BMRB: 7262

NMR assignments of the the N-terminal subdomain of IGFBP-6

Authors

Chandrashekaran, I.R., Yao, S., Wang, C.C., Bansal, P.S., Alewood, P.F., Wallace, J.C., Bach, L.A., Norton, R.S.

Assembly

1-45IGFBP-6

Entity

1. 1-45IGFBP-6 (polymer, Thiol state: all disulfide bound), 45 monomers, 4365.733 Da Detail

ALARCPGCGQ GVQAGCPGGC VEEEDGGSPA EGCAEAEGCL RREGQ

Formula weight

4365.733 Da

Entity Connection

disulfide 3 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS5:SG	1:CYS8:SG
2	disulfide	sing	1:CYS16:SG	1:CYS20:SG
3	disulfide	sing	1:CYS33:SG	1:CYS39:SG

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 59.0 %, Completeness (bb): 61.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	59.0 % (249 of 422)	80.1 % (177 of 221)	23.7 % (37 of 156)	77.8 % (35 of 45)
Backbone	61.7 % (163 of 264)	92.9 % (91 of 98)	29.8 % (37 of 124)	83.3 % (35 of 42)
Sidechain	52.1 % (100 of 192)	69.9 % (86 of 123)	21.2 % (14 of 66)	0.0 % (0 of 3)
Methyl	53.6 % (15 of 28)	78.6 % (11 of 14)	28.6 % (4 of 14)

1. NN-BP-6

ALARCPGCGQ GVQAGCPGGC VEEEDGGSPA EGCAEAEGCL RREGQ

Show sample condition

Sample

Temperature 288 (±0.2) K, pH 4.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	NN-BP-6		protein	1.1 mM
2	Sodium acetate		buffer	10 mM
3	Sodium azide			0.02 %
4	D2O			5 %

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Calculated from 20 models in PDB: 2JM2, Strand ID: A Detail

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Coupling constant

15 J values in 1 lists
Field strength (¹H) 600 MHz, Temperature 288 (±0.2) K, pH 4.3 (±0.1) Detail

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Temperature 288 (±0.2) K, pH 4.3 (±0.1)

Experiment name 2D 1H-1H DQF-COSY

List #1 J_coupling_list_1, Field strength (¹H) 600 MHz

Release date

2007-03-05

Citation

The N-terminal subdomain of insulin-like growth factor (IGF) binding protein 6. Structure and interaction with IGFs
Chandrashekaran, I.R., Yao, S., Wang, C.C., Bansal, P.S., Alewood, P.F., Forbes, B.E., Wallace, J.C., Bach, L.A., Norton, R.S.
Biochemistry (2007), 46, 3065-3074, PubMed 17305365 , DOI 10.1021/bi0619876 , Abstract

Related entities 1. 1-45IGFBP-6, : 1 : 2 : 1 : 8 entities Detail

Interaction partners 1. 1-45IGFBP-6, : 1 interactors Detail

Experiments performed 5 experiments Detail