BMRB: 7028

Chemical shifts of the selected resonances in calmodulin alone and in complex with various sk-MLCK target peptides

Authors

Findlay, W.A., Gradwell, M.J., Bayley, P.M.

Assembly

CaM-WFF

Entity

1. calmodulin (polymer, Thiol state: unknown), 148 monomers, 16725.22 Da Detail

ADQLTEEQIA EFYEAFSLFD KDGDGTITTK ELGTVMRSLG QNPTEAELQD MINEVDADGN GTIDFPEFLT MMARKMKDTD SEEEIREAFR VFDKDGNGFI SAAELRHVMT NLGEKLTDEE VDEMIREADI DGDGQVNYEE FVQMMTAK

2. WFF peptide (polymer, Thiol state: not present), 18 monomers, 2220.622 Da Detail

KRRWKKNFIA VSAANRFK

Total weight

18945.844 Da

Max. entity weight

16725.22 Da

Source organism

Drosophila melanogaster

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 7.8 %, Completeness: 2.8 %, Completeness (bb): 3.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	2.8 % (28 of 998)	2.8 % (28 of 998)
Backbone	3.8 % (13 of 341)	3.8 % (13 of 341)
Sidechain	2.3 % (15 of 657)	2.3 % (15 of 657)
Aromatic	5.6 % (4 of 71)	5.6 % (4 of 71)
Methyl	5.1 % (4 of 78)	5.1 % (4 of 78)

1. calmodulin

ADQLTEEQIA EFYEAFSLFD KDGDGTITTK ELGTVMRSLG QNPTEAELQD MINEVDADGN GTIDFPEFLT MMARKMKDTD SEEEIREAFR VFDKDGNGFI SAAELRHVMT NLGEKLTDEE VDEMIREADI DGDGQVNYEE FVQMMTAK

2. WFF peptide

KRRWKKNFIA VSAANRFK

Show sample condition

Sample

Temperature 303 (±0) K

#	Name	Isotope labeling	Type	Concentration
1	calmodulin			1 mM
2	Tris			10 mM
3	KCl			100 mM
4	CaCl2			10 mM
5	WFF peptide			1 mM

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Release date

2013-02-14

Citation

Role of the N-terminal region of the skeletal muscle myosin light chain kinase target sequence in its interaction with calmodulin
Findlay, W.A., Gradwell, M.J., Bayley, P.M.
Protein Sci. (1995), 4, 2375-2382, PubMed 8563635 , DOI 10.1002/pro.5560041116 , Abstract

Entries sharing articles BMRB: 4 entries Detail

Related entities 1. calmodulin, : 1 : 94 entities Detail

Related entities 2. WFF peptide, : 1 : 20 : 11 entities Detail

Interaction partners 2. WFF peptide, : 23 interactors Detail

Experiments performed 1 experiments Detail

Chemical shift validation 4 contents Detail