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BMRB: 6936

3JQO

Resonance assignments for the pKM101 homologue of VirB7 (TraN) in complex with the pKM101 homologue of VirB9 (TraO)

Authors

Harris, R., Bayliss, R., Waksman, G., Driscoll, P.C.

Assembly

VirB7/VirB9

Entity

1. VirB7 (polymer, Thiol state: not present), 36 monomers, 3772.073 Da Detail

GAMASGHKPP PEPDWSNTVP VNKTIPVDTQ GGRNES

2. VirB9 (polymer, Thiol state: not present), 126 monomers, 14059.59 Da Detail

GIDPFTHMAG AKNYQYVMSE QPEMRSIQPV HVWDNYRFTR FEFPANAELP QVYMISASGK ETLPNSHVVG ENRNIIEVET VAKEWRIRLG DKVVGVRNNN FAPGAGAVAT GTASPDVRRV QIGEDN

Total weight

17831.662 Da

Max. entity weight

14059.59 Da

Source organism

Escherichia coli

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.2 %, Completeness: 90.7 %, Completeness (bb): 91.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	90.7 % (1660 of 1831)	90.8 % (867 of 955)	90.7 % (640 of 706)	90.0 % (153 of 170)
Backbone	91.1 % (860 of 944)	90.1 % (292 of 324)	91.9 % (434 of 472)	90.5 % (134 of 148)
Sidechain	90.6 % (938 of 1035)	91.1 % (575 of 631)	90.1 % (344 of 382)	86.4 % (19 of 22)
Aromatic	77.6 % (104 of 134)	85.1 % (57 of 67)	70.3 % (45 of 64)	66.7 % (2 of 3)
Methyl	96.2 % (150 of 156)	96.2 % (75 of 78)	96.2 % (75 of 78)

1. VirB7

GAMASGHKPP PEPDWSNTVP VNKTIPVDTQ GGRNES

2. VirB9

GIDPFTHMAG AKNYQYVMSE QPEMRSIQPV HVWDNYRFTR FEFPANAELP QVYMISASGK ETLPNSHVVG ENRNIIEVET VAKEWRIRLG DKVVGVRNNN FAPGAGAVAT GTASPDVRRV QIGEDN

Show sample condition

Sample #1

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
1	VirB7	[U-15N]		1 mM
2	VirB9			1 mM

Sample #2

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
5	VirB7			1 mM
6	VirB9	[U-15N]		1 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.08 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.08 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.62 ppm, Outliers: 2 Detail

Release date

2006-04-13

Citation

Resonance assignments of the complex between TraN and the C-terminal domain of TraO from the conjugative plasmid pKM101
Harris, R., Bayliss, R., Waksman, G., Driscoll, P.C.
J. Biomol. NMR (2006), 36 Suppl 1, 31-31, PubMed 16601857 , DOI 10.1007/s10858-006-0015-9 , Abstract

Related entities 1. VirB7, : 1 : 17 entities Detail

Related entities 2. VirB9, : 1 : 1 : 6 entities Detail

Experiments performed 16 experiments Detail

1 1H 15N HSQC

Instrument

#1 Model Varian Unity Plus (500 MHz)

#2 Model Varian Inova (600 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
1	VirB7	[U-15N]		1 mM
2	VirB9			1 mM

Sample #2

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
3	VirB7	[U-13C; U-15N]		1 mM
4	VirB9			1 mM

Sample #3

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
5	VirB7			1 mM
6	VirB9	[U-15N]		1 mM

Sample #4

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
7	VirB7			1 mM
8	VirB9	[U-13C; U-15N]		1 mM

2 1H 13C HSQC

Instrument

#1 Model Varian Unity Plus (500 MHz)

#2 Model Varian Inova (600 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
1	VirB7	[U-15N]		1 mM
2	VirB9			1 mM

Sample #2

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
3	VirB7	[U-13C; U-15N]		1 mM
4	VirB9			1 mM

Sample #3

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
5	VirB7			1 mM
6	VirB9	[U-15N]		1 mM

Sample #4

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
7	VirB7			1 mM
8	VirB9	[U-13C; U-15N]		1 mM

3 1H 13C CT-HSQC

Instrument

#1 Model Varian Unity Plus (500 MHz)

#2 Model Varian Inova (600 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
1	VirB7	[U-15N]		1 mM
2	VirB9			1 mM

Sample #2

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
3	VirB7	[U-13C; U-15N]		1 mM
4	VirB9			1 mM

Sample #3

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
5	VirB7			1 mM
6	VirB9	[U-15N]		1 mM

