BMRBj - BMREntryMaster

Found 1 Document (2.502 seconds)

BMRB: 6787

2K3H

Backbone 1H, 13C, and 15N Chemical Shift Assignments for rabphilin C2A domain

Authors

Montaville, P., Kim, H., Vijayan, V., Becker, S., Zweckstetter, M.

Assembly

rabphilin C2A domain

Entity

1. C2A domain (polymer, Thiol state: all free), 140 monomers, 16048.97 Da Detail

EANSYDSDEA TTLGALEFSL LYDQDNSNLQ CTIIRAKGLK PMDSNGLADP YVKLHLLPGA SKSNKLRTKT LRNTRNPVWN ETLQYHGITE EDMQRKTLRI SVCDEDKFGH NEFIGETRFS LKKLKANQRK NFNICLERVI

2. CA (non-polymer), 40.078 × 3 Da

Total weight

16169.204 Da

Max. entity weight

16048.97 Da

Source organism

Mus musculus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 92.2 %, Completeness (bb): 98.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	92.2 % (1534 of 1664)	93.9 % (821 of 874)	88.1 % (560 of 636)	99.4 % (153 of 154)
Backbone	98.6 % (820 of 832)	98.2 % (278 of 283)	98.5 % (407 of 413)	99.3 % (135 of 136)
Sidechain	87.7 % (846 of 965)	91.9 % (543 of 591)	80.1 % (285 of 356)	100.0 % (18 of 18)
Aromatic	47.2 % (50 of 106)	84.9 % (45 of 53)	7.7 % (4 of 52)	100.0 % (1 of 1)
Methyl	92.5 % (135 of 146)	94.5 % (69 of 73)	90.4 % (66 of 73)

1. C2A domain

EANSYDSDEA TTLGALEFSL LYDQDNSNLQ CTIIRAKGLK PMDSNGLADP YVKLHLLPGA SKSNKLRTKT LRNTRNPVWN ETLQYHGITE EDMQRKTLRI SVCDEDKFGH NEFIGETRFS LKKLKANQRK NFNICLERVI

Show sample condition

Sample

Temperature 301 (±0.5) K, pH 5.0 (±0.01), Details Na acetate 20mM pH5.0 NaCl 150mM CaCl2 20 mM DTT 1mM

#	Name	Isotope labeling	Concentration
1	rabphilin C2A domain	[U-13C; U-15N]	1 (±0.1) mM
2	Na acetate		20 mM
3	NaCl		150 mM
4	CaCl2		20 mM
5	DTT		1 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 2.46 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 2.46 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.32 ppm, Outliers: 1 Detail

Release date

2005-08-22

Citation

1H, 15N, and 13C resonance assignment of the C2A domain of rabphilin 3A
Montaville, P., Kim, H., Vijayan, V., Becker, S., Zweckstetter, M.
J. Biomol. NMR (2006), 36 Suppl 1, 20-20, PubMed 16609836 , DOI 10.1007/s10858-005-6051-z , Abstract

Related entities 1. C2A domain, : 1 : 2 : 3 : 222 entities Detail

Interaction partners 1. C2A domain, : 10 interactors Detail

More details for 10 interactors identified by 6 citations

Experiments performed 13 experiments Detail

1 2D [15N, 1H] HSQC