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BMRB: 6834

2A7O

Solution Structure of the hSet2/HYPB SRI domain

Authors

Li, M., Phatnani, H.P., Greenleaf, A., Zhou, P.

Assembly

Huntingtin interacting protein B isoform 1

Entity

1. Huntingtin interacting protein B isoform 1 (polymer, Thiol state: all free), 112 monomers, 13119.95 Da Detail

GSHMTAEADT SSELAKKSKE VFRKEMSQFI VQCLNPYRKP DCKVGRITTT EDFKHLARKL THGVMNKELK YCKNPEDLEC NENVKHKTKE YIKKYMQKFG AVYKPKEDTE LE

Formula weight

13119.95 Da

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.1 %, Completeness: 84.9 %, Completeness (bb): 80.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	84.9 % (1165 of 1372)	91.5 % (667 of 729)	73.8 % (389 of 527)	94.0 % (109 of 116)
Backbone	80.6 % (535 of 664)	95.5 % (214 of 224)	65.7 % (218 of 332)	95.4 % (103 of 108)
Sidechain	90.1 % (735 of 816)	89.9 % (454 of 505)	90.8 % (275 of 303)	75.0 % (6 of 8)
Aromatic	83.3 % (80 of 96)	83.3 % (40 of 48)	83.3 % (40 of 48)
Methyl	100.0 % (90 of 90)	100.0 % (45 of 45)	100.0 % (45 of 45)

1. the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1

GSHMTAEADT SSELAKKSKE VFRKEMSQFI VQCLNPYRKP DCKVGRITTT EDFKHLARKL THGVMNKELK YCKNPEDLEC NENVKHKTKE YIKKYMQKFG AVYKPKEDTE LE

Show sample condition

Sample

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
1	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-15N]	2.0 mM
2	KCl		100 mM
3	H2O		90 %
4	D2O		10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.08 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.08 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 3 Detail

Release date

2005-12-22

Citation 1

NMR assignment of the SRI domain of human Set2/HYPB
Li, M., Phatnani, H.P., Greenleaf, A.L., Zhou, P.
J. Biomol. NMR (2006), 36 Suppl 1, 5-5, PubMed 16435090 , DOI 10.1007/s10858-005-4690-8 , Abstract

Show more 1 citaion

Citation 2

Solution structure of the Set2-Rpb1 interacting domain of human Set2 and its interaction with the hyperphosphorylated C-terminal domain of Rpb1
Li, M., Phatnani, H.P., Guan, Z., Sage, H., Greenleaf, A.L., Zhou, P.
Proc. Natl. Acad. Sci. U. S. A. (2005), 102, 17636-17641, PubMed 16314571 , DOI 10.1073/pnas.0506350102 , Abstract

Hide non-primary 1 citaion

Entries sharing articles Swiss-Prot: 2 entries Detail

  Swiss-Prot: Q9BYW2 released on 2006-10-17
    Title SETD2_HUMAN Entity Histone-lysine N-methyltransferase SETD2
  Swiss-Prot: P24928 released on 1992-03-01
    Title RPB1_HUMAN Entity DNA-directed RNA polymerase II subunit RPB1

Related entities 1. Huntingtin interacting protein B isoform 1, : 1 : 5 : 5 entities Detail

Interaction partners 1. Huntingtin interacting protein B isoform 1, : 14 interactors Detail

More details for 14 interactors identified by 10 citations

Experiments performed 14 experiments Detail

1 3D_15N-separated_NOESY

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
1	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-15N]	2.0 mM
2	KCl		100 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
5	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-95% 13C; U-98% 15N]	2.0 mM
6	KCl		100 mM
7	H2O		90 %
8	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
9	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-15N]-Lys	2.0 mM
10	KCl		100 mM
11	H2O		90 %
12	D2O		10 %

Sample #4

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
13	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-95% 13C; U-98% 15N]	2.0 mM
14	KCl		100 mM
15	D2O		100 %

Sample #5

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Concentration
16	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	2.0 mM
17	KCl	100 mM
18	D2O	100 %

Sample #6

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
19	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-10% 13C]	2.0 mM
20	KCl		100 mM
21	D2O		100 %

2 3D_13C-separated_NOESY

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
1	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-15N]	2.0 mM
2	KCl		100 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
5	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-95% 13C; U-98% 15N]	2.0 mM
6	KCl		100 mM
7	H2O		90 %
8	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
9	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-15N]-Lys	2.0 mM
10	KCl		100 mM
11	H2O		90 %
12	D2O		10 %

Sample #4

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
13	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-95% 13C; U-98% 15N]	2.0 mM
14	KCl		100 mM
15	D2O		100 %

Sample #5

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Concentration
16	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	2.0 mM
17	KCl	100 mM
18	D2O	100 %

Sample #6

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
19	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-10% 13C]	2.0 mM
20	KCl		100 mM
21	D2O		100 %

