BMRBj - BMREntryMaster

Found 1 Document (2.3 seconds)

BMRB: 6763

2HST

1H, 15N and 13C resonance assignments of the middle domain of human release factor eRF1

Authors

Polshakov, V.I., Birdsall, B., Ivanova, E., Kisselev, L.L.

Assembly

middle domain of human eRF1

Entity

1. middle domain of human eRF1 (polymer, Thiol state: not present), 136 monomers, 14946.83 Da Detail

LSDDSKFGFI VIDGSGALFG TLQGNTREVL HKFTVDLPKK HGRGGQSALR FARLRMEKRH NYVRKVAETA VQLFISGDKV NVAGLVLAGS ADFKTELSQS DMFDQRLQSK VLKLVDISYG GENGFNQAIE LSTEVL

Formula weight

14946.83 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.9 %, Completeness: 81.6 %, Completeness (bb): 93.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	81.6 % (1285 of 1575)	83.1 % (678 of 816)	76.8 % (470 of 612)	93.2 % (137 of 147)
Backbone	93.1 % (758 of 814)	92.3 % (263 of 285)	93.9 % (370 of 394)	92.6 % (125 of 135)
Sidechain	72.8 % (643 of 883)	78.2 % (415 of 531)	63.5 % (216 of 340)	100.0 % (12 of 12)
Aromatic	65.3 % (77 of 118)	81.4 % (48 of 59)	49.2 % (29 of 59)
Methyl	84.6 % (137 of 162)	97.5 % (79 of 81)	71.6 % (58 of 81)

1. middle domain of human release factor eRF1

LSDDSKFGFI VIDGSGALFG TLQGNTREVL HKFTVDLPKK HGRGGQSALR FARLRMEKRH NYVRKVAETA VQLFISGDKV NVAGLVLAGS ADFKTELSQS DMFDQRLQSK VLKLVDISYG GENGFNQAIE LSTEVL

Show sample condition

Sample

Pressure 1 (±0.01) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	middle domain of human release factor eRF1	[U-95% 13C; U-95% 15N]	protein	1.2 (±0.2) mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.09 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.09 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.03 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 25 models in PDB: 2HST, Strand ID: A Detail

Release date

2006-03-09

Citation

NMR assignments of the middle domain of human polypeptide release factor eRF1
Ivanova, E.V., Kolosov, P.M., Birdsall, B., Kisselev, L.L., Polshakov, V.I.
J. Biomol. NMR (2006), 36 Suppl 1, 8-8, PubMed 16502324 , DOI 10.1007/s10858-005-4741-1 , Abstract

Related entities 1. middle domain of human eRF1, : 1 : 16 : 4 : 91 entities Detail

Interaction partners 1. middle domain of human eRF1, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 11 experiments Detail

1 1H15N_HSQC

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Bruker Avance (600 MHz)

#3 Model Varian INOVA (800 MHz)

#4 Model Varian Unity Plus (500 MHz)

Sample #1

Pressure 1 (±0.01) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	middle domain of human release factor eRF1	[U-95% 13C; U-95% 15N]	protein	1.2 (±0.2) mM

Sample #2

Pressure 1 (±0.01) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	middle domain of human release factor eRF1	[U-95% 15N]	protein	1.2 (±0.2) mM

2 1H13C_HSQC

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Bruker Avance (600 MHz)

#3 Model Varian INOVA (800 MHz)

#4 Model Varian Unity Plus (500 MHz)

Sample #1

Pressure 1 (±0.01) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	middle domain of human release factor eRF1	[U-95% 13C; U-95% 15N]	protein	1.2 (±0.2) mM

Sample #2

Pressure 1 (±0.01) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	middle domain of human release factor eRF1	[U-95% 15N]	protein	1.2 (±0.2) mM

3 HNCO

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Bruker Avance (600 MHz)

#3 Model Varian INOVA (800 MHz)

#4 Model Varian Unity Plus (500 MHz)

Sample #1

Pressure 1 (±0.01) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	middle domain of human release factor eRF1	[U-95% 13C; U-95% 15N]	protein	1.2 (±0.2) mM

Sample #2

Pressure 1 (±0.01) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	middle domain of human release factor eRF1	[U-95% 15N]	protein	1.2 (±0.2) mM

