BMRBj - BMREntryMaster

Found 1 Document (3.488 seconds)

BMRB: 6700

1TBA

Resonance Assignments of 30 kDa complexes of TFIID subunit TAF1 with TATA-binding protein

Authors

Mal, T.K., Liu, D., Masutomi, Y., Zheng, L., Nakatani, Y., Kokubo, T., Ikura, M.

Assembly

TBP associated factor 1 (TAF1)

Entity

1. dTAF1 (polymer, Thiol state: not present), 67 monomers, 7007.630 Da Detail

EGSIGNGLDL TGILFGNIDS EGRLLQDDDG EGRGGTGFDA ELRENIGSLS KLGLDSMLLE VIDLKEA

2. TBPc (polymer, Thiol state: all free), 192 monomers, 21279.69 Da Detail

AAPESEKDTS ATSGIVPTLQ NIVATVTLGC RLDLKTVALH ARNAEYNPKR FAAVIMRIRE PKTTALIFAS GKMVVTGAKS EDDSKLASRK YARIIQKIGF AAKFTDFKIQ NIVGSCDVKF PIRLEGLAFS HGTFSSYEPE LFPGLIYRMV KPKIVLLIFV SGKIVLTGAK QREEIYQAFE AIYPVLSEFR KM

Total weight

28287.32 Da

Max. entity weight

21279.69 Da

Source organism

Drosophila melanogaster , Saccharomyces cerevisiae

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.2 %, Completeness: 50.0 %, Completeness (bb): 73.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	50.0 % (1497 of 2995)	38.7 % (601 of 1552)	54.7 % (645 of 1180)	95.4 % (251 of 263)
Backbone	73.6 % (1130 of 1536)	64.0 % (341 of 533)	72.0 % (542 of 753)	98.8 % (247 of 250)
Sidechain	34.8 % (590 of 1694)	25.5 % (260 of 1019)	49.4 % (327 of 662)	23.1 % (3 of 13)
Aromatic	13.3 % (26 of 196)	18.4 % (18 of 98)	8.2 % (8 of 98)
Methyl	37.8 % (124 of 328)	32.9 % (54 of 164)	42.7 % (70 of 164)

1. dTAF1

EGSIGNGLDL TGILFGNIDS EGRLLQDDDG EGRGGTGFDA ELRENIGSLS KLGLDSMLLE VIDLKEA

2. TBPc

Show sample condition

Sample

Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	dTAF1	[U-15N; U-13C; U-2H]	0.8 ~ 1.0 mM
2	TBPc	[U-15N; U-13C; U-2H]	0.8 ~ 1.0 mM
3	phosphate		20 mM
4	Glycerol		5 % v/v
5	Na2SO4		250 mM
6	MgCl2		5 mM
7	DTT		10 mM
8	NaN3		0.05 mM

Release date

2005-10-27

Citation

Resonance assignments of 30 kDa complexes of TFIID subunit TAF1 with TATA-binding protein
Mal, T.K., Liu, D., Masutomi, Y., Zheng, L., Nakatani, Y., Kokubo, T., Ikura, M.
J. Biomol. NMR (2005), 33, 76-76, PubMed 16222567 , DOI 10.1007/s10858-005-1929-3 , Abstract

Related entities 1. dTAF1, : 1 : 2 : 2 entities Detail

Related entities 2. TBPc, : 9 : 136 entities Detail

Interaction partners 1. dTAF1, : 17 : 1 interactors Detail

More details for 18 interactors identified by 6 citations

Interaction partners 2. TBPc, : 73 : 1 interactors Detail

More details for 74 interactors identified by 21 citations

Experiments performed 4 experiments Detail

Chemical shift validation 4 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr6700_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr6700_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	Chemical_shift_assignment_of_dTAF1(11-77)	Warning	1451		98.5 (chain: 1, length: 67), 98.4 (chain: 2, length: 192)
1	Chemical shift references	chemical_shift_referencing	OK	3		No information

Assigned chemical shifts

Saveframe: Chemical_shift_assignment_of_dTAF1(11-77)
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 67, Sequence coverage: 98.5%
  - Entity_assembly_ID: 2, Chain length: 192, Sequence coverage: 98.4%
- Total number of assignments
  - ¹H chemical shifts: 601
  - ¹³C chemical shifts: 600
  - ¹⁵N chemical shifts: 250
- Completeness of assignments
  - Entity_assemble_ID: 1
  - Entity_assemble_ID: 2
- Redox state of CYS
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1
  - Chain_ID: 2

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
23	ARG	HH11	7.73
23	ARG	HH12	7.77
23	ARG	NH1	86.37

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	369	336	91.1
¹³C chemical shifts	275	237	86.2
¹⁵N chemical shifts	74	68	91.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	147	142	96.6
¹³C chemical shifts	134	118	88.1
¹⁵N chemical shifts	67	66	98.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	222	194	87.4
¹³C chemical shifts	141	119	84.4
¹⁵N chemical shifts	7	2	28.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	41	41	100.0
¹³C chemical shifts	41	41	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	10	10	100.0
¹³C chemical shifts	10	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	1183	181	15.3
¹³C chemical shifts	905	363	40.1
¹⁵N chemical shifts	203	181	89.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	386	181	46.9
¹³C chemical shifts	384	185	48.2
¹⁵N chemical shifts	183	181	98.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	797	0	0.0
¹³C chemical shifts	521	178	34.2
¹⁵N chemical shifts	20	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	128	0	0.0
¹³C chemical shifts	128	18	14.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	88	0	0.0
¹³C chemical shifts	88	0	0.0

Keywords NMR, TBP, dATF1, dTAF1, NMR structure

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Found 1 Document (3.488 seconds)

BMRB: 6700

Sample

Related entities 1. dTAF1, : 1 : 2 : 2 entities Detail

Related entities 2. TBPc, : 9 : 136 entities Detail

Interaction partners 1. dTAF1, : 17 : 1 interactors Detail

Interaction partners 2. TBPc, : 73 : 1 interactors Detail

Experiments performed 4 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 4 contents Detail