BMRBj - BMREntryMaster

Found 1 Document (5.03 seconds)

BMRB: 7025

2RR3

1H, 13C, and 15N Resonance Assignments of the VAP-A: OSBP Complex

Authors

Furuita, K., Mishima, M., Kojima, C.

Assembly

VAP-A:OSBP Complex

Entity

1. vesicle-associated membrane protein-associated protein-A (polymer, Thiol state: all free), 131 monomers, 14811.87 Da Detail

GPLGSDHEQI LVLDPPTDLK FKGPFTDVVT TNLKLRNPSD RKVCFKVKTT APRRYCVRPN SGIIDPGSTV TVSVMLQPFD YDPNEKSKHK FMVQTIFAPP NTSDMEAVWK EAKPDELMDS KLRCVFEMPN E

2. oxisterol-binding protein (polymer, Thiol state: all free), 48 monomers, 5455.707 Da Detail

GPLGSDHWGK GDMSDEDDEN EFFDAPEIIT MPENLGHKRT GSHHHHHH

Total weight

20267.578 Da

Max. entity weight

14811.87 Da

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.4 %, Completeness: 85.9 %, Completeness (bb): 93.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	85.9 % (1788 of 2081)	88.3 % (963 of 1091)	81.6 % (665 of 815)	91.4 % (160 of 175)
Backbone	93.8 % (976 of 1040)	93.2 % (328 of 352)	94.1 % (495 of 526)	94.4 % (153 of 162)
Sidechain	80.4 % (972 of 1209)	85.9 % (635 of 739)	72.2 % (330 of 457)	53.8 % (7 of 13)
Aromatic	33.5 % (57 of 170)	58.8 % (50 of 85)	7.2 % (6 of 83)	50.0 % (1 of 2)
Methyl	97.3 % (146 of 150)	97.3 % (73 of 75)	97.3 % (73 of 75)

1. vesicle-associated membrane protein-associated protein-A

GPLGSDHEQI LVLDPPTDLK FKGPFTDVVT TNLKLRNPSD RKVCFKVKTT APRRYCVRPN SGIIDPGSTV TVSVMLQPFD YDPNEKSKHK FMVQTIFAPP NTSDMEAVWK EAKPDELMDS KLRCVFEMPN E

2. oxisterol-binding protein

GPLGSDHWGK GDMSDEDDEN EFFDAPEIIT MPENLGHKRT GSHHHHHH

Show sample condition

Sample #1

Temperature 303 (±0.5) K, pH 6.9 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	vesicle-associated membrane protein-associated protein-A	[U-13C; U-15N]	protein	1.0 (±0.2) mM
2	oxisterol-binding protein		protein	1.0 (±0.2) mM
3	K phosphate		buffer	50 mM
4	KCl		salt	100 mM
5	DTT		reducing agent	1 mM
6	EDTA		chelating agent	0.1 mM

Sample #2

Temperature 303 (±0.5) K, pH 6.9 (±0.05)

#	Name	Isotope labeling	Type	Concentration
13	vesicle-associated membrane protein-associated protein-A	[U-15N]	protein	1.0 (±0.2) mM
14	oxisterol-binding protein	[U-15N]	protein	1.0 (±0.2) mM
15	K phosphate		buffer	50 mM
16	KCl		salt	100 mM
17	DTT		reducing agent	1 mM
18	EDTA		chelating agent	0.1 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.12 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.12 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.1 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.19 ppm, Outliers: 2 Detail

Release date

2006-10-01

Citation

1H, 13C and 15N resonance assignments of the VAP-A: OSBP complex
Furuita, K., Mishima, M., Kojima, C.
J. Biomol. NMR (2006), 36 Suppl 1, 69-69, PubMed 17031525 , DOI 10.1007/s10858-006-9057-2 , Abstract

Related entities 1. vesicle-associated membrane protein-associated protein-A, : 86 entities Detail

Related entities 2. oxisterol-binding protein, : 1 entities Detail

Interaction partners 1. vesicle-associated membrane protein-associated protein-A, : 92 interactors Detail

More details for 92 interactors identified by 39 citations

Experiments performed 11 experiments Detail

1 HNCACB

Instrument

#1 Model Bruker AVANCE (500 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Temperature 303 (±0.5) K, pH 6.9 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	vesicle-associated membrane protein-associated protein-A	[U-13C; U-15N]	protein	1.0 (±0.2) mM
2	oxisterol-binding protein		protein	1.0 (±0.2) mM
3	K phosphate		buffer	50 mM
4	KCl		salt	100 mM
5	DTT		reducing agent	1 mM
6	EDTA		chelating agent	0.1 mM