4 HNCA

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Bruker Avance (600 MHz)

#3 Model Varian INOVA (800 MHz)

#4 Model Varian Unity Plus (500 MHz)

Sample #1

Pressure 1 (±0.01) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	middle domain of human release factor eRF1	[U-95% 13C; U-95% 15N]	protein	1.2 (±0.2) mM

Sample #2

Pressure 1 (±0.01) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	middle domain of human release factor eRF1	[U-95% 15N]	protein	1.2 (±0.2) mM

5 HN(CO)CA

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Bruker Avance (600 MHz)

#3 Model Varian INOVA (800 MHz)

#4 Model Varian Unity Plus (500 MHz)

Sample #1

Pressure 1 (±0.01) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	middle domain of human release factor eRF1	[U-95% 13C; U-95% 15N]	protein	1.2 (±0.2) mM

Sample #2

Pressure 1 (±0.01) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	middle domain of human release factor eRF1	[U-95% 15N]	protein	1.2 (±0.2) mM

6 HNCACB

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Bruker Avance (600 MHz)

#3 Model Varian INOVA (800 MHz)

#4 Model Varian Unity Plus (500 MHz)

Sample #1

Pressure 1 (±0.01) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	middle domain of human release factor eRF1	[U-95% 13C; U-95% 15N]	protein	1.2 (±0.2) mM

Sample #2

Pressure 1 (±0.01) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	middle domain of human release factor eRF1	[U-95% 15N]	protein	1.2 (±0.2) mM

7 CBCA(CO)NH

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Bruker Avance (600 MHz)

#3 Model Varian INOVA (800 MHz)

#4 Model Varian Unity Plus (500 MHz)

Sample #1

Pressure 1 (±0.01) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	middle domain of human release factor eRF1	[U-95% 13C; U-95% 15N]	protein	1.2 (±0.2) mM

Sample #2

Pressure 1 (±0.01) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	middle domain of human release factor eRF1	[U-95% 15N]	protein	1.2 (±0.2) mM

8 HBHA(CO)NH

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Bruker Avance (600 MHz)

#3 Model Varian INOVA (800 MHz)

#4 Model Varian Unity Plus (500 MHz)

Sample #1

Pressure 1 (±0.01) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	middle domain of human release factor eRF1	[U-95% 13C; U-95% 15N]	protein	1.2 (±0.2) mM

Sample #2

Pressure 1 (±0.01) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	middle domain of human release factor eRF1	[U-95% 15N]	protein	1.2 (±0.2) mM

9 HCCH_TOCSY

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Bruker Avance (600 MHz)

#3 Model Varian INOVA (800 MHz)

#4 Model Varian Unity Plus (500 MHz)

Sample #1

Pressure 1 (±0.01) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	middle domain of human release factor eRF1	[U-95% 13C; U-95% 15N]	protein	1.2 (±0.2) mM

Sample #2

Pressure 1 (±0.01) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	middle domain of human release factor eRF1	[U-95% 15N]	protein	1.2 (±0.2) mM

10 15N_NOESY_HSQC

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Bruker Avance (600 MHz)

#3 Model Varian INOVA (800 MHz)

#4 Model Varian Unity Plus (500 MHz)

Sample #1

Pressure 1 (±0.01) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	middle domain of human release factor eRF1	[U-95% 13C; U-95% 15N]	protein	1.2 (±0.2) mM

Sample #2

Pressure 1 (±0.01) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	middle domain of human release factor eRF1	[U-95% 15N]	protein	1.2 (±0.2) mM

11 13C_NOESY_HSQC

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Bruker Avance (600 MHz)

#3 Model Varian INOVA (800 MHz)

#4 Model Varian Unity Plus (500 MHz)

Sample #1

Pressure 1 (±0.01) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	middle domain of human release factor eRF1	[U-95% 13C; U-95% 15N]	protein	1.2 (±0.2) mM

Sample #2

Pressure 1 (±0.01) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	middle domain of human release factor eRF1	[U-95% 15N]	protein	1.2 (±0.2) mM

null

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Found 1 Document (2.3 seconds)

BMRB: 6763

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. middle domain of human eRF1, : 1 : 16 : 4 : 91 entities Detail

Interaction partners 1. middle domain of human eRF1, : 1 interactors Detail

Experiments performed 11 experiments Detail

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