1H, 13C, and 15N Resonance Assignments for the reduced form of Thioredoxin 1 from Sacharomyces cerevisae
MVTQFKTASE FDSAIAQDKL VVVDFYATWC GPCKMIAPMI EKFSEQYPQA DFYKLDVDEL GDVAQKNEVS AMPTLLLFKN GKEVAKVVGA NPAAIKQAIA ANA
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 92.8 % (1106 of 1192) | 93.3 % (573 of 614) | 91.3 % (428 of 469) | 96.3 % (105 of 109) |
Backbone | 96.2 % (585 of 608) | 97.6 % (200 of 205) | 95.4 % (291 of 305) | 95.9 % (94 of 98) |
Sidechain | 90.6 % (619 of 683) | 91.2 % (373 of 409) | 89.4 % (235 of 263) | 100.0 % (11 of 11) |
Aromatic | 91.7 % (88 of 96) | 95.8 % (46 of 48) | 87.2 % (41 of 47) | 100.0 % (1 of 1) |
Methyl | 100.0 % (124 of 124) | 100.0 % (62 of 62) | 100.0 % (62 of 62) |
1. Trx1
MVTQFKTASE FDSAIAQDKL VVVDFYATWC GPCKMIAPMI EKFSEQYPQA DFYKLDVDEL GDVAQKNEVS AMPTLLLFKN GKEVAKVVGA NPAAIKQAIA ANATemperature 308 (±0.2) K, pH 7.0 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | thioredoxin 2 | [U-13C; U-15N] | 1 mM | |
2 | 2-mercaptoethanol | 3 mM | ||
3 | H2O | 90 % | ||
4 | D2O | 10 % |