BMRBj - BMREntryMaster

Found 1 Document (1.514 seconds)

BMRB: 7159

9B7F

1H chemical shift assignments of 2SS[6-127, 30-115] at pH 3.8 and 25 C

Authors

Noda, Y., Yokota, A., Horii, D., Tominaga, T., Tanisaka, Y., Tachibana, H., Segawa, S.

Assembly

2SS[6-127, 30-115]

Entity

1. 2SS[6-127, 30-115] (polymer, Thiol state: unknown), 129 monomers, 14184.75 Da Detail

KVFGRCELAA AMKRHGLDNY RGYSLGNWVC AAKFESNFNT QATNRNTDGS TDYGILQINS RWWANDGRTP GSRNLANIPA SALLSSDITA SVNAAKKIVS DGNGMNAWVA WRNRCKGTDV QAWIRGCRL

Formula weight

14184.75 Da

Source organism

Gallus gallus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 85.3 %, Completeness: 66.2 %, Completeness (bb): 82.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H
All	66.2 % (495 of 748)	66.2 % (495 of 748)
Backbone	82.8 % (222 of 268)	82.8 % (222 of 268)
Sidechain	56.9 % (273 of 480)	56.9 % (273 of 480)
Aromatic	76.9 % (50 of 65)	76.9 % (50 of 65)
Methyl	79.4 % (50 of 63)	79.4 % (50 of 63)

1. 2SS[6-127, 30-115]

KVFGRCELAA AMKRHGLDNY RGYSLGNWVC AAKFESNFNT QATNRNTDGS TDYGILQINS RWWANDGRTP GSRNLANIPA SALLSSDITA SVNAAKKIVS DGNGMNAWVA WRNRCKGTDV QAWIRGCRL

Show sample condition

Sample

Temperature 298 (±0) K, pH 3.8 (±0)

#	Name	Concentration
1	2SS[6-127, 30-115]	1.2 mM
2	H2O	95 %
3	D2O	5 %

Release date

2008-11-11

Citation

NMR structural study of two-disulfide variant of hen lysozyme: 2SS[6-127, 30-115]--a disulfide intermediate with a partly unfolded structure
Noda, Y., Yokota, A., Horii, D., Tominaga, T., Tanisaka, Y., Tachibana, H., Segawa, S.
Biochemistry (2002), 41, 2130-2139, PubMed 11841203 , Abstract

Related entities 1. 2SS[6-127, 30-115], : 1 : 105 : 141 entities Detail

Interaction partners 1. 2SS[6-127, 30-115], : 7 interactors Detail

More details for 7 interactors identified by 6 citations

Experiments performed 1 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr7159_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr7159_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	chem_shift_list_1	Warning	592		85.3 (chain: 1, length: 129)
1	Chemical shift references	chemical_shift_referencing	OK	1		No information

Assigned chemical shifts

Saveframe: chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 129, Sequence coverage: 85.3%
- Total number of assignments
  - ¹H chemical shifts: 592
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

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Found 1 Document (1.514 seconds)

BMRB: 7159

Sample

Related entities 1. 2SS[6-127, 30-115], : 1 : 105 : 141 entities Detail

Interaction partners 1. 2SS[6-127, 30-115], : 7 interactors Detail

Experiments performed 1 experiments Detail

Instrument

Sample

Chemical shift validation 3 contents Detail