1H Chemical Shift Assignments for Diapause-Specific Peptide from the Leaf Beetle
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | disulfide | sing | 1:CYS7:SG | 1:CYS21:SG |
2 | disulfide | sing | 1:CYS11:SG | 1:CYS33:SG |
3 | disulfide | sing | 1:CYS22:SG | 1:CYS40:SG |
Polymer type: polypeptide(L)
Total | 1H | |
---|---|---|
All | 93.0 % (211 of 227) | 93.0 % (211 of 227) |
Backbone | 92.9 % (79 of 85) | 92.9 % (79 of 85) |
Sidechain | 93.0 % (132 of 142) | 93.0 % (132 of 142) |
Aromatic | 75.0 % (12 of 16) | 75.0 % (12 of 16) |
Methyl | 100.0 % (13 of 13) | 100.0 % (13 of 13) |
1. DSP
AVRIGPCDQV CPRIVPERHE CCRAHGRSGY AYCSGGGMYC NTemperature 298 (±0.1) K, pH 7.0 (±0.05)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | DSP | 0.15 mM | ||
2 | sodium phosphate | 10 mM | ||
3 | NaN3 | 0.2 mM | ||
4 | DSS | 0.05 mM | ||
5 | D2O | 5 % |