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BMRB: 7081

2JQD

1H, 13C, and 15N Chemical Shift Assignment of the N-terminal domain of LANP

Authors

de Chiara, C., Kelly, G., Frenkiel, T.A., Pastore, A.

Assembly

LANP N-terminal domain (monomeric polymer)

Entity

1. LANP N-terminal domain (monomeric polymer) (polymer, Thiol state: all free), 164 monomers, 18778.27 Da Detail

MEMDKRIYLE LRNRTPSDVK ELVLDNCKSI EGKIEGLTDE FEELEFLSTI NVGLTSISNL PKLNKLKKLE LSENRISGDL EVLAEKCPNL KHLNLSGNKI KDLSTIEPLK KLENLKSLDL FNCEVTNLNA YRENVFKLLP QVMYLDGYDR DNKEAPDSDV EGYV

Formula weight

18778.27 Da

Source organism

Mus musculus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 95.0 %, Completeness (bb): 95.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	95.0 % (1872 of 1970)	94.9 % (983 of 1036)	95.0 % (722 of 760)	96.0 % (167 of 174)
Backbone	95.3 % (926 of 972)	95.7 % (315 of 329)	94.8 % (460 of 485)	95.6 % (151 of 158)
Sidechain	94.0 % (1086 of 1155)	93.5 % (661 of 707)	94.9 % (410 of 432)	93.8 % (15 of 16)
Aromatic	100.0 % (84 of 84)	100.0 % (42 of 42)	100.0 % (42 of 42)
Methyl	97.0 % (192 of 198)	97.0 % (96 of 99)	97.0 % (96 of 99)

1. LANP

MEMDKRIYLE LRNRTPSDVK ELVLDNCKSI EGKIEGLTDE FEELEFLSTI NVGLTSISNL PKLNKLKKLE LSENRISGDL EVLAEKCPNL KHLNLSGNKI KDLSTIEPLK KLENLKSLDL FNCEVTNLNA YRENVFKLLP QVMYLDGYDR DNKEAPDSDV EGYV

Show sample condition

Sample

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	LANP	[U-15N]	0.5 mM
2	TRIS.HCl		10 mM
3	TCEP		2 mM
4	NaN3		0.02 %
5	H2O		90 %
6	D2O		10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	H2O	protons	0.0 ppm	internal	null	0.2514495
¹H	H2O	protons	4.754 ppm	internal	direct	1.0
¹⁵N	H2O	protons	0.0 ppm	internal	null	0.1013291

Referencing offset: -0.09 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	H2O	protons	0.0 ppm	internal	null	0.2514495
¹H	H2O	protons	4.754 ppm	internal	direct	1.0
¹⁵N	H2O	protons	0.0 ppm	internal	null	0.1013291

Referencing offset: -0.09 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	H2O	protons	0.0 ppm	internal	null	0.2514495
¹H	H2O	protons	4.754 ppm	internal	direct	1.0
¹⁵N	H2O	protons	0.0 ppm	internal	null	0.1013291

Referencing offset: -0.05 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	H2O	protons	0.0 ppm	internal	null	0.2514495
¹H	H2O	protons	4.754 ppm	internal	direct	1.0
¹⁵N	H2O	protons	0.0 ppm	internal	null	0.1013291

Referencing offset: 0.46 ppm, Outliers: 1 Detail

Release date

2007-04-10

Citation 1

NMR assignment of the leucine-rich repeat domain of LANP/Anp32a
de Chiara, C., Kelly, G., Frenkiel, T.A., Pastore, A.
J. Biomol. NMR (2007), 38, 177-177, PubMed 17180445 , DOI 10.1007/s10858-006-9101-2 , Abstract

Show more 2 citaions

Citation 2

Structure of pp32, an acidic nuclear protein which inhibits oncogene-induced formation of transformed foci
Chen, T., Brody, J.R., Romantsev, F.E., Yu, J., Kayler, A.E., Voneiff, E., Kuhajda, F.P., Pasternack, G.R.
Mol. Biol. Cell (1996), 7, 2045-2056, PubMed 8970164 , DOI: , Abstract

Citation 3

The cerebellar leucine-rich acidic nuclear protein interacts with ataxin-1
Matilla, A., Koshy, B.T., Cummings, C.J., Isobe, T., Orr, H.T., Zoghbi, H.Y.
Nature (1997), 389, 974-978, PubMed 9353121 , DOI 10.1038/40159 , Abstract

