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BMRB: 7206

2HKY

Backbone 1H, 13C, and 15N Chemical Shift Assignments for Human RNase 7

Authors

Huang, Y.

Assembly

human RNase 7

Entity

1. human RNase 7 (polymer, Thiol state: all disulfide bound), 129 monomers, 14684.95 Da Detail

MKPKGMTSSQ WFKIQHMQPS PQACNSAMKN INKHTKRCKD LNTFLHEPFS SVAATCQTPK IACKNGDKNC HQSHGPVSLT MCKLTSGKYP NCRYKEKRQN KSYVVACKPP QKKDSQQFHL VPVHLDRVL

Formula weight

14684.95 Da

Entity Connection

disulfide 4 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS56:SG	1:CYS107:SG
2	disulfide	sing	1:CYS38:SG	1:CYS92:SG
3	disulfide	sing	1:CYS63:SG	1:CYS70:SG
4	disulfide	sing	1:CYS24:SG	1:CYS82:SG

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

distance geometry, simulated annealing, torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.2 %, Completeness: 90.8 %, Completeness (bb): 98.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	90.8 % (1412 of 1555)	92.6 % (767 of 828)	89.5 % (527 of 589)	85.5 % (118 of 138)
Backbone	98.0 % (739 of 754)	97.2 % (245 of 252)	98.4 % (377 of 383)	98.3 % (117 of 119)
Sidechain	85.1 % (788 of 926)	89.9 % (518 of 576)	81.6 % (270 of 331)	0.0 % (0 of 19)
Aromatic	43.3 % (45 of 104)	86.5 % (45 of 52)	0.0 % (0 of 51)	0.0 % (0 of 1)
Methyl	97.9 % (92 of 94)	95.7 % (45 of 47)	100.0 % (47 of 47)

1. human RNase 7

MKPKGMTSSQ WFKIQHMQPS PQACNSAMKN INKHTKRCKD LNTFLHEPFS SVAATCQTPK IACKNGDKNC HQSHGPVSLT MCKLTSGKYP NCRYKEKRQN KSYVVACKPP QKKDSQQFHL VPVHLDRVL

Show sample condition

Sample

Temperature 310 (±0.1) K, pH 3.5 (±0.01)

#	Name	Isotope labeling	Type	Concentration
1	human RNase 7	[U-95% 13C; U-90% 15N]		0.6 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.2 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.2 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.17 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 15 models in PDB: 2HKY, Strand ID: A Detail

Release date

2006-08-13

Citation

The flexible and clustered lysine residues of human ribonuclease 7 are critical for membrane permeability and antimicrobial activity
Huang, Y., Lin, Y., Chang, T., Wu, S., Lee, Y., Yu, H., Chang, D., Chen, C., Wu, S., Liao, Y.
J. Biol. Chem. (2007), 282, 4626-4633, PubMed 17150966 , DOI 10.1074/jbc.M607321200 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: Q9H1E1 released on 2001-12-19
Title RNAS7_HUMAN Entity Ribonuclease 7

Related entities 1. human RNase 7, : 1 : 236 entities Detail

Experiments performed 13 experiments Detail

1 2D TOCSY