BMRBj - BMREntryMaster

Found 1 Document (1.656 seconds)

BMRB: 7069

2G9O

Solution structure and intermolecular interactions of the apo form of third metal-binding domain of ATP7A, the menkes disease protein

Authors

Banci, L., Bertini, I., Cantini, F., DellaMalva, N., Rosato, A., Herrmann, T., Wuthrich, K.

Assembly

Copper-transporting ATPase 1 (E.C.3.6.3.4)

Entity

1. Copper-transporting ATPase 1 (E.C.3.6.3.4) (polymer, Thiol state: all free), 90 monomers, 9859.925 Da Detail

NDSTATFIID GMHCKSCVSN IESTLSALQY VSSIVVSLEN RSAIVVYNAS SVTPESLRKA IEAVSPGLYR VSITSEVEIE GRLEHHHHHH

Formula weight

9859.925 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

Torsion angle dynamics coupled with simulated annealing followed by restrained energy minimization

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.1 %, Completeness: 78.9 %, Completeness (bb): 75.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	78.9 % (781 of 990)	87.1 % (438 of 503)	66.8 % (263 of 394)	86.0 % (80 of 93)
Backbone	75.7 % (406 of 536)	88.4 % (160 of 181)	63.3 % (169 of 267)	87.5 % (77 of 88)
Sidechain	83.7 % (453 of 541)	86.3 % (278 of 322)	80.4 % (172 of 214)	60.0 % (3 of 5)
Aromatic	33.9 % (21 of 62)	61.3 % (19 of 31)	6.5 % (2 of 31)
Methyl	98.3 % (116 of 118)	98.3 % (58 of 59)	98.3 % (58 of 59)

1. Copper-transporting ATPase 1 (E.C.3.6.3.4)

NDSTATFIID GMHCKSCVSN IESTLSALQY VSSIVVSLEN RSAIVVYNAS SVTPESLRKA IEAVSPGLYR VSITSEVEIE GRLEHHHHHH

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Concentration
1	Copper-transporting ATPase 1 (E.C.3.6.3.4)	1 mM
2	phosphate_buffer	100 mM
3	H2O	90 %
4	D2O	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	dioxane	methyl carbons	69.4 ppm	null	direct	1.0
¹H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.0

Referencing offset: 0.0 ppm, Outliers: 5 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	dioxane	methyl carbons	69.4 ppm	null	direct	1.0
¹H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.0

Referencing offset: 0.0 ppm, Outliers: 5 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	dioxane	methyl carbons	69.4 ppm	null	direct	1.0
¹H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.0

Referencing offset: -0.07 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 30 models in PDB: 2G9O, Strand ID: A Detail

Release date

2006-09-06

Citation

Solution structure and intermolecular interactions of the third metal-binding domain of ATP7A, the Menkes disease protein
Banci, L., Bertini, I., Cantini, F., DellaMalva, N., Herrmann, T., Rosato, A., Wuthrich, K.
J. Biol. Chem. (2006), 281, 29141-29147, PubMed 16873374 , DOI 10.1074/jbc.M603176200 , Abstract

Related entities 1. Copper-transporting ATPase 1 (E.C.3.6.3.4), : 1 : 1 : 2 : 64 entities Detail

Interaction partners 1. Copper-transporting ATPase 1 (E.C.3.6.3.4), : 5 interactors Detail

More details for 5 interactors identified by 3 citations

Experiments performed 7 experiments Detail

1 2D NOESY

Instrument

Model Bruker AVANCE (800 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Concentration
1	Copper-transporting ATPase 1 (E.C.3.6.3.4)	1 mM
2	phosphate_buffer	100 mM
3	H2O	90 %
4	D2O	10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
5	Copper-transporting ATPase 1 (E.C.3.6.3.4)	[U-15N]	1 mM
6	phosphate_buffer		100 mM
7	DTT		1 mM
8	H2O		90 %
9	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
10	Copper-transporting ATPase 1 (E.C.3.6.3.4)	[U-15N; U-13C]	1 mM
11	phosphate_buffer		100 mM
12	DTT		1 mM
13	H2O		90 %
14	D2O		10 %