Sample #2

Temperature 303 (±0.5) K, pH 6.9 (±0.05)

#	Name	Isotope labeling	Type	Concentration
7	vesicle-associated membrane protein-associated protein-A		protein	1.0 (±0.2) mM
8	oxisterol-binding protein	[U-13C; U15N]	protein	1.0 (±0.2) mM
9	K phosphate		buffer	50 mM
10	KCl		salt	100 mM
11	DTT		reducing agent	1 mM
12	EDTA		chelating agent	0.1 mM

Sample #3

Temperature 303 (±0.5) K, pH 6.9 (±0.05)

#	Name	Isotope labeling	Type	Concentration
13	vesicle-associated membrane protein-associated protein-A	[U-15N]	protein	1.0 (±0.2) mM
14	oxisterol-binding protein	[U-15N]	protein	1.0 (±0.2) mM
15	K phosphate		buffer	50 mM
16	KCl		salt	100 mM
17	DTT		reducing agent	1 mM
18	EDTA		chelating agent	0.1 mM

2 HN(CO)CACB

Instrument

#1 Model Bruker AVANCE (500 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Temperature 303 (±0.5) K, pH 6.9 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	vesicle-associated membrane protein-associated protein-A	[U-13C; U-15N]	protein	1.0 (±0.2) mM
2	oxisterol-binding protein		protein	1.0 (±0.2) mM
3	K phosphate		buffer	50 mM
4	KCl		salt	100 mM
5	DTT		reducing agent	1 mM
6	EDTA		chelating agent	0.1 mM

Sample #2

Temperature 303 (±0.5) K, pH 6.9 (±0.05)

#	Name	Isotope labeling	Type	Concentration
7	vesicle-associated membrane protein-associated protein-A		protein	1.0 (±0.2) mM
8	oxisterol-binding protein	[U-13C; U15N]	protein	1.0 (±0.2) mM
9	K phosphate		buffer	50 mM
10	KCl		salt	100 mM
11	DTT		reducing agent	1 mM
12	EDTA		chelating agent	0.1 mM

Sample #3

Temperature 303 (±0.5) K, pH 6.9 (±0.05)

#	Name	Isotope labeling	Type	Concentration
13	vesicle-associated membrane protein-associated protein-A	[U-15N]	protein	1.0 (±0.2) mM
14	oxisterol-binding protein	[U-15N]	protein	1.0 (±0.2) mM
15	K phosphate		buffer	50 mM
16	KCl		salt	100 mM
17	DTT		reducing agent	1 mM
18	EDTA		chelating agent	0.1 mM

3 HN(CA)CO

Instrument

#1 Model Bruker AVANCE (500 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Temperature 303 (±0.5) K, pH 6.9 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	vesicle-associated membrane protein-associated protein-A	[U-13C; U-15N]	protein	1.0 (±0.2) mM
2	oxisterol-binding protein		protein	1.0 (±0.2) mM
3	K phosphate		buffer	50 mM
4	KCl		salt	100 mM
5	DTT		reducing agent	1 mM
6	EDTA		chelating agent	0.1 mM

Sample #2

Temperature 303 (±0.5) K, pH 6.9 (±0.05)

#	Name	Isotope labeling	Type	Concentration
7	vesicle-associated membrane protein-associated protein-A		protein	1.0 (±0.2) mM
8	oxisterol-binding protein	[U-13C; U15N]	protein	1.0 (±0.2) mM
9	K phosphate		buffer	50 mM
10	KCl		salt	100 mM
11	DTT		reducing agent	1 mM
12	EDTA		chelating agent	0.1 mM

Sample #3

Temperature 303 (±0.5) K, pH 6.9 (±0.05)

#	Name	Isotope labeling	Type	Concentration
13	vesicle-associated membrane protein-associated protein-A	[U-15N]	protein	1.0 (±0.2) mM
14	oxisterol-binding protein	[U-15N]	protein	1.0 (±0.2) mM
15	K phosphate		buffer	50 mM
16	KCl		salt	100 mM
17	DTT		reducing agent	1 mM
18	EDTA		chelating agent	0.1 mM

4 HNCO

Instrument

#1 Model Bruker AVANCE (500 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Temperature 303 (±0.5) K, pH 6.9 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	vesicle-associated membrane protein-associated protein-A	[U-13C; U-15N]	protein	1.0 (±0.2) mM
2	oxisterol-binding protein		protein	1.0 (±0.2) mM
3	K phosphate		buffer	50 mM
4	KCl		salt	100 mM
5	DTT		reducing agent	1 mM
6	EDTA		chelating agent	0.1 mM

