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G83N TPR domain of Ppp5
Authors
Cliff, M.J., Harris, R., Barford, D., Ladbury, J.E., Williams, M.A.
Assembly
PP5-TPR-peptide complex
Entity
1. tetratricopeptide domain from Ppp5 (polymer, Thiol state: all free), 140 monomers, 16179.12 Da Detail

MSGHHHHHHT DPPADGALKR AEELKTQAND YFKAKDYENA IKFYSQAIEL NPSNAIYYGN RSLAYLRTEC YGYALNDATR AIELDKKYIK GYYRRAASNM ALGKFRAALR DYETVVKVKP HDKDAKMKYQ ECNKIVKQKA


2. tetratricopeptide domain from Ppp5 (polymer, Thiol state: not present), 6 monomers, 647.6946 Da Detail

XMEEVD


Total weight
16826.814 Da
Max. entity weight
16179.12 Da
Source organism
Homo sapiens
Exptl. method
NMR
Refine. method
ARIA
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 93.2 %, Completeness: 87.4 %, Completeness (bb): 87.6 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All87.4 % (1506 of 1724)88.5 % (800 of 904)85.5 % (570 of 667)88.9 % (136 of 153)
Backbone87.6 % (755 of 862)90.1 % (263 of 292)85.3 % (366 of 429)89.4 % (126 of 141)
Sidechain87.3 % (874 of 1001)87.7 % (537 of 612)86.7 % (327 of 377)83.3 % (10 of 12)
Aromatic82.1 % (133 of 162)82.7 % (67 of 81)81.5 % (66 of 81)
Methyl98.4 % (126 of 128)100.0 % (64 of 64)96.9 % (62 of 64)

1. tetratricopeptide domain from Ppp5

MSGHHHHHHT DPPADGALKR AEELKTQAND YFKAKDYENA IKFYSQAIEL NPSNAIYYGN RSLAYLRTEC YGYALNDATR AIELDKKYIK GYYRRAASNM ALGKFRAALR DYETVVKVKP HDKDAKMKYQ ECNKIVKQKA

2. tetratricopeptide domain from Ppp5

XMEEVD

Sample

Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1), Details pH 6.0 reducing conditions


#NameIsotope labelingTypeConcentration
1tetratricopeptide domain from Ppp5[U-13C; U-15N]protein0.75 (±0.4) mM
2Hsp90 pentapeptideligand0.75 (±0.4) mM
3MESbuffer50 (±0.2) mM
4DTTreducing agent5 (±0.1) mM

Protein Blocks Logo
Calculated from 19 models in PDB: 2BUG, Strand ID: A, B Detail


Release date
2006-04-04
Citation
Conformational diversity in the TPR domain-mediated interaction of protein phosphatase 5 with Hsp90
Cliff, M.J., Harris, R., Barford, D., Ladbury, J.E., Williams, M.A.
Structure (2006), 14, 415-426, PubMed 16531226 , DOI 10.1016/j.str.2005.12.009 ,
Related entities 1. tetratricopeptide domain from Ppp5, : 76 entities Detail
Experiments performed 10 experiments Detail
nullKeywords NMR assigment