3 2D NOESY

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
1	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-15N]	2.0 mM
2	KCl		100 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
5	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-95% 13C; U-98% 15N]	2.0 mM
6	KCl		100 mM
7	H2O		90 %
8	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
9	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-15N]-Lys	2.0 mM
10	KCl		100 mM
11	H2O		90 %
12	D2O		10 %

Sample #4

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
13	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-95% 13C; U-98% 15N]	2.0 mM
14	KCl		100 mM
15	D2O		100 %

Sample #5

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Concentration
16	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	2.0 mM
17	KCl	100 mM
18	D2O	100 %

Sample #6

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
19	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-10% 13C]	2.0 mM
20	KCl		100 mM
21	D2O		100 %

4 2D TOCSY

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
1	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-15N]	2.0 mM
2	KCl		100 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
5	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-95% 13C; U-98% 15N]	2.0 mM
6	KCl		100 mM
7	H2O		90 %
8	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
9	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-15N]-Lys	2.0 mM
10	KCl		100 mM
11	H2O		90 %
12	D2O		10 %

Sample #4

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
13	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-95% 13C; U-98% 15N]	2.0 mM
14	KCl		100 mM
15	D2O		100 %

Sample #5

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Concentration
16	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	2.0 mM
17	KCl	100 mM
18	D2O	100 %

Sample #6

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
19	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-10% 13C]	2.0 mM
20	KCl		100 mM
21	D2O		100 %

5 HNCA

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
1	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-15N]	2.0 mM
2	KCl		100 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
5	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-95% 13C; U-98% 15N]	2.0 mM
6	KCl		100 mM
7	H2O		90 %
8	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
9	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-15N]-Lys	2.0 mM
10	KCl		100 mM
11	H2O		90 %
12	D2O		10 %

Sample #4

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
13	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-95% 13C; U-98% 15N]	2.0 mM
14	KCl		100 mM
15	D2O		100 %

Sample #5

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Concentration
16	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	2.0 mM
17	KCl	100 mM
18	D2O	100 %

Sample #6

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
19	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-10% 13C]	2.0 mM
20	KCl		100 mM
21	D2O		100 %

6 HN(CO)CA

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
1	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-15N]	2.0 mM
2	KCl		100 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
5	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-95% 13C; U-98% 15N]	2.0 mM
6	KCl		100 mM
7	H2O		90 %
8	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
9	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-15N]-Lys	2.0 mM
10	KCl		100 mM
11	H2O		90 %
12	D2O		10 %

Sample #4

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
13	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-95% 13C; U-98% 15N]	2.0 mM
14	KCl		100 mM
15	D2O		100 %

Sample #5

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Concentration
16	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	2.0 mM
17	KCl	100 mM
18	D2O	100 %

Sample #6

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
19	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-10% 13C]	2.0 mM
20	KCl		100 mM
21	D2O		100 %

7 HN(CA)CB

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
1	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-15N]	2.0 mM
2	KCl		100 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
5	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-95% 13C; U-98% 15N]	2.0 mM
6	KCl		100 mM
7	H2O		90 %
8	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
9	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-15N]-Lys	2.0 mM
10	KCl		100 mM
11	H2O		90 %
12	D2O		10 %

Sample #4

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
13	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-95% 13C; U-98% 15N]	2.0 mM
14	KCl		100 mM
15	D2O		100 %

Sample #5

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Concentration
16	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	2.0 mM
17	KCl	100 mM
18	D2O	100 %

Sample #6

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
19	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-10% 13C]	2.0 mM
20	KCl		100 mM
21	D2O		100 %

8 HN(COCA)CB

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
1	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-15N]	2.0 mM
2	KCl		100 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
5	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-95% 13C; U-98% 15N]	2.0 mM
6	KCl		100 mM
7	H2O		90 %
8	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
9	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-15N]-Lys	2.0 mM
10	KCl		100 mM
11	H2O		90 %
12	D2O		10 %

Sample #4

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
13	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-95% 13C; U-98% 15N]	2.0 mM
14	KCl		100 mM
15	D2O		100 %

Sample #5

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Concentration
16	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	2.0 mM
17	KCl	100 mM
18	D2O	100 %

Sample #6

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
19	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-10% 13C]	2.0 mM
20	KCl		100 mM
21	D2O		100 %

9 HNCO

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
1	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-15N]	2.0 mM
2	KCl		100 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
5	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-95% 13C; U-98% 15N]	2.0 mM
6	KCl		100 mM
7	H2O		90 %
8	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
9	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-15N]-Lys	2.0 mM
10	KCl		100 mM
11	H2O		90 %
12	D2O		10 %

Sample #4

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
13	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-95% 13C; U-98% 15N]	2.0 mM
14	KCl		100 mM
15	D2O		100 %

Sample #5

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Concentration
16	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	2.0 mM
17	KCl	100 mM
18	D2O	100 %

Sample #6

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
19	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-10% 13C]	2.0 mM
20	KCl		100 mM
21	D2O		100 %