2 2D TOCSY

Instrument

Model Bruker AVANCE (800 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Concentration
1	Copper-transporting ATPase 1 (E.C.3.6.3.4)	1 mM
2	phosphate_buffer	100 mM
3	H2O	90 %
4	D2O	10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
5	Copper-transporting ATPase 1 (E.C.3.6.3.4)	[U-15N]	1 mM
6	phosphate_buffer		100 mM
7	DTT		1 mM
8	H2O		90 %
9	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
10	Copper-transporting ATPase 1 (E.C.3.6.3.4)	[U-15N; U-13C]	1 mM
11	phosphate_buffer		100 mM
12	DTT		1 mM
13	H2O		90 %
14	D2O		10 %

3 3D 15N-separated NOESY

Instrument

Model Bruker AVANCE (800 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Concentration
1	Copper-transporting ATPase 1 (E.C.3.6.3.4)	1 mM
2	phosphate_buffer	100 mM
3	H2O	90 %
4	D2O	10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
5	Copper-transporting ATPase 1 (E.C.3.6.3.4)	[U-15N]	1 mM
6	phosphate_buffer		100 mM
7	DTT		1 mM
8	H2O		90 %
9	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
10	Copper-transporting ATPase 1 (E.C.3.6.3.4)	[U-15N; U-13C]	1 mM
11	phosphate_buffer		100 mM
12	DTT		1 mM
13	H2O		90 %
14	D2O		10 %

4 (H)CCH-TOCSY

Instrument

Model Bruker AVANCE (800 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Concentration
1	Copper-transporting ATPase 1 (E.C.3.6.3.4)	1 mM
2	phosphate_buffer	100 mM
3	H2O	90 %
4	D2O	10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
5	Copper-transporting ATPase 1 (E.C.3.6.3.4)	[U-15N]	1 mM
6	phosphate_buffer		100 mM
7	DTT		1 mM
8	H2O		90 %
9	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
10	Copper-transporting ATPase 1 (E.C.3.6.3.4)	[U-15N; U-13C]	1 mM
11	phosphate_buffer		100 mM
12	DTT		1 mM
13	H2O		90 %
14	D2O		10 %

5 CBCA(CO)NH

Instrument

Model Bruker AVANCE (800 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Concentration
1	Copper-transporting ATPase 1 (E.C.3.6.3.4)	1 mM
2	phosphate_buffer	100 mM
3	H2O	90 %
4	D2O	10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
5	Copper-transporting ATPase 1 (E.C.3.6.3.4)	[U-15N]	1 mM
6	phosphate_buffer		100 mM
7	DTT		1 mM
8	H2O		90 %
9	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
10	Copper-transporting ATPase 1 (E.C.3.6.3.4)	[U-15N; U-13C]	1 mM
11	phosphate_buffer		100 mM
12	DTT		1 mM
13	H2O		90 %
14	D2O		10 %

6 CBCANH

Instrument

Model Bruker AVANCE (800 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Concentration
1	Copper-transporting ATPase 1 (E.C.3.6.3.4)	1 mM
2	phosphate_buffer	100 mM
3	H2O	90 %
4	D2O	10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
5	Copper-transporting ATPase 1 (E.C.3.6.3.4)	[U-15N]	1 mM
6	phosphate_buffer		100 mM
7	DTT		1 mM
8	H2O		90 %
9	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
10	Copper-transporting ATPase 1 (E.C.3.6.3.4)	[U-15N; U-13C]	1 mM
11	phosphate_buffer		100 mM
12	DTT		1 mM
13	H2O		90 %
14	D2O		10 %

7 3D 13C-separated NOESY

Instrument

Model Bruker AVANCE (800 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Concentration
1	Copper-transporting ATPase 1 (E.C.3.6.3.4)	1 mM
2	phosphate_buffer	100 mM
3	H2O	90 %
4	D2O	10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
5	Copper-transporting ATPase 1 (E.C.3.6.3.4)	[U-15N]	1 mM
6	phosphate_buffer		100 mM
7	DTT		1 mM
8	H2O		90 %
9	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
10	Copper-transporting ATPase 1 (E.C.3.6.3.4)	[U-15N; U-13C]	1 mM
11	phosphate_buffer		100 mM
12	DTT		1 mM
13	H2O		90 %
14	D2O		10 %

nullKeywords Menkes disease, NMR, solution structure, SPINE, Structural Genomics, Structural Proteomics in Europe

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (1.656 seconds)

BMRB: 7069

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Copper-transporting ATPase 1 (E.C.3.6.3.4), : 1 : 1 : 2 : 64 entities Detail

Interaction partners 1. Copper-transporting ATPase 1 (E.C.3.6.3.4), : 5 interactors Detail

Experiments performed 7 experiments Detail

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3