BMRBj - BMREntryMaster

Found 1 Document (2.073 seconds)

BMRB: 6643

1RW5

Solution structure of human prolactin

Authors

Teilum, K., Hoch, J.C., Goffin, V., Kinet, S., Martial, J.A., Kragelund, B.B.

Assembly

human prolactin

Entity

1. human prolactin (polymer, Thiol state: all disulfide bound), 199 monomers, 22897.91 Da Detail

LPICPGGAAR CQVTLRDLFD RAVVLSHYIH NLSSEMFSEF DKRYTHGRGF ITKAINSCHT SSLATPEDKE QAQQMNQKDF LSLIVSILRS WNEPLYHLVT EVRGMQEAPE AILSKAVEIE EQTKRLLEGM ELIVSQVHPE TKENEIYPVW SGLPSLQMAD EESRLSAYYN LLHCLRRDSH KIDNYLKLLK CRIIHNNNC

Formula weight

22897.91 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.0 %, Completeness: 78.3 %, Completeness (bb): 84.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	78.3 % (1850 of 2362)	76.6 % (945 of 1233)	81.7 % (749 of 917)	73.6 % (156 of 212)
Backbone	84.0 % (989 of 1178)	84.6 % (336 of 397)	84.9 % (501 of 590)	79.6 % (152 of 191)
Sidechain	75.6 % (1040 of 1376)	72.8 % (609 of 836)	82.3 % (427 of 519)	19.0 % (4 of 21)
Aromatic	48.8 % (81 of 166)	53.0 % (44 of 83)	45.7 % (37 of 81)	0.0 % (0 of 2)
Methyl	91.2 % (206 of 226)	91.2 % (103 of 113)	91.2 % (103 of 113)

1. human prolactin

Show sample condition

Sample

Temperature 310 (±0.1) K, pH 8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	human prolactin	[U-13C; U-15N]		1 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.09 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.09 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.32 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1RW5, Strand ID: A Detail

Release date

2005-08-15

Citation

Solution structure of human prolactin
Teilum, K., Hoch, J.C., Goffin, V., Kinet, S., Martial, J.A., Kragelund, B.B.
J. Mol. Biol. (2005), 351, 810-823, PubMed 16045928 , DOI 10.1016/j.jmb.2005.06.042 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: P01236 released on 1986-07-21
Title PRL_HUMAN Entity Prolactin

Related entities 1. human prolactin, : 1 : 3 : 9 : 172 entities Detail

Interaction partners 1. human prolactin, : 2 interactors Detail

More details for 2 interactors identified by 2 citations

Experiments performed 10 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr6643_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr6643_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	chem_shift_list_1	Warning	1941		92.0 (chain: 1, length: 199)
1	Chemical shift references	chemical_shift_referencing	OK	3		No information

Assigned chemical shifts

Saveframe: chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 199, Sequence coverage: 92.0%
- Total number of assignments
  - ¹H chemical shifts: 1082
  - ¹³C chemical shifts: 719
  - ¹⁵N chemical shifts: 140
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
73	GLN	CD	180.35
74	GLN	CD	180.35

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	1233	874	70.9
¹³C chemical shifts	917	717	78.2
¹⁵N chemical shifts	224	140	62.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	397	317	79.8
¹³C chemical shifts	398	312	78.4
¹⁵N chemical shifts	191	138	72.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	836	557	66.6
¹³C chemical shifts	519	405	78.0
¹⁵N chemical shifts	33	2	6.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	118	104	88.1
¹³C chemical shifts	118	103	87.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	83	38	45.8
¹³C chemical shifts	81	32	39.5
¹⁵N chemical shifts	2	0	0.0

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Found 1 Document (2.073 seconds)

BMRB: 6643

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. human prolactin, : 1 : 3 : 9 : 172 entities Detail

Interaction partners 1. human prolactin, : 2 interactors Detail

Experiments performed 10 experiments Detail

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Chemical shift validation 3 contents Detail