10 HNCA-J

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
1	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-15N]	2.0 mM
2	KCl		100 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
5	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-95% 13C; U-98% 15N]	2.0 mM
6	KCl		100 mM
7	H2O		90 %
8	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
9	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-15N]-Lys	2.0 mM
10	KCl		100 mM
11	H2O		90 %
12	D2O		10 %

Sample #4

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
13	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-95% 13C; U-98% 15N]	2.0 mM
14	KCl		100 mM
15	D2O		100 %

Sample #5

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Concentration
16	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	2.0 mM
17	KCl	100 mM
18	D2O	100 %

Sample #6

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
19	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-10% 13C]	2.0 mM
20	KCl		100 mM
21	D2O		100 %

11 4D HC(CCO)NH-TOCSY

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
1	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-15N]	2.0 mM
2	KCl		100 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
5	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-95% 13C; U-98% 15N]	2.0 mM
6	KCl		100 mM
7	H2O		90 %
8	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
9	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-15N]-Lys	2.0 mM
10	KCl		100 mM
11	H2O		90 %
12	D2O		10 %

Sample #4

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
13	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-95% 13C; U-98% 15N]	2.0 mM
14	KCl		100 mM
15	D2O		100 %

Sample #5

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Concentration
16	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	2.0 mM
17	KCl	100 mM
18	D2O	100 %

Sample #6

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
19	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-10% 13C]	2.0 mM
20	KCl		100 mM
21	D2O		100 %

12 15N-HSQC

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
1	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-15N]	2.0 mM
2	KCl		100 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
5	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-95% 13C; U-98% 15N]	2.0 mM
6	KCl		100 mM
7	H2O		90 %
8	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
9	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-15N]-Lys	2.0 mM
10	KCl		100 mM
11	H2O		90 %
12	D2O		10 %

Sample #4

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
13	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-95% 13C; U-98% 15N]	2.0 mM
14	KCl		100 mM
15	D2O		100 %

Sample #5

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Concentration
16	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	2.0 mM
17	KCl	100 mM
18	D2O	100 %

Sample #6

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
19	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-10% 13C]	2.0 mM
20	KCl		100 mM
21	D2O		100 %

13 high-resolution 13C-HSQC

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
1	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-15N]	2.0 mM
2	KCl		100 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
5	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-95% 13C; U-98% 15N]	2.0 mM
6	KCl		100 mM
7	H2O		90 %
8	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
9	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-15N]-Lys	2.0 mM
10	KCl		100 mM
11	H2O		90 %
12	D2O		10 %

Sample #4

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
13	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-95% 13C; U-98% 15N]	2.0 mM
14	KCl		100 mM
15	D2O		100 %

Sample #5

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Concentration
16	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	2.0 mM
17	KCl	100 mM
18	D2O	100 %

Sample #6

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
19	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-10% 13C]	2.0 mM
20	KCl		100 mM
21	D2O		100 %

14 RDC experiment in phage

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
1	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-15N]	2.0 mM
2	KCl		100 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
5	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-95% 13C; U-98% 15N]	2.0 mM
6	KCl		100 mM
7	H2O		90 %
8	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
9	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-15N]-Lys	2.0 mM
10	KCl		100 mM
11	H2O		90 %
12	D2O		10 %

Sample #4

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
13	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-95% 13C; U-98% 15N]	2.0 mM
14	KCl		100 mM
15	D2O		100 %

Sample #5

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Concentration
16	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	2.0 mM
17	KCl	100 mM
18	D2O	100 %

Sample #6

Pressure 1 atm, Temperature 300 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
19	the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1	[U-10% 13C]	2.0 mM
20	KCl		100 mM
21	D2O		100 %

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr6834_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr6834_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	chemical_shift_set_1	Warning	1247		97.3 (chain: 1, length: 112)
1	Chemical shift references	chemical_shift_reference	OK	3		No information

Assigned chemical shifts

Saveframe: chemical_shift_set_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 112, Sequence coverage: 97.3%
- Total number of assignments
  - ¹³C chemical shifts: 381
  - ¹H chemical shifts: 756
  - ¹⁵N chemical shifts: 110
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Keywords hSet2, hSRI, PCAP, PCID, phosphoCTD associating protein, Set2, Set2 Rpb1-interacting domain, SRI, SRI domain

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	527	381	72.3
¹H chemical shifts	729	662	90.8
¹⁵N chemical shifts	120	110	91.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	224	109	48.7
¹H chemical shifts	224	215	96.0
¹⁵N chemical shifts	108	103	95.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	303	272	89.8
¹H chemical shifts	505	447	88.5
¹⁵N chemical shifts	12	7	58.3

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Found 1 Document (2.069 seconds)

BMRB: 6834

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles Swiss-Prot: 2 entries Detail

Related entities 1. Huntingtin interacting protein B isoform 1, : 1 : 5 : 5 entities Detail

Interaction partners 1. Huntingtin interacting protein B isoform 1, : 14 interactors Detail

Experiments performed 14 experiments Detail

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Sample #6

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Sample #1

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Sample #5

Sample #6

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Sample #4

Sample #5

Sample #6

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Sample #1

Sample #2

Sample #3

Sample #4