Hide non-primary 2 citaions

Entries sharing articles Swiss-Prot: 4 entries Detail

  Swiss-Prot: O35381 released on 2004-04-13
    Title AN32A_MOUSE Entity Acidic leucine-rich nuclear phosphoprotein 32 family member A
  Swiss-Prot: P54253 released on 1996-10-01
    Title ATX1_HUMAN Entity Ataxin-1
  Swiss-Prot: P54254 released on 1996-10-01
    Title ATX1_MOUSE Entity Ataxin-1
  Swiss-Prot: P39687 released on 1995-02-01
    Title AN32A_HUMAN Entity Acidic leucine-rich nuclear phosphoprotein 32 family member A

Related entities 1. LANP N-terminal domain (monomeric polymer), : 1 : 2 : 2 : 75 entities Detail

Interaction partners 1. LANP N-terminal domain (monomeric polymer), : 13 interactors Detail

More details for 13 interactors identified by 7 citations

Experiments performed 15 experiments Detail

1 1H15N HSQC

Instrument

#1 Model Varian INOVA (800 MHz) Details cryo-probe equipped

#2 Model Varian INOVA (600 MHz)

#3 Model Bruker AVANCE (600 MHz) Details cryo-probe equipped

Sample #1

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	LANP	[U-15N]	0.5 mM
2	TRIS.HCl		10 mM
3	TCEP		2 mM
4	NaN3		0.02 %
5	H2O		90 %
6	D2O		10 %

Sample #2

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
7	LANP	[U-13C; U-15N]	0.5 mM
8	TRIS.HCl		10 mM
9	TCEP		2 mM
10	NaN3		0.02 %
11	H2O		90 %
12	D2O		10 %

2 1H15N NOESY HSQC

Instrument

#1 Model Varian INOVA (800 MHz) Details cryo-probe equipped

#2 Model Varian INOVA (600 MHz)

#3 Model Bruker AVANCE (600 MHz) Details cryo-probe equipped

Sample #1

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	LANP	[U-15N]	0.5 mM
2	TRIS.HCl		10 mM
3	TCEP		2 mM
4	NaN3		0.02 %
5	H2O		90 %
6	D2O		10 %

Sample #2

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
7	LANP	[U-13C; U-15N]	0.5 mM
8	TRIS.HCl		10 mM
9	TCEP		2 mM
10	NaN3		0.02 %
11	H2O		90 %
12	D2O		10 %

3 1H15N TOCSY HSQC

Instrument

#1 Model Varian INOVA (800 MHz) Details cryo-probe equipped

#2 Model Varian INOVA (600 MHz)

#3 Model Bruker AVANCE (600 MHz) Details cryo-probe equipped

Sample #1

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	LANP	[U-15N]	0.5 mM
2	TRIS.HCl		10 mM
3	TCEP		2 mM
4	NaN3		0.02 %
5	H2O		90 %
6	D2O		10 %

Sample #2

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
7	LANP	[U-13C; U-15N]	0.5 mM
8	TRIS.HCl		10 mM
9	TCEP		2 mM
10	NaN3		0.02 %
11	H2O		90 %
12	D2O		10 %

4 1H13C HSQC

Instrument

#1 Model Varian INOVA (800 MHz) Details cryo-probe equipped

#2 Model Varian INOVA (600 MHz)

#3 Model Bruker AVANCE (600 MHz) Details cryo-probe equipped

Sample #1

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	LANP	[U-15N]	0.5 mM
2	TRIS.HCl		10 mM
3	TCEP		2 mM
4	NaN3		0.02 %
5	H2O		90 %
6	D2O		10 %

Sample #2

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
7	LANP	[U-13C; U-15N]	0.5 mM
8	TRIS.HCl		10 mM
9	TCEP		2 mM
10	NaN3		0.02 %
11	H2O		90 %
12	D2O		10 %

5 HCCH TOCSY

Instrument

#1 Model Varian INOVA (800 MHz) Details cryo-probe equipped

#2 Model Varian INOVA (600 MHz)

#3 Model Bruker AVANCE (600 MHz) Details cryo-probe equipped

Sample #1

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	LANP	[U-15N]	0.5 mM
2	TRIS.HCl		10 mM
3	TCEP		2 mM
4	NaN3		0.02 %
5	H2O		90 %
6	D2O		10 %

