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BMRB: 6930

2K3K

NMR assignments of SNF RBD1

Authors

Liu, C., Cui, G., Jin, C., Xia, B.

Assembly

SNF RBD1

Entity

1. SNF RBD1 (polymer, Thiol state: not present), 104 monomers, 11954.97 Da Detail

MEMLPNQTIY INNLNEKIKK EELKKSLYAI FSQFGQILDI VALKTLKMRG QAFVIFKEIG SASNALRTMQ GFPFYDKPMQ IAYSKSDSDI VAKIKGTFKE RPKK

Formula weight

11954.97 Da

Source organism

Drosophila melanogaster

Exptl. method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.0 %, Completeness: 92.9 %, Completeness (bb): 97.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	92.9 % (1217 of 1310)	93.7 % (649 of 693)	90.7 % (459 of 506)	98.2 % (109 of 111)
Backbone	97.6 % (601 of 616)	98.6 % (206 of 209)	96.7 % (297 of 307)	98.0 % (98 of 100)
Sidechain	89.8 % (712 of 793)	91.5 % (443 of 484)	86.6 % (258 of 298)	100.0 % (11 of 11)
Aromatic	37.3 % (38 of 102)	47.1 % (24 of 51)	27.5 % (14 of 51)
Methyl	100.0 % (110 of 110)	100.0 % (55 of 55)	100.0 % (55 of 55)

1. snRNP protein

MEMLPNQTIY INNLNEKIKK EELKKSLYAI FSQFGQILDI VALKTLKMRG QAFVIFKEIG SASNALRTMQ GFPFYDKPMQ IAYSKSDSDI VAKIKGTFKE RPKK

Show sample condition

Sample

Temperature 298 K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	SNF RBD1	[U-15N; U-13C]		0.4 (±0.05) mM
2	PBS			50 mM
3	NaCl			50 mM
4	H2O			90 %
5	D2Ol			10 %

LACS Plot; CA

Referencing offset: -0.2 ppm, Outliers: 2 Detail

LACS Plot; CB

Referencing offset: -0.2 ppm, Outliers: 2 Detail

LACS Plot; HA

Referencing offset: -0.02 ppm, Outliers: 3 Detail

LACS Plot; CO

Referencing offset: 0.11 ppm, Outliers: 1 Detail

Release date

2009-10-19

Citation

Structure and novel functional mechanism of Drosophila SNF in sex-lethal splicing
Hu, J., Cui, G., Li, C., Liu, C., Shang, E., Lai, L., Jin, C., Wang, J., Xia, B.
PLoS One (2009), 4, e6890-e6890, PubMed 19727396 , DOI 10.1371/journal.pone.0006890 , Abstract

Related entities 1. SNF RBD1, : 1 : 2 : 4 : 244 entities Detail

Interaction partners 1. SNF RBD1, : 2 interactors Detail

More details for 2 interactors identified by 2 citations

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Temperature 298 K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	SNF RBD1	[U-15N; U-13C]		0.4 (±0.05) mM
2	PBS			50 mM
3	NaCl			50 mM
4	H2O			90 %
5	D2Ol			10 %

2 2D 1H-13C HSQC

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Temperature 298 K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	SNF RBD1	[U-15N; U-13C]		0.4 (±0.05) mM
2	PBS			50 mM
3	NaCl			50 mM
4	H2O			90 %
5	D2Ol			10 %

3 3D HNCACB

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Temperature 298 K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	SNF RBD1	[U-15N; U-13C]		0.4 (±0.05) mM
2	PBS			50 mM
3	NaCl			50 mM
4	H2O			90 %
5	D2Ol			10 %

4 3D CBCA(CO)NH

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Temperature 298 K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	SNF RBD1	[U-15N; U-13C]		0.4 (±0.05) mM
2	PBS			50 mM
3	NaCl			50 mM
4	H2O			90 %
5	D2Ol			10 %

5 3D HNCO

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Temperature 298 K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	SNF RBD1	[U-15N; U-13C]		0.4 (±0.05) mM
2	PBS			50 mM
3	NaCl			50 mM
4	H2O			90 %
5	D2Ol			10 %

6 3D HNCA

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Temperature 298 K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	SNF RBD1	[U-15N; U-13C]		0.4 (±0.05) mM
2	PBS			50 mM
3	NaCl			50 mM
4	H2O			90 %
5	D2Ol			10 %

7 3D HCCH-TOCSY

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Temperature 298 K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	SNF RBD1	[U-15N; U-13C]		0.4 (±0.05) mM
2	PBS			50 mM
3	NaCl			50 mM
4	H2O			90 %
5	D2Ol			10 %

8 3D HCCH-COSY

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Temperature 298 K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	SNF RBD1	[U-15N; U-13C]		0.4 (±0.05) mM
2	PBS			50 mM
3	NaCl			50 mM
4	H2O			90 %
5	D2Ol			10 %

9 3D (H)CCH-COSY

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Temperature 298 K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	SNF RBD1	[U-15N; U-13C]		0.4 (±0.05) mM
2	PBS			50 mM
3	NaCl			50 mM
4	H2O			90 %
5	D2Ol			10 %

10 3D (H)CCH-TOCSY

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Temperature 298 K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	SNF RBD1	[U-15N; U-13C]		0.4 (±0.05) mM
2	PBS			50 mM
3	NaCl			50 mM
4	H2O			90 %
5	D2Ol			10 %

11 3D 15N-separated NOESY

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Temperature 298 K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	SNF RBD1	[U-15N; U-13C]		0.4 (±0.05) mM
2	PBS			50 mM
3	NaCl			50 mM
4	H2O			90 %
5	D2Ol			10 %

12 3D 13C-separated NOESY

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Temperature 298 K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	SNF RBD1	[U-15N; U-13C]		0.4 (±0.05) mM
2	PBS			50 mM
3	NaCl			50 mM
4	H2O			90 %
5	D2Ol			10 %

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr6930_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr6930_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	chem_shift_list_1	Warning	1325		99.0 (chain: 1, length: 104)
1	Chemical shift references	chemical_shift_referencing	OK	3		No information

Assigned chemical shifts

Saveframe: chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 104, Sequence coverage: 99.0%
- Total number of assignments
  - ¹H chemical shifts: 762
  - ¹³C chemical shifts: 457
  - ¹⁵N chemical shifts: 106
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Keywords RNA binding protein, RNA splicing factor, SNF