NMR assignment of the a' domain of thermophilic fungal protein disulfide isomerase
GPLGSEGPVT VVVAKNYNEI VLDDTKDVLI EFYAPWCGHC KALAPKYEEL GALYAKSEFK DRVVIAKVDA TANDVPDEIQ GFPTIKLYPA GAKGQPVTYS GSRTVEDLIK FIAENGKYKA A
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 85.5 % (1182 of 1383) | 88.4 % (630 of 713) | 79.3 % (436 of 550) | 96.7 % (116 of 120) |
Backbone | 95.6 % (679 of 710) | 95.9 % (234 of 244) | 95.2 % (336 of 353) | 96.5 % (109 of 113) |
Sidechain | 77.9 % (611 of 784) | 84.4 % (396 of 469) | 67.5 % (208 of 308) | 100.0 % (7 of 7) |
Aromatic | 36.6 % (41 of 112) | 62.5 % (35 of 56) | 9.1 % (5 of 55) | 100.0 % (1 of 1) |
Methyl | 92.6 % (137 of 148) | 100.0 % (74 of 74) | 85.1 % (63 of 74) |
1. PDI-a'
GPLGSEGPVT VVVAKNYNEI VLDDTKDVLI EFYAPWCGHC KALAPKYEEL GALYAKSEFK DRVVIAKVDA TANDVPDEIQ GFPTIKLYPA GAKGQPVTYS GSRTVEDLIK FIAENGKYKA ATemperature 310 (±1) K, pH 6.0 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | PDI-a' | [U-13C; U-15N] | 1 mM |