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Found 1 Document (1.2 seconds)

BMRB: 6753

2ADZ

Backbone 1H,13C, and 15N chemical shift assignments for the PH1 domain of alpha-syntrophin

Authors

Yan, J., Wen, W., Xu, W., Long, J., Adams, M.E., Froehner, S.C., Zhang, M.

Assembly

split PH1 domain of alpha-syntrophin

Entity

1. split PH1 domain of alpha-syntrophin (polymer, Thiol state: all free), 178 monomers, 18616.55 Da Detail

ASGRRAPRTG LLELRCGAGS GAGGERWQRV LLSLAEDALT VSPADGEPGP EPEPAQLNGA AEPGAAPPQL PEALLLQREV SPYFKNSAGG TSVGWDSPPA SPLQRQPSSP GPQPRNLSEA KHVSLKMAYV SRRCTPTDPE PRYLEICAAD GQDAVFLRAK DEASARSWAG AIQAQIGT

Formula weight

18616.55 Da

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.3 %, Completeness: 81.8 %, Completeness (bb): 81.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	81.8 % (1574 of 1924)	88.7 % (895 of 1009)	69.7 % (517 of 742)	93.6 % (162 of 173)
Backbone	81.8 % (839 of 1026)	94.3 % (333 of 353)	68.8 % (355 of 516)	96.2 % (151 of 157)
Sidechain	82.6 % (874 of 1058)	84.6 % (555 of 656)	79.8 % (308 of 386)	68.8 % (11 of 16)
Aromatic	48.8 % (41 of 84)	81.0 % (34 of 42)	10.3 % (4 of 39)	100.0 % (3 of 3)
Methyl	91.3 % (157 of 172)	93.0 % (80 of 86)	89.5 % (77 of 86)

1. split PH domain

ASGRRAPRTG LLELRCGAGS GAGGERWQRV LLSLAEDALT VSPADGEPGP EPEPAQLNGA AEPGAAPPQL PEALLLQREV SPYFKNSAGG TSVGWDSPPA SPLQRQPSSP GPQPRNLSEA KHVSLKMAYV SRRCTPTDPE PRYLEICAAD GQDAVFLRAK DEASARSWAG AIQAQIGT

Show sample condition

Sample

Temperature 303 K, pH 7 (±0.5)

#	Name	Isotope labeling	Type	Concentration
1	split PH domain	[U-13C; U-15N]		1 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.77 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.77 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2ADZ, Strand ID: A Detail

Release date

2007-01-28

Citation

Structure of the split PH domain and distinct lipid-binding properties of the PH-PDZ supramodule of alpha-syntrophin
Yan, J., Wen, W., Xu, W., Long, J., Adams, M.E., Froehner, S.C., Zhang, M.
EMBO J. (2005), 24, 3985-3995, PubMed 16252003 , DOI 10.1038/sj.emboj.7600858 , Abstract

Related entities 1. split PH1 domain of alpha-syntrophin, : 1 : 12 entities Detail

Experiments performed 8 experiments Detail

1 HNCO

Instrument

Model Varian Inova (750 MHz)

Sample #1

Temperature 303 K, pH 7 (±0.5)

#	Name	Isotope labeling	Type	Concentration
1	split PH domain	[U-13C; U-15N]		1 mM

Sample #2

Temperature 303 K, pH 7 (±0.5)

#	Name	Isotope labeling	Type	Concentration
2	split PH domain	[U-15N]		1 mM

2 HNCA

Instrument

Model Varian Inova (750 MHz)

Sample #1

Temperature 303 K, pH 7 (±0.5)

#	Name	Isotope labeling	Type	Concentration
1	split PH domain	[U-13C; U-15N]		1 mM

Sample #2

Temperature 303 K, pH 7 (±0.5)

#	Name	Isotope labeling	Type	Concentration
2	split PH domain	[U-15N]		1 mM

3 HN(CO)CA

Instrument

Model Varian Inova (750 MHz)

Sample #1

Temperature 303 K, pH 7 (±0.5)

#	Name	Isotope labeling	Type	Concentration
1	split PH domain	[U-13C; U-15N]		1 mM

Sample #2

Temperature 303 K, pH 7 (±0.5)

#	Name	Isotope labeling	Type	Concentration
2	split PH domain	[U-15N]		1 mM

4 HNCACB

Instrument

Model Varian Inova (750 MHz)

Sample #1

Temperature 303 K, pH 7 (±0.5)

#	Name	Isotope labeling	Type	Concentration
1	split PH domain	[U-13C; U-15N]		1 mM

Sample #2

Temperature 303 K, pH 7 (±0.5)

#	Name	Isotope labeling	Type	Concentration
2	split PH domain	[U-15N]		1 mM

5 CBCA(CO)NH

Instrument

Model Varian Inova (750 MHz)

Sample #1

Temperature 303 K, pH 7 (±0.5)

#	Name	Isotope labeling	Type	Concentration
1	split PH domain	[U-13C; U-15N]		1 mM

Sample #2

Temperature 303 K, pH 7 (±0.5)

#	Name	Isotope labeling	Type	Concentration
2	split PH domain	[U-15N]		1 mM

6 HCCH-TOCSY

Instrument

Model Varian Inova (750 MHz)

Sample #1

Temperature 303 K, pH 7 (±0.5)

#	Name	Isotope labeling	Type	Concentration
1	split PH domain	[U-13C; U-15N]		1 mM

Sample #2

Temperature 303 K, pH 7 (±0.5)

#	Name	Isotope labeling	Type	Concentration
2	split PH domain	[U-15N]		1 mM

7 3D 15N-separated NOESY

Instrument

Model Varian Inova (750 MHz)

Sample #1

Temperature 303 K, pH 7 (±0.5)

#	Name	Isotope labeling	Type	Concentration
1	split PH domain	[U-13C; U-15N]		1 mM

Sample #2

Temperature 303 K, pH 7 (±0.5)

#	Name	Isotope labeling	Type	Concentration
2	split PH domain	[U-15N]		1 mM

8 3D 15C-separated NOESY

Instrument

Model Varian Inova (750 MHz)

Sample #1

Temperature 303 K, pH 7 (±0.5)

#	Name	Isotope labeling	Type	Concentration
1	split PH domain	[U-13C; U-15N]		1 mM

Sample #2

Temperature 303 K, pH 7 (±0.5)

#	Name	Isotope labeling	Type	Concentration
2	split PH domain	[U-15N]		1 mM

nullKeywords split PH domain

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Found 1 Document (1.2 seconds)

BMRB: 6753

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. split PH1 domain of alpha-syntrophin, : 1 : 12 entities Detail

Experiments performed 8 experiments Detail

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