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BMRB: 6747

2AYJ

Assignment of 50S ribosomal protein L40e

Authors

Wu, B., Yee, A., Lukin, J., Semesi, A., Lemak, A., Ramelot, T., Kennedy, M., Arrowsmith, C.H.

Assembly

50S ribosomal protein L40e

Entity

1. 50S ribosomal protein L40e (polymer, Thiol state: all other bound), 56 monomers, 6444.820 Da Detail

MPLTDPAKLQ IVQQRVFLKK VCRKCGALNP IRATKCRRCH STNLRLKKKE LPTKKG

2. ZN (non-polymer), 65.409 Da

Total weight

6510.229 Da

Max. entity weight

6444.82 Da

Entity Connection

na 4 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS22:SG	2:ZN1:ZN
2	na	sing	1:CYS25:SG	2:ZN1:ZN
3	na	sing	1:CYS36:SG	2:ZN1:ZN
4	na	sing	1:CYS39:SG	2:ZN1:ZN

Source organism

Thermoplasma acidophilum

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.9 %, Completeness: 84.3 %, Completeness (bb): 88.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	84.3 % (592 of 702)	83.1 % (315 of 379)	86.8 % (231 of 266)	80.7 % (46 of 57)
Backbone	88.4 % (290 of 328)	87.3 % (96 of 110)	89.8 % (149 of 166)	86.5 % (45 of 52)
Sidechain	82.0 % (351 of 428)	81.4 % (219 of 269)	85.1 % (131 of 154)	20.0 % (1 of 5)
Aromatic	28.6 % (4 of 14)	42.9 % (3 of 7)	14.3 % (1 of 7)
Methyl	100.0 % (62 of 62)	100.0 % (31 of 31)	100.0 % (31 of 31)

1. 50S ribosomal protein L40e

MPLTDPAKLQ IVQQRVFLKK VCRKCGALNP IRATKCRRCH STNLRLKKKE LPTKKG

Show sample condition

Sample

Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	50S ribosomal protein L40e	[U-13C; U-15N]	1 mM
2	NaCl		450 mM
3	Phosphate		25 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.07 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.07 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.03 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.41 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2AYJ, Strand ID: A Detail

Release date

2006-02-22

Citation

Solution structure of ribosomal protein L40E, a unique C4 zinc finger protein encoded by archaeon Sulfolobus solfataricus
Wu, B., Yee, A., Lukin, J., Semesi, A., Lemak, A., Ramelot, T., Kennedy, M., Arrowsmith, C.H.
Protein Sci. (2008), 17, 589-596, PubMed 18218710 , DOI 10.1110/ps.073273008 , Abstract

Related entities 1. 50S ribosomal protein L40e, : 1 : 1 : 72 entities Detail

Experiments performed 15 experiments Detail

1 15N HSQC