Sample #2

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
7	LANP	[U-13C; U-15N]	0.5 mM
8	TRIS.HCl		10 mM
9	TCEP		2 mM
10	NaN3		0.02 %
11	H2O		90 %
12	D2O		10 %

6 1H13C NOESY HSQC

Instrument

#1 Model Varian INOVA (800 MHz) Details cryo-probe equipped

#2 Model Varian INOVA (600 MHz)

#3 Model Bruker AVANCE (600 MHz) Details cryo-probe equipped

Sample #1

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	LANP	[U-15N]	0.5 mM
2	TRIS.HCl		10 mM
3	TCEP		2 mM
4	NaN3		0.02 %
5	H2O		90 %
6	D2O		10 %

Sample #2

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
7	LANP	[U-13C; U-15N]	0.5 mM
8	TRIS.HCl		10 mM
9	TCEP		2 mM
10	NaN3		0.02 %
11	H2O		90 %
12	D2O		10 %

7 HNCO

Instrument

#1 Model Varian INOVA (800 MHz) Details cryo-probe equipped

#2 Model Varian INOVA (600 MHz)

#3 Model Bruker AVANCE (600 MHz) Details cryo-probe equipped

Sample #1

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	LANP	[U-15N]	0.5 mM
2	TRIS.HCl		10 mM
3	TCEP		2 mM
4	NaN3		0.02 %
5	H2O		90 %
6	D2O		10 %

Sample #2

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
7	LANP	[U-13C; U-15N]	0.5 mM
8	TRIS.HCl		10 mM
9	TCEP		2 mM
10	NaN3		0.02 %
11	H2O		90 %
12	D2O		10 %

8 HNCA

Instrument

#1 Model Varian INOVA (800 MHz) Details cryo-probe equipped

#2 Model Varian INOVA (600 MHz)

#3 Model Bruker AVANCE (600 MHz) Details cryo-probe equipped

Sample #1

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	LANP	[U-15N]	0.5 mM
2	TRIS.HCl		10 mM
3	TCEP		2 mM
4	NaN3		0.02 %
5	H2O		90 %
6	D2O		10 %

Sample #2

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
7	LANP	[U-13C; U-15N]	0.5 mM
8	TRIS.HCl		10 mM
9	TCEP		2 mM
10	NaN3		0.02 %
11	H2O		90 %
12	D2O		10 %

9 HN(CO)CA

Instrument

#1 Model Varian INOVA (800 MHz) Details cryo-probe equipped

#2 Model Varian INOVA (600 MHz)

#3 Model Bruker AVANCE (600 MHz) Details cryo-probe equipped

Sample #1

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	LANP	[U-15N]	0.5 mM
2	TRIS.HCl		10 mM
3	TCEP		2 mM
4	NaN3		0.02 %
5	H2O		90 %
6	D2O		10 %

Sample #2

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
7	LANP	[U-13C; U-15N]	0.5 mM
8	TRIS.HCl		10 mM
9	TCEP		2 mM
10	NaN3		0.02 %
11	H2O		90 %
12	D2O		10 %

10 CBCANH

Instrument

#1 Model Varian INOVA (800 MHz) Details cryo-probe equipped

#2 Model Varian INOVA (600 MHz)

#3 Model Bruker AVANCE (600 MHz) Details cryo-probe equipped

Sample #1

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	LANP	[U-15N]	0.5 mM
2	TRIS.HCl		10 mM
3	TCEP		2 mM
4	NaN3		0.02 %
5	H2O		90 %
6	D2O		10 %

Sample #2

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
7	LANP	[U-13C; U-15N]	0.5 mM
8	TRIS.HCl		10 mM
9	TCEP		2 mM
10	NaN3		0.02 %
11	H2O		90 %
12	D2O		10 %

11 CBCA(CO)NH

Instrument

#1 Model Varian INOVA (800 MHz) Details cryo-probe equipped

#2 Model Varian INOVA (600 MHz)

#3 Model Bruker AVANCE (600 MHz) Details cryo-probe equipped

Sample #1

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	LANP	[U-15N]	0.5 mM
2	TRIS.HCl		10 mM
3	TCEP		2 mM
4	NaN3		0.02 %
5	H2O		90 %
6	D2O		10 %