Sample #4

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
7	VirB7			1 mM
8	VirB9	[U-13C; U-15N]		1 mM

4 1H 15N TOCSY-HSQC

Instrument

#1 Model Varian Unity Plus (500 MHz)

#2 Model Varian Inova (600 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
1	VirB7	[U-15N]		1 mM
2	VirB9			1 mM

Sample #2

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
3	VirB7	[U-13C; U-15N]		1 mM
4	VirB9			1 mM

Sample #3

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
5	VirB7			1 mM
6	VirB9	[U-15N]		1 mM

Sample #4

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
7	VirB7			1 mM
8	VirB9	[U-13C; U-15N]		1 mM

5 1H 15N NOESY-HSQC

Instrument

#1 Model Varian Unity Plus (500 MHz)

#2 Model Varian Inova (600 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
1	VirB7	[U-15N]		1 mM
2	VirB9			1 mM

Sample #2

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
3	VirB7	[U-13C; U-15N]		1 mM
4	VirB9			1 mM

Sample #3

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
5	VirB7			1 mM
6	VirB9	[U-15N]		1 mM

Sample #4

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
7	VirB7			1 mM
8	VirB9	[U-13C; U-15N]		1 mM

6 HNCO

Instrument

#1 Model Varian Unity Plus (500 MHz)

#2 Model Varian Inova (600 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
1	VirB7	[U-15N]		1 mM
2	VirB9			1 mM

Sample #2

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
3	VirB7	[U-13C; U-15N]		1 mM
4	VirB9			1 mM

Sample #3

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
5	VirB7			1 mM
6	VirB9	[U-15N]		1 mM

Sample #4

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
7	VirB7			1 mM
8	VirB9	[U-13C; U-15N]		1 mM

7 HN(CA)CO

Instrument

#1 Model Varian Unity Plus (500 MHz)

#2 Model Varian Inova (600 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
1	VirB7	[U-15N]		1 mM
2	VirB9			1 mM

Sample #2

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
3	VirB7	[U-13C; U-15N]		1 mM
4	VirB9			1 mM

Sample #3

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
5	VirB7			1 mM
6	VirB9	[U-15N]		1 mM

Sample #4

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
7	VirB7			1 mM
8	VirB9	[U-13C; U-15N]		1 mM

8 HNCA

Instrument

#1 Model Varian Unity Plus (500 MHz)

#2 Model Varian Inova (600 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
1	VirB7	[U-15N]		1 mM
2	VirB9			1 mM

Sample #2

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
3	VirB7	[U-13C; U-15N]		1 mM
4	VirB9			1 mM

Sample #3

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
5	VirB7			1 mM
6	VirB9	[U-15N]		1 mM

Sample #4

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
7	VirB7			1 mM
8	VirB9	[U-13C; U-15N]		1 mM

9 HN(CO)CA

Instrument

#1 Model Varian Unity Plus (500 MHz)

#2 Model Varian Inova (600 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
1	VirB7	[U-15N]		1 mM
2	VirB9			1 mM

Sample #2

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
3	VirB7	[U-13C; U-15N]		1 mM
4	VirB9			1 mM

Sample #3

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
5	VirB7			1 mM
6	VirB9	[U-15N]		1 mM

Sample #4

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
7	VirB7			1 mM
8	VirB9	[U-13C; U-15N]		1 mM

10 HNCACB

Instrument

#1 Model Varian Unity Plus (500 MHz)

#2 Model Varian Inova (600 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
1	VirB7	[U-15N]		1 mM
2	VirB9			1 mM

Sample #2

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
3	VirB7	[U-13C; U-15N]		1 mM
4	VirB9			1 mM

Sample #3

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
5	VirB7			1 mM
6	VirB9	[U-15N]		1 mM

Sample #4

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
7	VirB7			1 mM
8	VirB9	[U-13C; U-15N]		1 mM

11 CBCA(CO)NH

Instrument

#1 Model Varian Unity Plus (500 MHz)

#2 Model Varian Inova (600 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
1	VirB7	[U-15N]		1 mM
2	VirB9			1 mM

Sample #2

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
3	VirB7	[U-13C; U-15N]		1 mM
4	VirB9			1 mM

Sample #3

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
5	VirB7			1 mM
6	VirB9	[U-15N]		1 mM

Sample #4

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
7	VirB7			1 mM
8	VirB9	[U-13C; U-15N]		1 mM

12 HA(CA)NH

Instrument

#1 Model Varian Unity Plus (500 MHz)

#2 Model Varian Inova (600 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
1	VirB7	[U-15N]		1 mM
2	VirB9			1 mM

