Solution structure of human prolactin
LPICPGGAAR CQVTLRDLFD RAVVLSHYIH NLSSEMFSEF DKRYTHGRGF ITKAINSCHT SSLATPEDKE QAQQMNQKDF LSLIVSILRS WNEPLYHLVT EVRGMQEAPE AILSKAVEIE EQTKRLLEGM ELIVSQVHPE TKENEIYPVW SGLPSLQMAD EESRLSAYYN LLHCLRRDSH KIDNYLKLLK CRIIHNNNC
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 78.3 % (1850 of 2362) | 76.6 % (945 of 1233) | 81.7 % (749 of 917) | 73.6 % (156 of 212) |
Backbone | 84.0 % (989 of 1178) | 84.6 % (336 of 397) | 84.9 % (501 of 590) | 79.6 % (152 of 191) |
Sidechain | 75.6 % (1040 of 1376) | 72.8 % (609 of 836) | 82.3 % (427 of 519) | 19.0 % (4 of 21) |
Aromatic | 48.8 % (81 of 166) | 53.0 % (44 of 83) | 45.7 % (37 of 81) | 0.0 % (0 of 2) |
Methyl | 91.2 % (206 of 226) | 91.2 % (103 of 113) | 91.2 % (103 of 113) |
1. human prolactin
LPICPGGAAR CQVTLRDLFD RAVVLSHYIH NLSSEMFSEF DKRYTHGRGF ITKAINSCHT SSLATPEDKE QAQQMNQKDF LSLIVSILRS WNEPLYHLVT EVRGMQEAPE AILSKAVEIE EQTKRLLEGM ELIVSQVHPE TKENEIYPVW SGLPSLQMAD EESRLSAYYN LLHCLRRDSH KIDNYLKLLK CRIIHNNNCTemperature 310 (±0.1) K, pH 8 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | human prolactin | [U-13C; U-15N] | 1 mM |
Atom type | Mol. common name | Atom group | Value | Ref. method | Ref. type | Shift ratio |
---|---|---|---|---|---|---|
13C | DSS | methyl protons | 0.0 ppm | external | indirect | 0.2514495 |
1H | DSS | methyl protons | 0.0 ppm | external | direct | 1.0 |
15N | DSS | methyl protons | 0.0 ppm | external | indirect | 0.1013291 |
Atom type | Mol. common name | Atom group | Value | Ref. method | Ref. type | Shift ratio |
---|---|---|---|---|---|---|
13C | DSS | methyl protons | 0.0 ppm | external | indirect | 0.2514495 |
1H | DSS | methyl protons | 0.0 ppm | external | direct | 1.0 |
15N | DSS | methyl protons | 0.0 ppm | external | indirect | 0.1013291 |
Atom type | Mol. common name | Atom group | Value | Ref. method | Ref. type | Shift ratio |
---|---|---|---|---|---|---|
13C | DSS | methyl protons | 0.0 ppm | external | indirect | 0.2514495 |
1H | DSS | methyl protons | 0.0 ppm | external | direct | 1.0 |
15N | DSS | methyl protons | 0.0 ppm | external | indirect | 0.1013291 |
Atom type | Mol. common name | Atom group | Value | Ref. method | Ref. type | Shift ratio |
---|---|---|---|---|---|---|
13C | DSS | methyl protons | 0.0 ppm | external | indirect | 0.2514495 |
1H | DSS | methyl protons | 0.0 ppm | external | direct | 1.0 |
15N | DSS | methyl protons | 0.0 ppm | external | indirect | 0.1013291 |
Varian Inova - 800 MHz
State isotropic, Temperature 310 (±0.1) K, pH 8 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | human prolactin | [U-13C; U-15N] | 1 mM |
Varian Inova - 800 MHz
State isotropic, Temperature 310 (±0.1) K, pH 8 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | human prolactin | [U-13C; U-15N] | 1 mM |
Varian Inova - 800 MHz
State isotropic, Temperature 310 (±0.1) K, pH 8 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | human prolactin | [U-13C; U-15N] | 1 mM |
Varian Inova - 800 MHz
State isotropic, Temperature 310 (±0.1) K, pH 8 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | human prolactin | [U-13C; U-15N] | 1 mM |
Varian Inova - 800 MHz
State isotropic, Temperature 310 (±0.1) K, pH 8 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | human prolactin | [U-13C; U-15N] | 1 mM |
Varian Inova - 800 MHz
State isotropic, Temperature 310 (±0.1) K, pH 8 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | human prolactin | [U-13C; U-15N] | 1 mM |
Varian Inova - 800 MHz
State isotropic, Temperature 310 (±0.1) K, pH 8 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | human prolactin | [U-13C; U-15N] | 1 mM |
Varian Inova - 800 MHz
State isotropic, Temperature 310 (±0.1) K, pH 8 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | human prolactin | [U-13C; U-15N] | 1 mM |
Varian Inova - 800 MHz
State isotropic, Temperature 310 (±0.1) K, pH 8 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | human prolactin | [U-13C; U-15N] | 1 mM |
Varian Inova - 800 MHz
State isotropic, Temperature 310 (±0.1) K, pH 8 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | human prolactin | [U-13C; U-15N] | 1 mM |
Properties
Disulfide bonds
NoneOther bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)
NoneNon-standard residues
NoneContent subtype: bmr6643_3.str
Assigned chemical shifts
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100 LPICPGGAARCQVTLRDLFDRAVVLSHYIHNLSSEMFSEFDKRYTHGRGFITKAINSCHTSSLATPEDKEQAQQMNQKDFLSLIVSILRSWNEPLYHLVT |||||||||||||| ||||||||||||||||||||||||||||||| | || ||||| ||||||| ||||||||| ||||||||||||||||||||||| LPICPGGAARCQVT.RDLFDRAVVLSHYIHNLSSEMFSEFDKRYTH.R.FI.KAINS..TSSLATP.DKEQAQQMN.KDFLSLIVSILRSWNEPLYHLVT -------110-------120-------130-------140-------150-------160-------170-------180-------190--------- EVRGMQEAPEAILSKAVEIEEQTKRLLEGMELIVSQVHPETKENEIYPVWSGLPSLQMADEESRLSAYYNLLHCLRRDSHKIDNYLKLLKCRIIHNNNC ||||| ||| ||||||||||||||||||||||||||||| ||||||||||| | | ||||||||||||||||||||||||||||||||||||| |||| EVRGM.EAP.AILSKAVEIEEQTKRLLEGMELIVSQVHP.TKENEIYPVWS.L..L.MADEESRLSAYYNLLHCLRRDSHKIDNYLKLLKCRII.NNNC
Comp_index_ID | Comp_ID | Atom_ID | CS value (ppm) |
---|---|---|---|
73 | GLN | CD | 180.35 |
74 | GLN | CD | 180.35 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 1233 | 874 | 70.9 |
13C chemical shifts | 917 | 717 | 78.2 |
15N chemical shifts | 224 | 140 | 62.5 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 397 | 317 | 79.8 |
13C chemical shifts | 398 | 312 | 78.4 |
15N chemical shifts | 191 | 138 | 72.3 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 836 | 557 | 66.6 |
13C chemical shifts | 519 | 405 | 78.0 |
15N chemical shifts | 33 | 2 | 6.1 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 118 | 104 | 88.1 |
13C chemical shifts | 118 | 103 | 87.3 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 83 | 38 | 45.8 |
13C chemical shifts | 81 | 32 | 39.5 |
15N chemical shifts | 2 | 0 | 0.0 |