Sample #2

Temperature 303 (±0.5) K, pH 6.9 (±0.05)

#	Name	Isotope labeling	Type	Concentration
7	vesicle-associated membrane protein-associated protein-A		protein	1.0 (±0.2) mM
8	oxisterol-binding protein	[U-13C; U15N]	protein	1.0 (±0.2) mM
9	K phosphate		buffer	50 mM
10	KCl		salt	100 mM
11	DTT		reducing agent	1 mM
12	EDTA		chelating agent	0.1 mM

Sample #3

Temperature 303 (±0.5) K, pH 6.9 (±0.05)

#	Name	Isotope labeling	Type	Concentration
13	vesicle-associated membrane protein-associated protein-A	[U-15N]	protein	1.0 (±0.2) mM
14	oxisterol-binding protein	[U-15N]	protein	1.0 (±0.2) mM
15	K phosphate		buffer	50 mM
16	KCl		salt	100 mM
17	DTT		reducing agent	1 mM
18	EDTA		chelating agent	0.1 mM

5 H(CCO)NH

Instrument

#1 Model Bruker AVANCE (500 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Temperature 303 (±0.5) K, pH 6.9 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	vesicle-associated membrane protein-associated protein-A	[U-13C; U-15N]	protein	1.0 (±0.2) mM
2	oxisterol-binding protein		protein	1.0 (±0.2) mM
3	K phosphate		buffer	50 mM
4	KCl		salt	100 mM
5	DTT		reducing agent	1 mM
6	EDTA		chelating agent	0.1 mM

Sample #2

Temperature 303 (±0.5) K, pH 6.9 (±0.05)

#	Name	Isotope labeling	Type	Concentration
7	vesicle-associated membrane protein-associated protein-A		protein	1.0 (±0.2) mM
8	oxisterol-binding protein	[U-13C; U15N]	protein	1.0 (±0.2) mM
9	K phosphate		buffer	50 mM
10	KCl		salt	100 mM
11	DTT		reducing agent	1 mM
12	EDTA		chelating agent	0.1 mM

Sample #3

Temperature 303 (±0.5) K, pH 6.9 (±0.05)

#	Name	Isotope labeling	Type	Concentration
13	vesicle-associated membrane protein-associated protein-A	[U-15N]	protein	1.0 (±0.2) mM
14	oxisterol-binding protein	[U-15N]	protein	1.0 (±0.2) mM
15	K phosphate		buffer	50 mM
16	KCl		salt	100 mM
17	DTT		reducing agent	1 mM
18	EDTA		chelating agent	0.1 mM

6 C(CO)NH

Instrument

#1 Model Bruker AVANCE (500 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Temperature 303 (±0.5) K, pH 6.9 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	vesicle-associated membrane protein-associated protein-A	[U-13C; U-15N]	protein	1.0 (±0.2) mM
2	oxisterol-binding protein		protein	1.0 (±0.2) mM
3	K phosphate		buffer	50 mM
4	KCl		salt	100 mM
5	DTT		reducing agent	1 mM
6	EDTA		chelating agent	0.1 mM

Sample #2

Temperature 303 (±0.5) K, pH 6.9 (±0.05)

#	Name	Isotope labeling	Type	Concentration
7	vesicle-associated membrane protein-associated protein-A		protein	1.0 (±0.2) mM
8	oxisterol-binding protein	[U-13C; U15N]	protein	1.0 (±0.2) mM
9	K phosphate		buffer	50 mM
10	KCl		salt	100 mM
11	DTT		reducing agent	1 mM
12	EDTA		chelating agent	0.1 mM

Sample #3

Temperature 303 (±0.5) K, pH 6.9 (±0.05)

#	Name	Isotope labeling	Type	Concentration
13	vesicle-associated membrane protein-associated protein-A	[U-15N]	protein	1.0 (±0.2) mM
14	oxisterol-binding protein	[U-15N]	protein	1.0 (±0.2) mM
15	K phosphate		buffer	50 mM
16	KCl		salt	100 mM
17	DTT		reducing agent	1 mM
18	EDTA		chelating agent	0.1 mM

7 HC(CO)NH

Instrument

#1 Model Bruker AVANCE (500 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Temperature 303 (±0.5) K, pH 6.9 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	vesicle-associated membrane protein-associated protein-A	[U-13C; U-15N]	protein	1.0 (±0.2) mM
2	oxisterol-binding protein		protein	1.0 (±0.2) mM
3	K phosphate		buffer	50 mM
4	KCl		salt	100 mM
5	DTT		reducing agent	1 mM
6	EDTA		chelating agent	0.1 mM

