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BMRB: 6990

2KQA

1H, 15N and 13C resonance assignments of the cerato-platanin, a phytotoxic protein from Ceratocystis fimbriata

Authors

Oliveira, A.L., Pazzagli, L., Cappugi, G., Benedetti, C.E., Spisni, A., Pertinhez, T.A.

Assembly

Cerato-platanin

Entity

1. Cerato-platanin (polymer, Thiol state: all disulfide bound), 129 monomers, 13427.77 Da Detail

EEGVSLEKRV SISYDPIYAA DLSMGSVACS NGDHGLMAQY PTLGEVPGFP NVGGIPDIAG WDSPSCGTCW KVTIPNGNSI FIRGVDSGRG GFNVNPTAFT KLVGSTEAGR VDNVNYVQVD LSNCINGAN

Formula weight

13427.77 Da

Entity Connection

disulfide 2 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS29:SG	1:CYS66:SG
2	disulfide	sing	1:CYS69:SG	1:CYS124:SG

Source organism

Ceratocystis platani

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 98.5 %, Completeness (bb): 98.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	98.5 % (1353 of 1374)	98.7 % (694 of 703)	97.8 % (523 of 535)	100.0 % (136 of 136)
Backbone	98.8 % (749 of 758)	98.9 % (265 of 268)	98.4 % (363 of 369)	100.0 % (121 of 121)
Sidechain	97.9 % (712 of 727)	98.6 % (429 of 435)	96.8 % (268 of 277)	100.0 % (15 of 15)
Aromatic	95.0 % (95 of 100)	98.0 % (49 of 50)	91.7 % (44 of 48)	100.0 % (2 of 2)
Methyl	100.0 % (136 of 136)	100.0 % (68 of 68)	100.0 % (68 of 68)

1. Cerato-platanin

EEGVSLEKRV SISYDPIYAA DLSMGSVACS NGDHGLMAQY PTLGEVPGFP NVGGIPDIAG WDSPSCGTCW KVTIPNGNSI FIRGVDSGRG GFNVNPTAFT KLVGSTEAGR VDNVNYVQVD LSNCINGAN

Show sample condition

Sample

Temperature 293 (±0.2) K, pH 5.8 (±0.2), Details 10 mM phosphate buffer pH 5.8, 0.05% NaN3

#	Name	Isotope labeling	Type	Concentration
1	Cerato-platanin	[U-13C; U-15N]	protein	0.7 (±0.05) mM
2	phosphate buffer			10 mM
3	NaN3			0.05 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.07 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.07 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.05 ppm, Outliers: 1 Detail

Release date

2006-08-06

Citation 1

Purification, characterization, and amino acid sequence of cerato-platanin, a new phytotoxic protein from Ceratocystis fimbriata f. sp. platani
Pazzagli, L., Cappugi, G., Manao, G., Camici, G., Santini, A., Scala, A.
J. Biol. Chem. (1999), 274, 24959-24964, PubMed 10455173 , DOI: , Abstract

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Citation 2

1H, 15N and 13C Resonance assignments of cerato-platanin, a phytotoxic protein from Ceratocystis fimbriata
Oliveira, A.L., Pazzagli, L., Pantera, B., Cappugi, G., Benedetti, C.E., Spisni, A., Pertinhez, T.A.
J. Biomol. NMR (2006), 36 Suppl 1, 50-50, PubMed 16819587 , DOI 10.1007/s10858-006-9023-z , Abstract

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Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: P81702 released on 1999-07-15
Title CEPL_CERFI Entity Cerato-platanin

Related entities 1. Cerato-platanin, : 1 : 1 : 8 entities Detail

Experiments performed 10 experiments Detail

1 15N-HSQC