHz)

Sample #1

Temperature 298 K, pH 7.0 (±0.2), Details 15N labelled RA1

#	Name	Isotope labeling	Type	Concentration
1	ra1	[U-15N]	protein	1 mM
2	sodium chloride		salt	250 mM
3	DTT		buffer	5 mM
4	TRIS		buffer	25 mM
5	EDTA		buffer	0.1 mM

Sample #2

Temperature 298 K, pH 7.0 (±0.2), Details 13C/15N labelled RA1

#	Name	Isotope labeling	Type	Concentration
6	ra1	[U-13C; U-15N]	protein	1 mM
7	sodium chloride		salt	250 mM
8	DTT		buffer	5 mM
9	TRIS		buffer	25 mM
10	EDTA		buffer	0.1 mM

11 15N NOESY

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian UnityPlus (500 MHz)

Sample #1

Temperature 298 K, pH 7.0 (±0.2), Details 15N labelled RA1

#	Name	Isotope labeling	Type	Concentration
1	ra1	[U-15N]	protein	1 mM
2	sodium chloride		salt	250 mM
3	DTT		buffer	5 mM
4	TRIS		buffer	25 mM
5	EDTA		buffer	0.1 mM

Sample #2

Temperature 298 K, pH 7.0 (±0.2), Details 13C/15N labelled RA1

#	Name	Isotope labeling	Type	Concentration
6	ra1	[U-13C; U-15N]	protein	1 mM
7	sodium chloride		salt	250 mM
8	DTT		buffer	5 mM
9	TRIS		buffer	25 mM
10	EDTA		buffer	0.1 mM

null

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Found 1 Document (1.826 seconds)

BMRB: 6624

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. RA1, : 1 : 2 : 17 entities Detail

Interaction partners 1. RA1, : 5 interactors Detail

Experiments performed 11 experiments Detail

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