1H, 13C and 15N resonance assignment of M-crystallin: a novel Ca2+ binding protein of the bg-crystallin superfamily from Methanosarcina acetivorans
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 86.3 % (829 of 961) | 87.9 % (436 of 496) | 83.6 % (312 of 373) | 88.0 % (81 of 92) |
Backbone | 98.4 % (496 of 504) | 97.7 % (173 of 177) | 98.8 % (242 of 245) | 98.8 % (81 of 82) |
Sidechain | 76.5 % (407 of 532) | 82.4 % (263 of 319) | 70.9 % (144 of 203) | 0.0 % (0 of 10) |
Aromatic | 32.8 % (38 of 116) | 65.5 % (38 of 58) | 0.0 % (0 of 56) | 0.0 % (0 of 2) |
Methyl | 100.0 % (70 of 70) | 100.0 % (35 of 35) | 100.0 % (35 of 35) |
1. M-Crystallin
MNAAEVIVYE HVNFGGKSFD ATSDQPGAGD NLNDKISSIK VKSGTWRFYE YINYGGRYWD LGPGEYSSVE SAGIPDNSIS SFRQITemperature 298 (±1) K, pH 7.5 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | M-Crystallin | [U-99% 15N] | 1.1 mM | |
2 | CALCIUM (II) ION | 6.6 mM |
Temperature 298 (±1) K, pH 7.5 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
3 | M-Crystallin | [U-99% 13C; U-99% 15N] | 0.9 mM | |
4 | CALCIUM (II) ION | 5.4 mM |