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BMRB: 7105

2GZK

1H, 13C and 15N chemical shift assignments for SRY.B in complex with 16-mer DNA

Authors

Stott, K., Tang, G.S., Lee, K., Thomas, J.O.

Assembly

SRY.B in complex with 16-mer DNA

Entity

1. SRY.B (polymer, Thiol state: all free), 159 monomers, 18947.68 Da Detail

VQDRVKRPMN AFIVWSRDQR RKMALENPRM RNSEISKQLG YQWKMLTEAE KWPFFQEAQK LQAMHREKYP NYKYRKGETK KKFKDPNAPK RPPSAFFLFC SEYRPKIKGE HPGLSIGDVA KKLGEMWNNT AADDKQPYEK KAAKLKEKYE KDIAAYRAK

2. 16-mer DNA trand 1 (polymer, Thiol state: not present), 16 monomers, 4994.202 Da Detail

GGGATCTAAA CAATGC

3. 16-mer DNA strand 2 (polymer, Thiol state: not present), 16 monomers, 4927.136 Da Detail

GCATTGTTTA GATCCC

Total weight

28869.02 Da

Max. entity weight

18947.68 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 91.7 %, Completeness (bb): 87.9 % Detail

Polymer type: polypeptide(L) polydeoxyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	91.7 % (2145 of 2339)	95.2 % (1336 of 1403)	83.9 % (645 of 769)	98.2 % (164 of 167)
Backbone	87.9 % (1016 of 1156)	96.5 % (518 of 537)	74.5 % (351 of 471)	99.3 % (147 of 148)
Sidechain	95.9 % (1281 of 1336)	94.5 % (818 of 866)	98.9 % (446 of 451)	89.5 % (17 of 19)
Aromatic	96.9 % (246 of 254)	96.2 % (153 of 159)	97.8 % (89 of 91)	100.0 % (4 of 4)
Methyl	100.0 % (113 of 113)	100.0 % (61 of 61)	100.0 % (52 of 52)

1. SRY.B

VQDRVKRPMN AFIVWSRDQR RKMALENPRM RNSEISKQLG YQWKMLTEAE KWPFFQEAQK LQAMHREKYP NYKYRKGETK KKFKDPNAPK RPPSAFFLFC SEYRPKIKGE HPGLSIGDVA KKLGEMWNNT AADDKQPYEK KAAKLKEKYE KDIAAYRAK

2. 16-mer DNA trand 1

GGGATCTAAA CAATGC

3. 16-mer DNA strand 2

GCATTGTTTA GATCCC

Show sample condition

Sample

Temperature 310 K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	SRY.B	[U-99% 13C; U-99% 15N]	1 mM
2	Sodium phosphate		10 mM
3	EDTA		1 mM
4	DTT	[U-98% 2H]	2 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.4 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.4 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.1 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 1 models in PDB: 2GZK, Strand ID: A Detail

Release date

2006-08-06

Citation

Structure of a complex of tandem HMG boxes and DNA
Stott, K., Tang, G.S.F., Lee, K., Thomas, J.O.
J. Mol. Biol. (2006), 360, 90-104, PubMed 16813837 , DOI 10.1016/j.jmb.2006.04.059 , Abstract

Related entities 1. SRY.B, : 1 : 77 entities Detail

Experiments performed 20 experiments Detail

1 2D HSQC