Sample #2

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
7	LANP	[U-13C; U-15N]	0.5 mM
8	TRIS.HCl		10 mM
9	TCEP		2 mM
10	NaN3		0.02 %
11	H2O		90 %
12	D2O		10 %

12 (HB)CB(CGCD)HD

Instrument

#1 Model Varian INOVA (800 MHz) Details cryo-probe equipped

#2 Model Varian INOVA (600 MHz)

#3 Model Bruker AVANCE (600 MHz) Details cryo-probe equipped

Sample #1

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	LANP	[U-15N]	0.5 mM
2	TRIS.HCl		10 mM
3	TCEP		2 mM
4	NaN3		0.02 %
5	H2O		90 %
6	D2O		10 %

Sample #2

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
7	LANP	[U-13C; U-15N]	0.5 mM
8	TRIS.HCl		10 mM
9	TCEP		2 mM
10	NaN3		0.02 %
11	H2O		90 %
12	D2O		10 %

13 (HB)CB(CGCD)HE

Instrument

#1 Model Varian INOVA (800 MHz) Details cryo-probe equipped

#2 Model Varian INOVA (600 MHz)

#3 Model Bruker AVANCE (600 MHz) Details cryo-probe equipped

Sample #1

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	LANP	[U-15N]	0.5 mM
2	TRIS.HCl		10 mM
3	TCEP		2 mM
4	NaN3		0.02 %
5	H2O		90 %
6	D2O		10 %

Sample #2

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
7	LANP	[U-13C; U-15N]	0.5 mM
8	TRIS.HCl		10 mM
9	TCEP		2 mM
10	NaN3		0.02 %
11	H2O		90 %
12	D2O		10 %

14 C(CO)NH

Instrument

#1 Model Varian INOVA (800 MHz) Details cryo-probe equipped

#2 Model Varian INOVA (600 MHz)

#3 Model Bruker AVANCE (600 MHz) Details cryo-probe equipped

Sample #1

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	LANP	[U-15N]	0.5 mM
2	TRIS.HCl		10 mM
3	TCEP		2 mM
4	NaN3		0.02 %
5	H2O		90 %
6	D2O		10 %

Sample #2

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
7	LANP	[U-13C; U-15N]	0.5 mM
8	TRIS.HCl		10 mM
9	TCEP		2 mM
10	NaN3		0.02 %
11	H2O		90 %
12	D2O		10 %

15 H(CCO)NH

Instrument

#1 Model Varian INOVA (800 MHz) Details cryo-probe equipped

#2 Model Varian INOVA (600 MHz)

#3 Model Bruker AVANCE (600 MHz) Details cryo-probe equipped

Sample #1

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	LANP	[U-15N]	0.5 mM
2	TRIS.HCl		10 mM
3	TCEP		2 mM
4	NaN3		0.02 %
5	H2O		90 %
6	D2O		10 %

Sample #2

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
7	LANP	[U-13C; U-15N]	0.5 mM
8	TRIS.HCl		10 mM
9	TCEP		2 mM
10	NaN3		0.02 %
11	H2O		90 %
12	D2O		10 %

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr7081_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr7081_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	chem_shift_list_1	Warning	2075		100.0 (chain: 1, length: 164)
1	Chemical shift references	chemical_shift_referencing	OK	3		No information

Assigned chemical shifts

Saveframe: chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 164, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 1194
  - ¹³C chemical shifts: 713
  - ¹⁵N chemical shifts: 168
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	1036	990	95.6
¹³C chemical shifts	760	713	93.8
¹⁵N chemical shifts	180	168	93.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	329	320	97.3
¹³C chemical shifts	328	302	92.1
¹⁵N chemical shifts	158	150	94.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	707	670	94.8
¹³C chemical shifts	432	411	95.1
¹⁵N chemical shifts	22	18	81.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	102	102	100.0
¹³C chemical shifts	102	101	99.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	42	42	100.0
¹³C chemical shifts	42	42	100.0

Keywords assignment, NMR

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Found 1 Document (2.876 seconds)

BMRB: 7081

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles Swiss-Prot: 4 entries Detail

Related entities 1. LANP N-terminal domain (monomeric polymer), : 1 : 2 : 2 : 75 entities Detail

Interaction partners 1. LANP N-terminal domain (monomeric polymer), : 13 interactors Detail

Experiments performed 15 experiments Detail

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Chemical shift validation 3 contents Detail