Sample #2

Temperature 303 (±0.5) K, pH 6.9 (±0.05)

#	Name	Isotope labeling	Type	Concentration
7	vesicle-associated membrane protein-associated protein-A		protein	1.0 (±0.2) mM
8	oxisterol-binding protein	[U-13C; U15N]	protein	1.0 (±0.2) mM
9	K phosphate		buffer	50 mM
10	KCl		salt	100 mM
11	DTT		reducing agent	1 mM
12	EDTA		chelating agent	0.1 mM

Sample #3

Temperature 303 (±0.5) K, pH 6.9 (±0.05)

#	Name	Isotope labeling	Type	Concentration
13	vesicle-associated membrane protein-associated protein-A	[U-15N]	protein	1.0 (±0.2) mM
14	oxisterol-binding protein	[U-15N]	protein	1.0 (±0.2) mM
15	K phosphate		buffer	50 mM
16	KCl		salt	100 mM
17	DTT		reducing agent	1 mM
18	EDTA		chelating agent	0.1 mM

8 HCCH_TOCSY

Instrument

#1 Model Bruker AVANCE (500 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Temperature 303 (±0.5) K, pH 6.9 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	vesicle-associated membrane protein-associated protein-A	[U-13C; U-15N]	protein	1.0 (±0.2) mM
2	oxisterol-binding protein		protein	1.0 (±0.2) mM
3	K phosphate		buffer	50 mM
4	KCl		salt	100 mM
5	DTT		reducing agent	1 mM
6	EDTA		chelating agent	0.1 mM

Sample #2

Temperature 303 (±0.5) K, pH 6.9 (±0.05)

#	Name	Isotope labeling	Type	Concentration
7	vesicle-associated membrane protein-associated protein-A		protein	1.0 (±0.2) mM
8	oxisterol-binding protein	[U-13C; U15N]	protein	1.0 (±0.2) mM
9	K phosphate		buffer	50 mM
10	KCl		salt	100 mM
11	DTT		reducing agent	1 mM
12	EDTA		chelating agent	0.1 mM

Sample #3

Temperature 303 (±0.5) K, pH 6.9 (±0.05)

#	Name	Isotope labeling	Type	Concentration
13	vesicle-associated membrane protein-associated protein-A	[U-15N]	protein	1.0 (±0.2) mM
14	oxisterol-binding protein	[U-15N]	protein	1.0 (±0.2) mM
15	K phosphate		buffer	50 mM
16	KCl		salt	100 mM
17	DTT		reducing agent	1 mM
18	EDTA		chelating agent	0.1 mM

9 15N-edited TOCSY-HSQC

Instrument

#1 Model Bruker AVANCE (500 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Temperature 303 (±0.5) K, pH 6.9 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	vesicle-associated membrane protein-associated protein-A	[U-13C; U-15N]	protein	1.0 (±0.2) mM
2	oxisterol-binding protein		protein	1.0 (±0.2) mM
3	K phosphate		buffer	50 mM
4	KCl		salt	100 mM
5	DTT		reducing agent	1 mM
6	EDTA		chelating agent	0.1 mM

Sample #2

Temperature 303 (±0.5) K, pH 6.9 (±0.05)

#	Name	Isotope labeling	Type	Concentration
7	vesicle-associated membrane protein-associated protein-A		protein	1.0 (±0.2) mM
8	oxisterol-binding protein	[U-13C; U15N]	protein	1.0 (±0.2) mM
9	K phosphate		buffer	50 mM
10	KCl		salt	100 mM
11	DTT		reducing agent	1 mM
12	EDTA		chelating agent	0.1 mM

Sample #3

Temperature 303 (±0.5) K, pH 6.9 (±0.05)

#	Name	Isotope labeling	Type	Concentration
13	vesicle-associated membrane protein-associated protein-A	[U-15N]	protein	1.0 (±0.2) mM
14	oxisterol-binding protein	[U-15N]	protein	1.0 (±0.2) mM
15	K phosphate		buffer	50 mM
16	KCl		salt	100 mM
17	DTT		reducing agent	1 mM
18	EDTA		chelating agent	0.1 mM

10 15N-filtered 2D-NOESY

Instrument

#1 Model Bruker AVANCE (500 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Temperature 303 (±0.5) K, pH 6.9 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	vesicle-associated membrane protein-associated protein-A	[U-13C; U-15N]	protein	1.0 (±0.2) mM
2	oxisterol-binding protein		protein	1.0 (±0.2) mM
3	K phosphate		buffer	50 mM
4	KCl		salt	100 mM
5	DTT		reducing agent	1 mM
6	EDTA		chelating agent	0.1 mM

