1H, 15N, 13C resonance assignment of the N-terminal domain of PilB from Neisseria meningitidis
MVPHTLSTLK TADNRPASVY LKKDKPTLIK FWASWCPLCL SELGQTEKWA QDAKFSSANL ITVASPGFLH EKKDGDFQKW YAGLNYPKLP VVTDNGGTIA QSLNISVYPS WALIGKDGDV QRIVKGSINE AQALALIRDP NADL
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 66.5 % (1120 of 1684) | 59.3 % (517 of 872) | 72.1 % (475 of 659) | 83.7 % (128 of 153) |
Backbone | 93.5 % (791 of 846) | 88.9 % (256 of 288) | 96.5 % (408 of 423) | 94.1 % (127 of 135) |
Sidechain | 47.2 % (459 of 973) | 44.7 % (261 of 584) | 53.1 % (197 of 371) | 5.6 % (1 of 18) |
Aromatic | 8.6 % (12 of 140) | 8.6 % (6 of 70) | 7.7 % (5 of 65) | 20.0 % (1 of 5) |
Methyl | 68.6 % (118 of 172) | 80.2 % (69 of 86) | 57.0 % (49 of 86) |
1. Dsbe
MVPHTLSTLK TADNRPASVY LKKDKPTLIK FWASWCPLCL SELGQTEKWA QDAKFSSANL ITVASPGFLH EKKDGDFQKW YAGLNYPKLP VVTDNGGTIA QSLNISVYPS WALIGKDGDV QRIVKGSINE AQALALIRDP NADLTemperature 298 (±0.1) K, pH 7 (±0.05)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Dsbe | [U-99% 13C; U-99% 15N] | 1 (±0.1) mM |