1H, 13C and 15N NMR assignment of the region 51-160 of human KIN17 protein
GQRQLLLASE NPQQFMDYFS EEFRNDFLEL LRRRFGTKRV HNNIVYNEYI SHREHIHMNA TQWETLTDFT KWLGREGLCK VDETPKGWYI QYIDRDPETI RRQLELEKKK K
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 97.1 % (1372 of 1413) | 96.3 % (721 of 749) | 98.3 % (531 of 540) | 96.8 % (120 of 124) |
Backbone | 98.6 % (651 of 660) | 97.8 % (219 of 224) | 99.7 % (327 of 328) | 97.2 % (105 of 108) |
Sidechain | 96.3 % (827 of 859) | 95.6 % (502 of 525) | 97.5 % (310 of 318) | 93.8 % (15 of 16) |
Aromatic | 90.8 % (138 of 152) | 92.1 % (70 of 76) | 89.0 % (65 of 73) | 100.0 % (3 of 3) |
Methyl | 99.0 % (97 of 98) | 98.0 % (48 of 49) | 100.0 % (49 of 49) |
1. K2
GQRQLLLASE NPQQFMDYFS EEFRNDFLEL LRRRFGTKRV HNNIVYNEYI SHREHIHMNA TQWETLTDFT KWLGREGLCK VDETPKGWYI QYIDRDPETI RRQLELEKKK KTemperature 303 (±0.2) K, pH 6.0 (±0.1), Details 15N label
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | KIN17 | [U-15N] | protein | 0.7 mM |
2 | NaCl | salt | 150 mM | |
3 | NaH2PO4/Na2HPO4 Phosphate | salt | 50 mM | |
4 | H2O | solvent | 90 % | |
5 | D2O | 10 % | ||
6 | TCEP | 1 mM |
Temperature 303 (±0.2) K, pH 6.0 (±0.1), Details 15N/13C label
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | KIN17 | [U-13C; U-15N] | protein | 0.8 mM |
8 | NaCl | salt | 150 mM | |
9 | NaH2PO4/Na2HPO4 Phosphate | salt | 50 mM | |
10 | H2O | solvent | 90 % | |
11 | D2O | 10 % | ||
12 | TCEP | 1 mM |
Temperature 303 (±0.2) K, pH 6.0 (±0.1), Details 15N/13C label in D2O 100%
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
13 | KIN17 | [U-13C; U-15N] | protein | 0.7 mM |
14 | NaCl | salt | 150 mM | |
15 | NaH2PO4/Na2HPO4 Phosphate | salt | 50 mM | |
16 | D2O | 100 % | ||
17 | TCEP | 1 mM |