Sample #2

Temperature 303 (±0.5) K, pH 6.9 (±0.05)

#	Name	Isotope labeling	Type	Concentration
7	vesicle-associated membrane protein-associated protein-A		protein	1.0 (±0.2) mM
8	oxisterol-binding protein	[U-13C; U15N]	protein	1.0 (±0.2) mM
9	K phosphate		buffer	50 mM
10	KCl		salt	100 mM
11	DTT		reducing agent	1 mM
12	EDTA		chelating agent	0.1 mM

Sample #3

Temperature 303 (±0.5) K, pH 6.9 (±0.05)

#	Name	Isotope labeling	Type	Concentration
13	vesicle-associated membrane protein-associated protein-A	[U-15N]	protein	1.0 (±0.2) mM
14	oxisterol-binding protein	[U-15N]	protein	1.0 (±0.2) mM
15	K phosphate		buffer	50 mM
16	KCl		salt	100 mM
17	DTT		reducing agent	1 mM
18	EDTA		chelating agent	0.1 mM

11 1H15N HSQC

Instrument

#1 Model Bruker AVANCE (500 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Temperature 303 (±0.5) K, pH 6.9 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	vesicle-associated membrane protein-associated protein-A	[U-13C; U-15N]	protein	1.0 (±0.2) mM
2	oxisterol-binding protein		protein	1.0 (±0.2) mM
3	K phosphate		buffer	50 mM
4	KCl		salt	100 mM
5	DTT		reducing agent	1 mM
6	EDTA		chelating agent	0.1 mM

Sample #2

Temperature 303 (±0.5) K, pH 6.9 (±0.05)

#	Name	Isotope labeling	Type	Concentration
7	vesicle-associated membrane protein-associated protein-A		protein	1.0 (±0.2) mM
8	oxisterol-binding protein	[U-13C; U15N]	protein	1.0 (±0.2) mM
9	K phosphate		buffer	50 mM
10	KCl		salt	100 mM
11	DTT		reducing agent	1 mM
12	EDTA		chelating agent	0.1 mM

Sample #3

Temperature 303 (±0.5) K, pH 6.9 (±0.05)

#	Name	Isotope labeling	Type	Concentration
13	vesicle-associated membrane protein-associated protein-A	[U-15N]	protein	1.0 (±0.2) mM
14	oxisterol-binding protein	[U-15N]	protein	1.0 (±0.2) mM
15	K phosphate		buffer	50 mM
16	KCl		salt	100 mM
17	DTT		reducing agent	1 mM
18	EDTA		chelating agent	0.1 mM

Chemical shift validation 4 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr7025_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr7025_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	chem_shift_list_1	Warning	1909		97.7 (chain: 1, length: 131), 85.4 (chain: 2, length: 48)
1	Chemical shift references	chemical_shift_referencing	OK	3		No information

Assigned chemical shifts

Saveframe: chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 131, Sequence coverage: 97.7%
  - Entity_assembly_ID: 2, Chain length: 48, Sequence coverage: 85.4%
- Total number of assignments
  - ¹H chemical shifts: 1097
  - ¹³C chemical shifts: 652
  - ¹⁵N chemical shifts: 160
- Completeness of assignments
  - Entity_assemble_ID: 1
  - Entity_assemble_ID: 2
- Redox state of CYS
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1
  - Chain_ID: 2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	816	732	89.7
¹³C chemical shifts	609	503	82.6
¹⁵N chemical shifts	133	119	89.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	253	246	97.2
¹³C chemical shifts	262	253	96.6
¹⁵N chemical shifts	117	115	98.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	563	486	86.3
¹³C chemical shifts	347	250	72.0
¹⁵N chemical shifts	16	4	25.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	275	219	79.6
¹³C chemical shifts	206	149	72.3
¹⁵N chemical shifts	49	41	83.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	99	84	84.8
¹³C chemical shifts	96	82	85.4
¹⁵N chemical shifts	45	38	84.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	53	40	75.5
¹³C chemical shifts	52	0	0.0
¹⁵N chemical shifts	1	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	176	135	76.7
¹³C chemical shifts	110	67	60.9
¹⁵N chemical shifts	4	3	75.0

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (5.03 seconds)

BMRB: 7025

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. vesicle-associated membrane protein-associated protein-A, : 86 entities Detail

Related entities 2. oxisterol-binding protein, : 1 entities Detail

Interaction partners 1. vesicle-associated membrane protein-associated protein-A, : 92 interactors Detail

Experiments performed 11 experiments Detail

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Chemical shift validation 4 contents Detail