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BMRB: 6769

2LKI

Complete assignments of a hypothetical protein NE2066, from Nitrosomonas_europaea. Northeast Structural Genomics Consortium Target NeT1.

Authors

Srisailam, S., Yee, A., Lukin, J.A., Semesi, A., Arrowsmith, C.H.

Assembly

NE2066

Entity

1. NE2066 (polymer, Thiol state: not present), 83 monomers, 9169.228 Da Detail

MQHLEAVRNI LGDVLNLGER KHTLTASSVL LGNIPELDSM AVVNVITALE EYFDFSVDDD EISAQTFETL GSLALFVEHK LSH

Formula weight

9169.228 Da

Source organism

Nitrosomonas europaea

Exptl. method

NMR

Refine. method

restrained molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 94.1 %, Completeness (bb): 97.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	94.1 % (884 of 939)	95.0 % (452 of 476)	93.1 % (349 of 375)	94.3 % (83 of 88)
Backbone	97.6 % (484 of 496)	98.2 % (166 of 169)	98.4 % (241 of 245)	93.9 % (77 of 82)
Sidechain	89.7 % (468 of 522)	91.5 % (281 of 307)	86.6 % (181 of 209)	100.0 % (6 of 6)
Aromatic	62.5 % (40 of 64)	65.6 % (21 of 32)	59.4 % (19 of 32)
Methyl	94.3 % (115 of 122)	96.7 % (59 of 61)	91.8 % (56 of 61)

1. NE2066

MQHLEAVRNI LGDVLNLGER KHTLTASSVL LGNIPELDSM AVVNVITALE EYFDFSVDDD EISAQTFETL GSLALFVEHK LSH

Show sample condition

Sample

Temperature 298 (±0.1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	protein	[U-15N; U-13C]	protein	1 (±0.2) mM
2	MOPS		buffer	10 (±0.1) mM
3	NaCl		salt	450 (±0.1) mM
4	DTT		reducing agent	10 (±0.1) mM
5	Zinc Sulfate		additive	10 (±0.1) uM
6	Benzamidine		additive	1 (±0.1) mM
7	Sodium azide		additive	0.01 (±0.1) %
8	D2O		solvent	5 (±1.0) %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.1 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.1 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.09 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.58 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LKI, Strand ID: A Detail

Release date

2007-02-05

Citation

Solution structure of acyl carrier protein from Nitrosomonas europaea
Srisailam, S., Lukin, J.A., Yee, A., Semesi, A., Arrowsmith, C.H.
Proteins (2006), 64, 800-803, PubMed 16741959 , DOI 10.1002/prot.21016 , Abstract

Related entities 1. NE2066, : 1 : 77 entities Detail

Experiments performed 15 experiments Detail

1 1H15N_HSQC