Sample #2

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
3	VirB7	[U-13C; U-15N]		1 mM
4	VirB9			1 mM

Sample #3

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
5	VirB7			1 mM
6	VirB9	[U-15N]		1 mM

Sample #4

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
7	VirB7			1 mM
8	VirB9	[U-13C; U-15N]		1 mM

13 HA(CACO)NH

Instrument

#1 Model Varian Unity Plus (500 MHz)

#2 Model Varian Inova (600 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
1	VirB7	[U-15N]		1 mM
2	VirB9			1 mM

Sample #2

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
3	VirB7	[U-13C; U-15N]		1 mM
4	VirB9			1 mM

Sample #3

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
5	VirB7			1 mM
6	VirB9	[U-15N]		1 mM

Sample #4

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
7	VirB7			1 mM
8	VirB9	[U-13C; U-15N]		1 mM

14 HCCH-TOCSY

Instrument

#1 Model Varian Unity Plus (500 MHz)

#2 Model Varian Inova (600 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
1	VirB7	[U-15N]		1 mM
2	VirB9			1 mM

Sample #2

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
3	VirB7	[U-13C; U-15N]		1 mM
4	VirB9			1 mM

Sample #3

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
5	VirB7			1 mM
6	VirB9	[U-15N]		1 mM

Sample #4

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
7	VirB7			1 mM
8	VirB9	[U-13C; U-15N]		1 mM

15 HCCH-COSY

Instrument

#1 Model Varian Unity Plus (500 MHz)

#2 Model Varian Inova (600 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
1	VirB7	[U-15N]		1 mM
2	VirB9			1 mM

Sample #2

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
3	VirB7	[U-13C; U-15N]		1 mM
4	VirB9			1 mM

Sample #3

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
5	VirB7			1 mM
6	VirB9	[U-15N]		1 mM

Sample #4

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
7	VirB7			1 mM
8	VirB9	[U-13C; U-15N]		1 mM

16 1H 13C NOESY-HSQC

Instrument

#1 Model Varian Unity Plus (500 MHz)

#2 Model Varian Inova (600 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
1	VirB7	[U-15N]		1 mM
2	VirB9			1 mM

Sample #2

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
3	VirB7	[U-13C; U-15N]		1 mM
4	VirB9			1 mM

Sample #3

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
5	VirB7			1 mM
6	VirB9	[U-15N]		1 mM

Sample #4

Temperature 298 K, pH 6.7 (±0.1), Details Complex between VirB7 and the C-terminal domain of VirB9 of the homologues of pKM101

#	Name	Isotope labeling	Type	Concentration
7	VirB7			1 mM
8	VirB9	[U-13C; U-15N]		1 mM

Chemical shift validation 4 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr6936_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr6936_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	chem_shift_list_1	Warning	1801		88.9 (chain: 1, length: 36), 93.7 (chain: 2, length: 126)
1	Chemical shift references	chemical_shift_referencing	OK	3		No information

Assigned chemical shifts

Saveframe: chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 36, Sequence coverage: 88.9%
  - Entity_assembly_ID: 2, Chain length: 126, Sequence coverage: 93.7%
- Total number of assignments
  - ¹H chemical shifts: 1016
  - ¹³C chemical shifts: 634
  - ¹⁵N chemical shifts: 151
- Completeness of assignments
  - Entity_assemble_ID: 1
  - Entity_assemble_ID: 2
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1
  - Chain_ID: 2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	204	184	90.2
¹³C chemical shifts	148	131	88.5
¹⁵N chemical shifts	36	30	83.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	70	59	84.3
¹³C chemical shifts	72	60	83.3
¹⁵N chemical shifts	30	26	86.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	134	125	93.3
¹³C chemical shifts	76	71	93.4
¹⁵N chemical shifts	6	4	66.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	8	7	87.5
¹³C chemical shifts	7	7	100.0
¹⁵N chemical shifts	1	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	751	680	90.5
¹³C chemical shifts	558	503	90.1
¹⁵N chemical shifts	144	121	84.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	254	232	91.3
¹³C chemical shifts	252	234	92.9
¹⁵N chemical shifts	118	106	89.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	59	50	84.7
¹³C chemical shifts	57	38	66.7
¹⁵N chemical shifts	2	2	100.0

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Found 1 Document (1.145 seconds)

BMRB: 6936

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. VirB7, : 1 : 17 entities Detail

Related entities 2. VirB9, : 1 : 1 : 6 entities Detail

Experiments performed 16 experiments Detail

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